A high resolution SEM study of the effects of RU486, used as a postcoital contraceptive, on the rat uterus during early pregnancy

2008 ◽  
Vol 32 (4) ◽  
pp. 436-446 ◽  
Author(s):  
Kathrine E. Scholtz ◽  
Clem B. Penny ◽  
Margot J. Hosie
Author(s):  
M. Müller ◽  
R. Hermann

Three major factors must be concomitantly assessed in order to extract relevant structural information from the surface of biological material at high resolution (2-3nm).Procedures based on chemical fixation and dehydration in graded solvent series seem inappropriate when aiming for TEM-like resolution. Cells inevitably shrink up to 30-70% of their initial volume during gehydration; important surface components e.g. glycoproteins may be lost. These problems may be circumvented by preparation techniques based on cryofixation. Freezedrying and freeze-substitution followed by critical point drying yields improved structural preservation in TEM. An appropriate preservation of dimensional integrity may be achieved by freeze-drying at - 85° C. The sample shrinks and may partially collapse as it is warmed to room temperature for subsequent SEM study. Observations at low temperatures are therefore a necessary prerequisite for high fidelity SEM. Compromises however have been unavoidable up until now. Aldehyde prefixation is frequently needed prior to freeze drying, rendering the sample resistant to treatment with distilled water.


Author(s):  
D.F. Bowling

High school cosmetology students study the methods and effects of various human hair treatments, including permanents, straightening, conditioning, coloring and cutting. Although they are provided with textbook examples of overtreatment and numerous hair disorders and diseases, a view of an individual hair at the high resolution offered by an SEM provides convincing evidence of the hair‘s altered structure. Magnifications up to 2000X provide dramatic differences in perspective. A good quality classroom optical microscope can be very informative at lower resolutions.Students in a cosmetology class are initially split into two groups. One group is taught basic controls on the SEM (focus, magnification, brightness, contrast, specimen X, Y, and Z axis movements). A healthy, untreated piece of hair is initially examined on the SEM The second group cements a piece of their own hair on a stub. The samples are dryed quickly using heat or vacuum while the groups trade places and activities.


1998 ◽  
Vol 7 (2) ◽  
pp. 109-114 ◽  
Author(s):  
Gunther Mielke ◽  
Ludwig Kiesel ◽  
Claudia Backsch ◽  
Winfried Erz ◽  
Markus Gonser

2008 ◽  
Vol 54 (5) ◽  
pp. 340-345 ◽  
Author(s):  
Kaiyu KUBOTA ◽  
Nobuhiko YAMAUCHI ◽  
Kenji MATSUMOTO ◽  
Ryo WATANABE ◽  
Shinji OOZONO ◽  
...  
Keyword(s):  

2011 ◽  
Vol 345 (2) ◽  
pp. 275-284 ◽  
Author(s):  
Kyohei Nishimura ◽  
Nobuhiko Yamauchi ◽  
Vishwajit Sur Chowdhury ◽  
Mikinori Torii ◽  
Masa-aki Hattori ◽  
...  

2001 ◽  
Vol 29 (7) ◽  
pp. 499-507 ◽  
Author(s):  
Lorenzo Breschi ◽  
Pietro Gobbi ◽  
Mirella Falconi ◽  
Luigi Ciavarelli ◽  
Gabriella Teti ◽  
...  
Keyword(s):  

2003 ◽  
Vol 9 (S03) ◽  
pp. 502-503 ◽  
Author(s):  
Jaap Nijsse ◽  
Paul Walther ◽  
Elena Golovina ◽  
Folkert A. Hoekstra

1969 ◽  
Vol 61 (1_Suppl) ◽  
pp. S248
Author(s):  
Krystyna Malinowska ◽  
Rosalind Greenstreet ◽  
K. Fotherby

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