Preparation of liposomes at large scale using the ethanol injection method: Effect of scale-up and injection devices

In the present study custom screening design was employed to observe the effect of four critical process parameters on particle size and polydispersity index of the liposomal formulation made by ethanol injection method. The four process parameters selected were lipid ratio, rate of injection, phase volume ratio and rotational speed of magnetic stirring. Eight different liposomal formulations were prepared using the design. The formulations were subjected to particle size analysis. The analysis was done at a significance level p<0.05 and found that the process parameters had significant effect on the particle size and polydispersity index of the formulations. The design was optimized for the individual responses with an overall desirability of more than 50%. Three batches of liposomes were formulated at optimized process parameters which matched the target as predicted by the design. Therefore, it can be concluded that the design was effective in production of nano sized stable monodisperse liposomes by ethanol injection method. Dhaka Univ. J. Pharm. Sci. 18(1): 103-111, 2019 (June)

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Ethanol injection method for hydrophilic and lipophilic drug-loaded liposome preparation

Journal of Liposome Research ◽

10.3109/08982100903347923 ◽

2009 ◽

Vol 20 (3) ◽

pp. 228-243 ◽

Cited By ~ 114

Author(s):

Chiraz Jaafar-Maalej ◽

Roudayna Diab ◽

Véronique Andrieu ◽

Abdelhamid Elaissari ◽

Hatem Fessi

Keyword(s):

Ethanol Injection ◽

Lipophilic Drug ◽

Injection Method ◽

Liposome Preparation ◽

Ethanol Injection Method

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Vitamin D3 Loaded Niosomes and Transfersomes Produced by Ethanol Injection Method: Identification of the Critical Preparation Step for Size Control

Foods ◽

10.3390/foods9101367 ◽

2020 ◽

Vol 9 (10) ◽

pp. 1367 ◽

Cited By ~ 1

Author(s):

Oscar R. Estupiñan ◽

Pablo Garcia-Manrique ◽

Maria del Carmen Blanco-Lopez ◽

Maria Matos ◽

Gemma Gutiérrez

Keyword(s):

Vitamin D3 ◽

Scale Up ◽

Size Control ◽

Food Science ◽

Final Size ◽

Ethanol Injection ◽

Vesicle Size ◽

Injection Method ◽

Positive Tendency ◽

Preparation Step

Vesicular nanocarriers have an important role in drug delivery and dietary supplements. Size control and optimization of encapsulation efficiency (EE) should be optimized for those applications. In this work, we report on the identification of the crucial step (injection, evaporation, or sonication) innanovesicles (transfersomes and niosomes) preparation by theethanol injection method (EI). The identification of each production step on the final vesicle size was analyzed in order to optimize further scale-up process. Results indicated that the final size of transfersomeswas clearly influenced by the sonication step while the final size of niosomes was mainly governed by the injection step. Measurements of final surface tension of the different vesicular systems prepared indicate a linear positive tendency with the vesicle size formed. This relation could help to better understand the process and design a vesicular size prediction model for EI. Vitamin D3 (VitD3) was encapsulated in the systems formulated with encapsulation efficiencies larger than 90%. Interaction between the encapsulated compound and the membrane layer components is crucial for vesicle stability. This work has an impact on the scaling-up production of vesicles for further food science applications.

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