The mechanical responses of Ti-5Al-2.5Sn alloy at low temperatures were investigated under quasi-static and dynamic tensile loads using MTS system and SHTB system, respectively. Tensile stress-strain curves were obtained over the temperature range of 153 to 298K and the rate range of 0.001 to 1050 s-1. Experimental results indicate that the tensile behavior of Ti-5Al-2.5Sn alloy is dependent on strain rate and temperature. Yield stress and flow stress increase with increasing strain rate and decrease with increasing temperature. Results also indicate that strain hardening rate of Ti-5Al-2.5Sn alloy is lower at high strain rate, while strain hardening rate varies little with testing temperature. The Khan-Huang-Liang constitutive model was chosen to characterize the tensile responses of Ti-5Al-2.5Sn alloy at low temperatures and different strain rates. The model results coincide well with the experimental results within the tested temperature and rate ranges.