Determination of ciprofloxacin and levofloxacin in human sputum collected from cystic fibrosis patients using microextraction by packed sorbent-high performance liquid chromatography photodiode array detector

2015 ◽  
Vol 1419 ◽  
pp. 58-66 ◽  
Author(s):  
Marcello Locatelli ◽  
Maria Teresa Ciavarella ◽  
Donatella Paolino ◽  
Christian Celia ◽  
Ersilia Fiscarelli ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Array Detector ◽  
Microextraction By Packed Sorbent ◽  
Photodiode Array Detector ◽  
Photodiode Array ◽  
Packed Sorbent

Determination of monofluorophosphate, orthophosphate, and polyphosphates by high-performance liquid chromatography with a photodiode array detector

1992 ◽  
Vol 64 (13) ◽  
pp. 1499-1501 ◽  
Author(s):  
Norimasa. Yoza ◽  
Sachiko. Nakashima ◽  
Tetsuya. Nakazato ◽  
Nobuyuki. Ueda ◽  
Hiroki. Kodama ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Array Detector ◽  
Photodiode Array Detector ◽  
Photodiode Array

scholarly journals Development and validation of indocyanine green assay in human plasma using High-Performance Liquid Chromatography with PDA detector

2019 ◽  
Vol 10 (2) ◽  
pp. 1007-1012
Author(s):  
Sumith K Mathew ◽  
Blessed Winston A ◽  
Aswathy Mathew ◽  
Jeana Jacob ◽  
Ratna Prabha ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Indocyanine Green ◽  
High Performance ◽  
Array Detector ◽  
Photodiode Array Detector ◽  
Photodiode Array ◽  
Blood Specimens ◽  
Development And Validation

A robust and economical assay for routine determination of indocyanine green pharmacokinetics was developed and validated using high-performance liquid chromatography with a photodiode array detector. Plasma specimens from critically ill patients and those with hepatitis on various co-medications were used as blanks for validation of this assay. Extraction of indocyanine green was performed by simple protein precipitation with acetonitrile, and the supernatant was separated using an octadecyl column with detection at 784 nm. Blanks were found to have no interference for 40 blanks of patients who were on 56 different medications. The precision for LLOQ (0.5 µg/ml) as determined by the percentage coefficient of variation was 1.19. Stability of plasma calibration standards and stock were determined over a period of 61 days, and ICG was found to be stable for 20 days. Stability of whole blood specimens containing ICG was determined at 4°C for a period of 4 hours.

scholarly journals DETERMINATION OF LUTEOLIN FROM EXTRACTIONS OF Helicteres hirsuta BY HPLC

2019 ◽  
Vol 128 (1B) ◽  
pp. 43
Author(s):  
Lê Trung Hiếu ◽  
Lê Lâm Sơn ◽  
Nguyễn Minh Nhung ◽  
Hồ Xuân Anh Vũ ◽  
Trần Thị Văn Thi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Array Detector ◽  
Plant Parts ◽  
Aerial Parts ◽  
Photodiode Array Detector ◽  
Photodiode Array

<p>High performance liquid chromatography coupled with photodiode array detector (HPLC- DAD) has been reported to quantify isolated compounds. This work was designed, therefore, to develop an HPLC-DAD system to determine luteolin in extractive solutions from <em>Helicteres hirsuta</em>. Luteolin was analyzed on a RP- C18 column, using a mobile phase including: acetonitrile - 0.1% acid phosphoric (v/v), under the following conditions detecting wavelength: 347 nm; flow rate: 0.5 mL/min, and the volume of injecting sample: 10 μL. The HPLC system was carried out at ambient temperature. The method showed linearity for luteolin in the range to 0.02 from 1 mg/mL, and the recovery of luteolin was 94.07 ± 0.64. Contents of  luteolin in methanol extracts from the plant parts of <em>H. hirsuta</em> (including: branch, leaf, fruit and aerial parts) were determined with value of 49.06 ±0.46, 142.89 ±0.53, 56.61±0.62 and 91.15±0.42 µg/g, respectively.</p>

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