Practical limits to column performance in liquid chromatography – Optimal operations

2020 ◽  
Vol 1629 ◽  
pp. 461482
Author(s):  
Leonid M. Blumberg
Keyword(s):  
Liquid Chromatography ◽  
Column Performance

High-performance liquid chromatography of quinidine in plasma, with use of a microparticulate silica column.

1978 ◽  
Vol 24 (12) ◽  
pp. 2166-2168 ◽  
Author(s):  
M A Peat ◽  
T A Jennison
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Correlation Coefficient ◽  
High Performance ◽  
Plasma Concentrations ◽  
Internal Standard ◽  
Standard Addition ◽  
Routine Method ◽  
Silica Column ◽  
Column Performance

Abstract We describe a routine method for determining plasma concentrations of quinidine by liquid chromatography. The procedure requires 1.0 ml of plasma (or serum) and involves internal standard addition, extraction with ether, and separation on a column of microparticulate silica. Day-to-day CV (15 days) was less than 5% and no deterioration in column performance has been observed during 12 months. Comparison with a fluorometric procedure gave a correlation coefficient of 0.995.

Protocol to compare column performance applied to hydrophilic interaction liquid chromatography

2019 ◽  
Vol 149 ◽  
pp. 103973 ◽  
Author(s):  
R. Burgos-Gil ◽  
E. Peris-García ◽  
M.J. Ruiz-Angel ◽  
J.J. Baeza-Baeza ◽  
M.C. García-Alvarez-Coque
Keyword(s):  
Liquid Chromatography ◽  
Hydrophilic Interaction Liquid Chromatography ◽  
Hydrophilic Interaction ◽  
Column Performance
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