There are several low lying singlet oxygen (1O2) and superoxide radical (O-2) which are important in photochemical oxidation. In our present analysis we are studying chemical property of singlet oxygen (1O2) and super oxide radical (O-2) and some dyestuffs species to produce reactive oxygen such as singlet oxygen1O2and superoxide radicals. Irradiation with sun lightin vitrothe dyestuff like benzanthrone, metanil yellow andp-aminodiphenylamine were found to produce reactive oxygen species such as singlet oxygen (1O2) and/or superoxide radicals (O-2) .The dose response relationship between singlet1O2production when sunlight expose of those dyestuffs (0-25 min), and (0-12 min) for super oxide (O-2) production were studied. However benzanthrone produces detectable amount of1O2, Although metanil yellow andp-aminodiphenylamine (p-ADPA) did not produce detectable amounts of1O2under similar conditions. The above dye stuffs are routinely used in textiles, cosmetics, detergents, leather industries as well as food additives and role of these activated oxygen species in the development of skin diseases.