Effect mechanism of multiple obstacles on non-Newtonian flow in ceramics 3D printing (linear elements)

The multi-relaxation time lattice Boltzmann method (MRT-LBM) has an excellent performance in dealing with the complex flow in many different areas. According to the specific behavior of the fluids, it also has some shortcomings when applied to some special flow like as the non-Newtonian flow. In Cement-3D printing, the fluids always exhibit according to the yielding behavior. When using the standard MRT-LBM, the simulation maybe divergent. In order to solve the problem, this work presents an improved MRT-LBM considering the non-Newtonian effect as a special forcing term to ensure the stable and accurate simulation. Finally, the Poiseuille flow was used to validate the feasibility of the proposed method.

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The application of video-enhanced constrast/differential interference constrast microscopy in conjunction with whole mount electron microscopy to the study of organelle transport

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100102419 ◽

1988 ◽

Vol 46 ◽

pp. 66-67

Author(s):

J. H. Hayden

Keyword(s):

Electron Microscopy ◽

Rana Pipiens ◽

Silver Film ◽

Immunofluorescence Microscopy ◽

Organelle Transport ◽

Linear Element ◽

Whole Mount ◽

Carbon Coated ◽

Linear Elements ◽

Dic Microscopy

In a previous study, Allen video-enhanced constrast/differential interference constrast (AVEC-DIC) microscopy was used in conjunction with immunofluorescence microscopy to demonstrate that organelles and vesicle move in either direction along linear elements composed of microtubules. However, this study was limited in that the number of microtubules making up a linear element could not be determined. To overcome this limitation, we have used AVEC-DIC microscopy in conjunction with whole mount electron microscopy.Keratocytes from Rana pipiens were grown on glass coverslips as described elsewhere. Gold London Finder grids were Formvar- and carbon coated, and sterilized by exposure to ultraviolet light. It is important to select a Formvar film that gives a grey reflection when it is floated on water. A silver film is too thick and will detract from the image in the light microscope.

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