Negative chromatography purification of hepatitis B virus-like particles using poly(oligo(ethylene glycol) methacrylate) grafted cationic adsorbent

2015 ◽  
Vol 1415 ◽  
pp. 161-165 ◽  
Author(s):  
Micky Fu Xiang Lee ◽  
Eng Seng Chan ◽  
Wen Siang Tan ◽  
Kam Chiu Tam ◽  
Beng Ti Tey
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Ethylene Glycol ◽  
Virus Like Particles ◽  
Glycol Methacrylate ◽  
B Virus

scholarly journals Efficient Production of Chimeric Hepatitis B Virus-Like Particles Bearing an Epitope of Hepatitis E Virus Capsid by Transient Expression in Nicotiana benthamiana

Life ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 64
Author(s):  
Gergana Zahmanova ◽  
Milena Mazalovska ◽  
Katerina Takova ◽  
Valentina Toneva ◽  
Ivan Minkov ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Nicotiana Benthamiana ◽  
Hepatitis E Virus ◽  
Chimeric Protein ◽  
Hepatitis E ◽  
Core Antigen ◽  
Efficient Production ◽  
Virus Like Particles ◽  
B Virus

The core antigen of hepatitis B virus (HBcAg) is capable of self-assembly into virus-like particles (VLPs) when expressed in a number of heterologous systems. Such VLPs are potential carriers of foreign antigenic sequences for vaccine design. In this study, we evaluated the production of chimeric HBcAg VLPs presenting a foreign epitope on their surface, the 551–607 amino acids (aa) immunological epitope of the ORF2 capsid protein of hepatitis E virus. A chimeric construct was made by the insertion of 56 aa into the immunodominant loop of the HBcAg. The sequences encoding the chimera were inserted into the pEAQ-HT vector and infiltrated into Nicotiana benthamiana leaves. The plant-expressed chimeric HBcHEV ORF2 551–607 protein was recognized by an anti-HBcAg mAb and anti-HEV IgG positive swine serum. Electron microscopy showed that plant-produced chimeric protein spontaneously assembled into “knobbly” ~34 nm diameter VLPs. This study shows that HBcAg is a promising carrier platform for the neutralizing epitopes of hepatitis E virus (HEV) and the chimeric HBcAg/HEV VLPs could be a candidate for a bivalent vaccine.

Dual-Antigen-Loaded Hepatitis B Virus Core Antigen Virus-like Particles Stimulate Efficient Immunotherapy Against Melanoma

2020 ◽  
Vol 12 (48) ◽  
pp. 53682-53690
Author(s):  
Keman Cheng ◽  
Tao Du ◽  
Yao Li ◽  
Yingqiu Qi ◽  
Huan Min ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Core Antigen ◽  
Virus Like Particles ◽  
Virus Core ◽  
B Virus

Hepatitis B virus-like particles expressing Plasmodium falciparum epitopes induce complement-fixing antibodies against the circumsporozoite protein

Vaccine ◽  
2019 ◽  
Vol 37 (12) ◽  
pp. 1674-1684 ◽  
Author(s):  
Natalie J. Kingston ◽  
Liriye Kurtovic ◽  
Renae Walsh ◽  
Carina Joe ◽  
George Lovrecz ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Circumsporozoite Protein ◽  
Virus Like Particles ◽  
B Virus

scholarly journals Phase I Testing of a Malaria Vaccine Composed of Hepatitis B Virus Core Particles Expressing Plasmodium falciparum Circumsporozoite Epitopes

2004 ◽  
Vol 72 (11) ◽  
pp. 6519-6527 ◽  
Author(s):  
Elizabeth H. Nardin ◽  
Giane A. Oliveira ◽  
J. Mauricio Calvo-Calle ◽  
Kristiane Wetzel ◽  
Carolin Maier ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Phase I ◽  
Malaria Vaccine ◽  
Vaccine Candidate ◽  
Virus Like Particles ◽  
Virus Core ◽  
B Virus ◽  
Malaria Vaccine Candidate

ABSTRACT We report the first phase I trial to assess the safety and immunogenicity of a malaria vaccine candidate, ICC-1132 (Malarivax), composed of a modified hepatitis B virus core protein (HBc) containing minimal epitopes of the Plasmodium falciparum circumsporozoite (CS) protein. When expressed in Escherichia coli, the recombinant ICC-1132 protein forms virus-like particles that were found to be highly immunogenic in preclinical studies of mice and monkeys. Twenty healthy adult volunteers received a 20- or a 50-μg dose of alum-adsorbed ICC-1132 administered intramuscularly at 0, 2, and 6 months. The majority of volunteers in the group receiving the 50-μg dose developed antibodies to CS repeats as well as to HBc. Malaria-specific T cells that secreted gamma interferon were also detected after a single immunization with ICC-1132-alum. These studies support ICC-1132 as a promising malaria vaccine candidate for further clinical testing using more-potent adjuvant formulations and confirm the potential of modified HBc virus-like particles as a delivery platform for vaccines against other human pathogens.

scholarly journals An N-terminal extension to the hepatitis B virus core protein forms a poorly ordered trimeric spike in assembled virus-like particles

2015 ◽  
Vol 189 (2) ◽  
pp. 73-80 ◽  
Author(s):  
Richard McGonigle ◽  
Wei Boon Yap ◽  
Swee Tin Ong ◽  
Derek Gatherer ◽  
Saskia E. Bakker ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Core Protein ◽  
Virus Like Particles ◽  
Virus Core ◽  
B Virus

Observation of virus-like particles in thin sections of the bleaching scleractinian coral Acropora cytherea

2012 ◽  
Vol 93 (4) ◽  
pp. 909-912 ◽  
Author(s):  
Yvan Bettarel ◽  
Nguyen Thanh Thuy ◽  
Tran Quang Huy ◽  
Phan Kim Hoang ◽  
Thierry Bouvier
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Viral Infection ◽  
Protected Area ◽  
Scleractinian Coral ◽  
Marine Protected Area ◽  
Icosahedral Symmetry ◽  
Thin Sections ◽  
Virus Like Particles ◽  
B Virus

Virus-like particles were observed in thin sections of partially bleaching colonies of the scleractinian Acropora cytherea, collected in the Hon Mun Marine Protected Area (Vietnam). The most common particles were found in the cytoplasm of epidermal cells, exhibiting icosahedral symmetry and measuring 90–140 nm in diameter. Some had a tail-like structure resembling that of certain Hepadnaviridae such as the hepatitis B virus. Some others were tailless with a virion size. Overall, less than 10% of the cells showed obvious signs of infection. Images of virally-parasitized tissues of A. cytherea provide further evidence that corals are a target for viral infection. However, more research is required into their pathogenicity and involvement in bleaching events.

scholarly journals An assessment of the use of Hepatitis B Virus core protein virus-like particles to display heterologous antigens from Neisseria meningitidis

Vaccine ◽  
2020 ◽  
Vol 38 (16) ◽  
pp. 3201-3209
Author(s):  
Sebastian Aston-Deaville ◽  
Emil Carlsson ◽  
Muhammad Saleem ◽  
Angela Thistlethwaite ◽  
Hannah Chan ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Neisseria Meningitidis ◽  
Core Protein ◽  
Virus Like Particles ◽  
Virus Core ◽  
B Virus ◽  
Heterologous Antigens

scholarly journals Immunological Analysis of the Hepatitis B Virus “a” Determinant Displayed on Chimeric Virus-Like Particles of Macrobrachium rosenbergii Nodavirus Capsid Protein Produced in Sf9 Cells

Vaccines ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 275
Author(s):  
Nathaniel Nyakaat Ninyio ◽  
Kok Lian Ho ◽  
Hui Kian Ong ◽  
Chean Yeah Yong ◽  
Hui Yee Chee ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Immune Responses ◽  
Natural Killer ◽  
Capsid Protein ◽  
Macrobrachium Rosenbergii ◽  
Hepatitis B Vaccine ◽  
Chimeric Virus ◽  
Virus Like Particles ◽  
B Virus

Chimeric virus-like particles (VLPs) have been widely exploited for various purposes including their use as vaccine candidates, particularly due to their ability to induce stronger immune responses than VLPs consisting of single viral proteins. In the present study, VLPs of the Macrobrachium rosenbergii nodavirus (MrNV) capsid protein (Nc) displaying the hepatitis B virus “a” determinant (aD) were produced in Spodoptera frugiperda (Sf9) insect cells. BALB/c mice immunised with the purified chimeric Nc-aD VLPs elicited a sustained titre of anti-aD antibody, which was significantly higher than that elicited by a commercially available hepatitis B vaccine and Escherichia coli-produced Nc-aD VLPs. Immunophenotyping showed that the Sf9-produced Nc-aD VLPs induced proliferation of cytotoxic T-lymphocytes and NK1.1 natural killer cells. Furthermore, enzyme-linked immunospot (ELISPOT)analysis showed the presence of antibody-secreting memory B cells in the mice splenocytes stimulated with the synthetic aD peptide. The significant humoral, natural killer cell and memory B cell immune responses induced by the Sf9-produced Nc-aD VLPs suggest that they present good prospects for use as a hepatitis B vaccine candidate.

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