Enhancing rhizoremediation of petroleum hydrocarbons through bioaugmentation with a plant growth-promoting bacterial consortium

Chemosphere ◽  
2021 ◽  
pp. 133143
Author(s):  
Michael O. Eze ◽  
Volker Thiel ◽  
Grant C. Hose ◽  
Simon C. George ◽  
Rolf Daniel
Keyword(s):  
Plant Growth ◽  
Petroleum Hydrocarbons ◽  
Bacterial Consortium ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Allium cepa L. Inoculation with a Consortium of Plant Growth-Promoting Bacteria: Effects on Plant Growth and Development and Soil Fertility Status and Microbial Community

Proceedings ◽  
2021 ◽  
Vol 66 (1) ◽  
pp. 20
Author(s):  
Marika Pellegrini ◽  
Daniela M. Spera ◽  
Claudia Ercole ◽  
Maddalena del Gallo
Keyword(s):  
Microbial Community ◽  
Plant Growth ◽  
Soil Fertility ◽  
Allium Cepa ◽  
Bacterial Consortium ◽  
Plant Growth Promoting Bacteria ◽  
Allium Cepa L ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Molecular Profiles

The present work was aimed at investigating the effects of a four strains consortium—Azospirillum brasilense, Gluconacetobacter diazotrophicus, Herbaspirillum seropedicae, and Burkholderia ambifaria—on crops of Allium cepa L. and its soil health. The bacterial consortium was inoculated on seeds of two different onion varieties; inoculated seeds and control ones (treated with autoclaved inoculum) were sown in open-field and followed until harvest. Plant growth development parameters, as well as soil physico-chemical and molecular profiles (DNA extraction and 16S community sequencing on the Mi-Seq Illumina platform), were investigated. The results showed a positive influence of bacterial application on plant growth, with increased plant height (+18%), total chlorophylls (+42%), crop yields (+13%), and bulbs dry matter (+3%) than the control. The differences between control and treated experimental conditions were also underlined in the bulb extracts in terms of total phenolic contents (+25%) and antioxidant activities (+20%). Soil fertility and microbial community structure and diversity were also positively affected by the bacterial inoculum. At harvest, the soil with the presence of the bacterial consortium showed an increase of total organic carbon, organic matter, and available P and higher concentrations of nutrients than control. The ecological indexes calculated on the molecular profiles showed that community diversity was positively affected by the bacterial treatment. The present work allowed to remark the effective use of plant growth-promoting bacteria as a valid fertilization strategy to improve yield in productive landscapes, whilst safeguarding soil biodiversity.

scholarly journals Allium cepa L. Inoculation with a Consortium of Plant Growth-Promoting Bacteria: Effects on Plants, Soil, and the Autochthonous Microbial Community

2021 ◽  
Vol 9 (3) ◽  
pp. 639
Author(s):  
Marika Pellegrini ◽  
Daniela M. Spera ◽  
Claudia Ercole ◽  
Maddalena Del Gallo
Keyword(s):  
Microbial Community ◽  
Plant Growth ◽  
Allium Cepa ◽  
Bacterial Consortium ◽  
Total Phenolic ◽  
Plant Growth Promoting Bacteria ◽  
Allium Cepa L ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Molecular Profiles

The present work was aimed at investigating the effects of a four bacterial strain consortium—Azospirillum brasilense, Gluconacetobacter diazotrophicus, Herbaspirillum seropedicae, and Burkholderia ambifaria—on Allium cepa L. and on soil health. The bacterial consortium was inoculated on seeds of two different onion varieties; inoculated and Control seeds (treated with autoclaved inoculum) were sown in an open-field and followed until harvest. Plant growth development parameters, as well as soil physico–chemical and molecular profiles (DNA extraction and 16S community sequencing on the Mi-Seq Illumina platform), were investigated. The results showed a positive influence of bacterial application on plant growth, with increased plant height (+18%), total chlorophylls (+42%), crop yields (+13%), and bulb dry matter (+3%) with respect to the Control. The differences between Control and treatments were also underlined in the bulb extracts in terms of total phenolic contents (+25%) and antioxidant activities (+20%). Soil fertility and microbial community structure and diversity were also positively affected by the bacterial inoculum. At harvest, the soil with the presence of the bacterial consortium showed an increase in total organic carbon, organic matter, and available phosphorus, as well as higher concentrations of nutrients than the Control. The ecological indexes calculated from the molecular profiles showed that community diversity was positively affected by the bacterial treatment. The present work showed the effective use of plant growth-promoting bacteria as a valid fertilization strategy to improve yield in productive landscapes whilst safeguarding soil biodiversity.

scholarly journals Metagenomic insight into the plant growth-promoting potential of a diesel-degrading bacterial consortium for enhanced rhizoremediation application

2021 ◽  
Author(s):  
Michael O Eze ◽  
Volker Thiel ◽  
Grant C Hose ◽  
Simon C George ◽  
Rolf Daniel
Keyword(s):  
Plant Growth ◽  
Organic Pollutants ◽  
Dna Sequences ◽  
Bacterial Consortium ◽  
Contaminated Site ◽  
Metagenome Analysis ◽  
Remediation Strategies ◽  
Plant Growth Promoting ◽  
Enhanced Bioremediation ◽  
Growth Promoting

The slow rate of natural attenuation of organic pollutants, together with unwanted environmental impacts of traditional remediation strategies, has necessitated the exploration of plant-microbe systems for enhanced bioremediation applications. The identification of microorganisms capable of promoting both plant growth and hydrocarbon degradation is crucial to the success of plant-based remediation techniques. Through successive enrichments of a soil sample from a historic oil-contaminated site in Wietze, Germany, we isolated a plant growth-promoting and hydrocarbon-degrading bacterial consortium. Metagenome analysis of the consortium led to the identification of genes and taxa putatively associated with these processes. The majority of the coding DNA sequences involved in these reactions were affiliated to Acidocella aminolytica and Acidobacterium capsulatum. In microcosm experiments performed in association with Medicago sativa L., the consortium achieved 91% rhizodegradation of diesel fuel hydrocarbons within 60 days, indicating its potential for biotechnological applications in the remediation of sites contaminated by organic pollutants.

scholarly journals Coastal Ecosystems as Sources of Biofertilizers in Agriculture: From Genomics to Application in an Urban Orchard

2021 ◽  
Vol 8 ◽  
Author(s):  
Eloísa Pajuelo ◽  
Sandra Arjona ◽  
Ignacio D. Rodríguez-Llorente ◽  
Enrique Mateos-Naranjo ◽  
Susana Redondo-Gómez ◽  
...  
Keyword(s):  
Plant Growth ◽  
Bacterial Consortium ◽  
Metal Resistance ◽  
Growth And Yield ◽  
Flagellar Motility ◽  
Pilot Experiment ◽  
Metabolic Versatility ◽  
Plant Growth Promoting ◽  
Growth Promoting

Pantoea agglomerans RSO7, a rhizobacterium previously isolated from Spartina maritima grown on metal polluted saltmarshes, had demonstrated good plant growth promoting activity for its host halophyte, but was never tested in crops. The aims of this study were: (1) testing PGP activity on a model plant (alfalfa) in vitro; (2) testing a bacterial consortium including RSO7 as biofertilizer in a pilot experiment in urban orchard; and (3) identifying the traits related to PGP activities. RSO7 was able to enhance alfalfa growth in vitro, particularly the root system, besides improving plant survival and protecting plants against fungal contamination. In addition, in a pilot experiment in urban orchard, a consortium of three bacteria including RSO7 was able to foster the growth and yield of several winter crops between 1.5 and 10 fold, depending on species. Moreover, the analysis of chlorophyll fluorescence revealed that photosynthesis was highly ameliorated. Genome analysis of RSO7 depicted the robustness of this bacterial strain which showed resilience to multiple stresses (heat, cold, UV radiation, several xenobiotics). Together with wide metabolic versatility, genes conferring resistance to oxidative stress were identified. Many genes involved in metal resistance (As, Cu, Ni, Co, Zn, Se, Te) and in tolerance toward high osmolality (production of a battery of osmoprotectans) were also found. Regarding plant growth promoting properties, traits for phosphate solubilization, synthesis of a battery of siderophores and production of IAA were detected. In addition, the bacterium has genes related to key processes in the rhizosphere including flagellar motility, chemotaxis, quorum sensing, biofilm formation, plant-bacteria dialog, and high competitiveness in the rhizosphere. Our results suggest the high potential of this bacterium as bioinoculant for an array of crops. However, the classification in biosecurity group 2 prevents its use according to current European regulation. Alternative formulations for the application of the bioinoculant are discussed.

scholarly journals Bacterial Consortium for Improved Maize (Zea mays L.) Production

2019 ◽  
Vol 7 (11) ◽  
pp. 519 ◽  
Author(s):  
Oluwaseyi Samuel Olanrewaju ◽  
Olubukola Oluranti Babalola
Keyword(s):  
Plant Growth ◽  
Growth Parameters ◽  
Block Design ◽  
Bacterial Consortium ◽  
Microbial Consortia ◽  
Plant Growth Promoting Bacteria ◽  
Promoting Effect ◽  
Maize Seeds ◽  
Plant Growth Promoting ◽  
Growth Promoting

The ever-increasing human population is a major concern for food security. Maize is the third largest most important food crop. The major problems of cultivation arise from urbanization and land pollution. This reduces the amount of land available for agriculture. The use of chemicals in agriculture is not environmentally friendly. Thus, plant growth-promoting bacteria (PGPB) have been proposed as alternatives. This study aims to test the growth-promoting effect of maize inoculated with six indigenous PGPB isolates. These isolates were assayed for various biochemical and plant growth-promoting activities. They were also assayed for biocontrol activities. Based on the results, six isolates viz A1, A18, A29, NWU4, NWU14, and NWU198 were used to inoculate maize seeds. The inoculated seeds were tried out on the field. A randomized block design was used. PGPB used were in single, consortia of two, and three organisms. The length of the leaves, roots, and stem, plant height, numbers of leaves, and weight of 100 seeds were taken at the fourth and eighth weeks after planting. Microbial consortia increased growth parameters compared to single inoculant treatments. Thus, they can be of advantage in the eradication of low yield. They can also serve as reliable alternatives to chemical fertilizers.

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