Determination of the number of binding sites and binding constant between diltiazem hydrochloride and human serum albumin by ultrasonic microdialysis coupled with online capillary electrophoresis electrochemiluminescence

2010 ◽  
Vol 1217 (28) ◽  
pp. 4753-4756 ◽  
Author(s):  
Biyang Deng ◽  
Hua Lu ◽  
Linqiu Li ◽  
Aihong Shi ◽  
Yanhui Kang ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Binding Sites ◽  
Binding Constant ◽  
Diltiazem Hydrochloride ◽  
Number Of Binding Sites

scholarly journals N-bromosuccinimide-oxidized human serum albumin as a tool for the determination of drug binding sites of human serum albumin.

1983 ◽  
Vol 31 (3) ◽  
pp. 971-978 ◽  
Author(s):  
HIROKO SAKAMOTO ◽  
IZUMI NAGATA ◽  
KIYOMI KIKUCHI ◽  
MASAKO AIDA ◽  
MASACHIKA IRIE
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Binding Sites ◽  
Drug Binding ◽  
Drug Binding Sites

scholarly journals Interaction of the Fungicide Tebuconazole with Human Serum Albumin: A Preliminary Study

2018 ◽  
Vol 62 (2) ◽  
pp. 85-91 ◽  
Author(s):  
J. Staničová ◽  
K. Želonková ◽  
V. Verebová ◽  
B. Holečková ◽  
J. Dianovský
Keyword(s):  
Secondary Structure ◽  
Human Serum Albumin ◽  
Fluorescence Quenching ◽  
Serum Albumin ◽  
Human Serum ◽  
Binding Sites ◽  
Triazole Fungicides ◽  
Number Of Binding Sites ◽  
Preliminary Study ◽  
Protein Macromolecule

Abstract The interactions between the fungicide tebuconazole and human serum albumin were investigated using fluorescence and circular dichroism spectroscopies. The experimental results showed that the fluorescence quenching of the protein by the tebuconazole molecule was a result of the formation of a ligand-protein complex with a binding constant of 8.51×103 l.mol−1 and the number of binding sites in the macromolecule was close to 1. These findings demonstrated the fact that although the binding affinity of tebuconazole to the protein may be slight, it was very similar to other triazole fungicides. In addition, tebuconazole stabilized the α-helical secondary structure of the human serum albumin due to the increase of the α-content in the protein macromolecule.

Spectroscopic and thermodynamic determination of three distinct binding sites for Co(II) ions in human serum albumin☆

2009 ◽  
Vol 103 (7) ◽  
pp. 1005-1013 ◽  
Author(s):  
Magdalena Sokołowska ◽  
Małgorzata Wszelaka-Rylik ◽  
Jarosław Poznański ◽  
Wojciech Bal
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Binding Sites ◽  
Thermodynamic Determination
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