One-step purification of α-amylase from the cultivation supernatant of recombinant Bacillus subtilis by high-speed counter-current chromatography with aqueous polymer two-phase systems

2005 ◽  
Vol 1070 (1-2) ◽  
pp. 215-219 ◽  
Author(s):  
Wenbo Zhi ◽  
Qiuyun Deng ◽  
Jiangnan Song ◽  
Ming Gu ◽  
Fan Ouyang
Keyword(s):  
Bacillus Subtilis ◽  
High Speed ◽  
Two Phase ◽  
Aqueous Polymer ◽  
One Step ◽  
Phase Systems ◽  
Recombinant Bacillus Subtilis ◽  
Counter Current

Heterogeneity of hypotonically loaded rat erythrocyte populations as detected by counter-current distribution in aqueous polymer two-phase systems

1996 ◽  
Vol 677 (1) ◽  
pp. 45-51 ◽  
Author(s):  
M. Teresa Pe´rez ◽  
F. Javier Alvarez ◽  
Ana-Isabel Garci´a-Pe´rez ◽  
Luisa Lucas ◽  
M. Cristina Tejedor ◽  
...  
Keyword(s):  
Current Distribution ◽  
Two Phase ◽  
Aqueous Polymer ◽  
Phase Systems ◽  
Counter Current ◽  
Counter Current Distribution

scholarly journals Preparative Separation of Four Major Bufadienolides from the Chinese Traditional Medicine, Chansu, Using High-Speed Counter-Current Chromatography

2010 ◽  
Vol 5 (7) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Xiu Lan Xin ◽  
Junying Liu ◽  
Xiao Chi Ma ◽  
Qing Wei ◽  
Li Lv ◽  
...  
Keyword(s):  
High Speed ◽  
Solvent System ◽  
Two Phase ◽  
Isolation And Purification ◽  
H Nmr ◽  
Chemical Structures ◽  
One Step ◽  
Counter Current ◽  
Chloroform Methanol ◽  
C Nmr

A preparative, high-speed, counter-current chromatographic (HSCCC) method for the isolation and purification of bufadienolides from Chansu was successfully developed by using stepwise elution with a two-phase solvent system composed of n-hexane: chloroform: methanol: water (4:1:2.5:5 and 4:1:4:5, v/v). A total of 7.5 mg of cinobufotalin (1), 8.0 mg of bufalin (2), 14.0 mg of cinobufagin (3) and 9.5 mg of resibufogenin (4) were obtained in a one-step separation from 80 mg of the crude extract with purities of 93.2%, 98.7%, 99.2%, and 99.4%, respectively. The chemical structures were determined from 1H NMR and 13C NMR spectroscopic data.

Biochemical characterization of human erythrocytes fractionated by counter-current distribution in aqueous polymer two-phase systems

1994 ◽  
Vol 668 (1) ◽  
pp. 165-171 ◽  
Author(s):  
Montserrat Pinilla ◽  
Jesus de la Fuente ◽  
Ana I. García-Pérez ◽  
Pilar Jimeno ◽  
Pilar Sancho ◽  
...  
Keyword(s):  
Current Distribution ◽  
Biochemical Characterization ◽  
Human Erythrocytes ◽  
Two Phase ◽  
Aqueous Polymer ◽  
Phase Systems ◽  
Counter Current ◽  
Counter Current Distribution

Spiral coils for counter-current chromatography using aqueous polymer two-phase systems

2007 ◽  
Vol 1151 (1-2) ◽  
pp. 115-120 ◽  
Author(s):  
Y.H. Guan ◽  
J. Smulders ◽  
D. Fisher ◽  
I.A. Sutherland
Keyword(s):  
Two Phase ◽  
Aqueous Polymer ◽  
Phase Systems ◽  
Spiral Coils ◽  
Counter Current

One-step Separation and Purification of Four Phenolic Acids from Stenoloma chusanum (L.) Ching by Medium-pressure Liquid Chromatography and High-speed Counter-current Chromatography

2019 ◽  
Vol 9 (2) ◽  
pp. 138-143
Author(s):  
Tianyun Li ◽  
Xiling Dai ◽  
Yichen Li ◽  
Guozheng Huang ◽  
Jianguo Cao
Keyword(s):  
Natural Products ◽  
Liquid Chromatography ◽  
Phenolic Acids ◽  
High Speed ◽  
High Efficiency ◽  
Total Phenolic ◽  
Medium Pressure ◽  
Medium Pressure Liquid Chromatography ◽  
One Step ◽  
Counter Current

Background:Stenoloma chusanum (L.) Ching is a Chinese traditional medicinal fern with high total flavonoid and total phenolic content. Traditionally, phenolic compounds were separated by using column chromatography, which is relatively inefficient. </P><P> Objective: This study aims to use an efficient method to separate natural products from S. chusanum by Medium-Pressure Liquid Chromatography (MPLC) and High-Speed Counter-Current Chromatography (HSCCC).Methods:In the present research, firstly, a sample (2.5 g) from the dichloromethane extract of S. chusanum was separated by MPLC. Next, fraction P5 was purified by HSCCC with a two-phase solvent system composed of hexane-ethyl acetate-methanol-water (HEMWat) at a volume ratio of 2:4:1:4 (v/v/v/v). </P><P> Result: Four phenolic acids were obtained and their structures were identified by means of NMR and ESI-mass analysis. They were identified as: 1) protocatechuic acid (34 mg, purity 90.1%), 2) syringic acid (66 mg, purity 99.0%), 3) p-hydroxybenzoic acid (5 mg, purity 91.2%) and 4) vanillic acid (6 mg, purity 99.3%).Conclusion:The combination of MPLC and HSCCC is a high-efficiency separation method for natural products. This is the first report with regard to the separation of four phenolic acids in one step by MPLC and HSCCC from S. chusanum (L.) Ching.

Counter-Current Distribution of Proteins in Aqueous Polymer Phase Systems

Nature ◽  
1959 ◽  
Vol 184 (4697) ◽  
pp. 1465-1468 ◽  
Author(s):  
PER-AKE ÅLBERTSSON ◽  
ED. JACQUES NYNS
Keyword(s):  
Current Distribution ◽  
Polymer Phase ◽  
Aqueous Polymer ◽  
Phase Systems ◽  
Counter Current ◽  
Counter Current Distribution
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