Determination of n-octanol/water partition coefficient for DDT-related compounds by RP-HPLC with a novel dual-point retention time correction

Chemosphere ◽  
2011 ◽  
Vol 83 (2) ◽  
pp. 131-136 ◽  
Author(s):  
Shu-ying Han ◽  
Jun-qin Qiao ◽  
Yun-yang Zhang ◽  
Li-li Yang ◽  
Hong-zhen Lian ◽  
...  
Keyword(s):  
Partition Coefficient ◽  
Retention Time ◽  
Rp Hplc ◽  
Water Partition Coefficient ◽  
Time Correction ◽  
Related Compounds

scholarly journals RP-HPLC Determination of Raloxifene in Pharmaceuticl Tablets

2006 ◽  
Vol 3 (1) ◽  
pp. 60-64 ◽  
Author(s):  
P. Venkata Reddy ◽  
B. Sudha Rani ◽  
G. Srinu Babu ◽  
J. V. L. N. Seshagiri Rao
Keyword(s):  
Flow Rate ◽  
Retention Time ◽  
Mobile Phase ◽  
Dosage Forms ◽  
Hplc Method ◽  
Reverse Phase Hplc ◽  
Reverse Phase ◽  
Pharmaceutical Dosage Forms ◽  
Rp Hplc

A reverse phase HPLC method is developed for the determination of Raloxifene in pharmaceutical dosage forms. Chromatography was carried out on an inertsil C18 column using a mixture of acetonitrile and phosphate buffer (30:70 v/v) as the mobile phase at a flow rate of 1 mL/min. Detection was carried out at 290 nm .The retention time of the drug was 10.609 min. The method produced linear responses in the concentration range of 0.5-200 µg/mL of Raloxifene. The method was found to be applicable for determination of the drug in tablets.

Determination of Ionization Constant (pKa) and Octanol–Water Partition Coefficient (Log P) of Cyclopiazonic Acid

2005 ◽  
Vol 88 (5) ◽  
pp. 1367-1370 ◽  
Author(s):  
Victor S Sobolev
Keyword(s):  
Partition Coefficient ◽  
Potentiometric Titration ◽  
Ionization Constant ◽  
Cyclopiazonic Acid ◽  
Log P ◽  
Experimental Conditions ◽  
Water Partition Coefficient

Abstract The ionization constant (pKa) and the octanol–water partition coefficient (log P) of the important mycotoxin cyclopiazonic acid (CPA) were determined by means of potentiometric titration, and the lipophilicity profile (log D) was calculated. Under the experimental conditions, pKa of CPA = 2.97 ± 0.09, log P = 3.83 ± 0.10, and log D at pH 7.4 = −0.58.

scholarly journals APPLICATION OF VALIDATED RP-HPLC METHOD FOR THE DETERMINATION OF ARMODAFINIL IN BULK AND FORMULATION

2017 ◽  
pp. 158-161
Author(s):  
Devi Ramesh ◽  
Mohammad Habibuddin
Keyword(s):  
Retention Time ◽  
Mobile Phase ◽  
Hplc Method ◽  
Reverse Phase Hplc ◽  
Reverse Phase ◽  
Rp Hplc ◽  
Ich Guidelines ◽  
Validation Parameters ◽  
Isocratic Mode

Objective: The objective of the present study is to develop and validate a simple, rapid, sensitive reverse phase HPLC method for the determination of Armodafinil present in bulk and its pharmaceutical formulations.Methods: The chromatographic separation was achieved by using Hypersil ODS C-18 (150 x 4.6 mm, 5µ) in an isocratic mode with mobile phase methanol: phosphate buffer 3.0 (60:40 %v/v) was used. The flow rate was 1 ml/min and effluent was monitored at 225 nm. The method was validated for validation parameters i.e. linearity, accuracy, precision and robustness according to ICH guidelines.Results: The retention time of Armodafinil was 4.2 min and the linearity range of the method was 500-20000ng/ml with regression (r2) coefficient 0.9998. The method was validated for precision, accuracy, robustness and which were found to be within the acceptable limits according to the ICH guidelines. Also, the method was successfully applied for the estimation of Armodafinil in the marketed formulation of Nuvigil and the recovery was found to be>98%.Conclusion: The developed method possess good selectivity, specificity, there is no interference found in the blank at a retention time of ARM and good correlation between the peak area and concentration of the drugs under prescribed conditions. Hence, the method can be applied for routine analysis of Armodafinil. 

scholarly journals Development of RP-HPLC method for the estimation of Rasagiline mesylate in bulk and tablet dosage forms

2012 ◽  
Vol 1 (9) ◽  
pp. 285-287 ◽  
Author(s):  
Napa Delhi Raj ◽  
Abburu Rukmangada Rao ◽  
Chusena Narasimharaju Bhimanadhuni
Keyword(s):  
Retention Time ◽  
Correlation Coefficients ◽  
Pharmaceutical Journal ◽  
Ammonia Solution ◽  
Hplc Method ◽  
Detection Range ◽  
Rp Hplc ◽  
Rasagiline Mesylate ◽  
Tablet Dosage

A simple RP-HPLC method for the determination of Rasagiline Mesylate in bulk and tablet dosage form was developed. Numerous HPLC conditions were tested for determination of rasagiline. The best result was achieved by using Purosphere star RP-18, (150×4.6mm), 5µm column and a mobile phase consisting of Potassium Orthophosphate: Acetonitrile (60:40 v/v) adjusted to pH 7.0(±0.05) with Ammonia solution, a flow rate of 1.5 ml/min with ultraviolet detection at 210nm. The correlation coefficients for calibration curves within the detection range of 5-30µg/ml were 0.9993. The within and between-day precision was determined for both retention time and peak area. The retention time of rasagiline is 6.0 minutes.DOI: http://dx.doi.org/10.3329/icpj.v1i9.11620 International Current Pharmaceutical Journal 2012, 1(9): 285-287 

Determination of acid base balance and oil water partition coefficient of an atopy patch test of levofloxacin

2008 ◽  
Vol 62 (2) ◽  
pp. 136-138 ◽  
Author(s):  
Julien Lecordier ◽  
Jean-Jacques Lahet ◽  
Carol Courderot-Masuyer ◽  
Etienne Beaudoin ◽  
Bernard Chaillot
Keyword(s):  
Partition Coefficient ◽  
Patch Test ◽  
Atopy Patch Test ◽  
Acid Base Balance ◽  
Acid Base ◽  
Water Partition Coefficient ◽  
Base Balance ◽  
Oil Water

Development of validated method for the determination of exemestane by using RP-HPLC

2018 ◽  
Vol 9 (1) ◽  
pp. 185
Author(s):  
Siva Sai Kiran B ◽  
Raja S
Keyword(s):  
Detection Limit ◽  
Retention Time ◽  
Mobile Phase ◽  
Reversed Phase ◽  
Chromatographic Peak ◽  
Rp Hplc ◽  
Ich Guidelines ◽  
Hplc Grade Water ◽  
Isocratic Mode

A novel RP-HPLC validated method for determination of Exemestane is developed. The chromatographic separation was done on Phenomenex Luna reversed phase C18 (150 mm x 4.6 mm, 5 μm) column in isocratic mode, using Acetonitrile: HPLC grade water (50:50, v/v) as mobile phase at 254 nm wavelength. Exemestane chromatographic peak is eluted with retention time 4.656 min. The linearity range was 5-30 μg/ml with correlation coefficient 0.999. The method was validated as per ICH Guidelines. The quantification and detection limit for estimation of Exemestane was found to be 0.365 and 1.926 μg/ml respectively. Recovery of Exemestane was found in the range of 98.0-102.0%. Keywords: RP-HPLC; Exemestane; Validation; Estimation

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