Quasi-adiabatic vacuum-based column housing for very high-pressure liquid chromatography

2016 ◽  
Vol 1456 ◽  
pp. 226-234 ◽  
Author(s):  
Fabrice Gritti ◽  
Martin Gilar ◽  
Joseph A. Jarrell
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Very High ◽  
Adiabatic Vacuum

scholarly journals Ultra High Pressure Liquid Chromatography for Crude Plant Extract Profiling

2011 ◽  
Vol 94 (1) ◽  
pp. 51-70 ◽  
Author(s):  
Philippe J Eugster ◽  
Davy Guillarme ◽  
Serge Rudaz ◽  
Jean-Luc Veuthey ◽  
Pierre-Alain Carrupt ◽  
...  
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
High Pressures ◽  
Product Analysis ◽  
Basic Principles ◽  
Ultra High Pressure ◽  
Crude Plant Extract ◽  
Phase Chemistry ◽  
Very High

Abstract Ultra high pressure liquid chromatography (UHPLC) systems operating at very high pressures and using sub-2 μm packing columns have allowed a remarkable decrease in analysis time and increase in peak capacity, sensitivity, and reproducibility compared to conventional HPLC. This technology has rapidly been widely accepted by the analytical community and is being gradually applied to various fields of plant analysis such as QC, profiling and fingerprinting, dereplication, and metabolomics. For many applications, an important improvement of the overall performances has been reported. In this review, the basic principles of UHPLC are summarized, and practical information on the type of columns used and phase chemistry available is provided. An overview of the latest applications to natural product analysis in complex mixtures is given, and the potential and limitations as well as some new trends in the development of UHPLC are discussed.

Immunoreactive vasopressin and oxytocin in hypothalamo-hypophysial portal blood of the Brattleboro and Long–Evans rat: effect of adrenalectomy and dexamethasone

1988 ◽  
Vol 117 (1) ◽  
pp. 27-34 ◽  
Author(s):  
D. J. A. Eckland ◽  
K. Todd ◽  
S. L. Lightman
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Portal Blood ◽  
Brattleboro Rats ◽  
Brattleboro Rat ◽  
Long Evans ◽  
High Concentrations ◽  
Very High ◽  
Hypophysial Portal

ABSTRACT Immunoreactive vasopressin and oxytocin were measured in the hypothalamo-hypophysial portal blood of both Long–Evans and homozygous Brattleboro rats. Adrenalectomy caused an increase in vasopressin immunoreactivity in portal blood in the Long–Evans strain, whilst administration of dexamethasone to these adrenalectomized animals resulted in a reduction in portal vasopressin immunoreactivity to levels below those seen in sham-operated animals. This vasopressin immunoreactivity co-eluted with synthetic vasopressin on high-pressure liquid chromatography (HPLC), and diluted in parallel in radioimmunoassay. In Brattleboro rats, however, although vasopressin-like immunoreactivity was detected, the portal concentration did not vary with the adrenal status of the animal, nor did it show the characteristics of standard vasopressin on HPLC or in immunoassay. Oxytocin was present in the portal blood of both Long–Evans and Brattleboro rats at similar very high concentrations, but did not vary in response to adrenalectomy. These results are consistent with a role for vasopressin, but not oxytocin, in the hypothalamic response to adrenalectomy and glucocorticoid feedback. Neither vasopressin immunoreactivity nor oxytocin appear to subserve this role in the homozygous Brattleboro rat. J. Endocr. (1988) 117, 27–34

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