Enhanced cold active lipase production by metagenomic library recombinant clone CALIP3 with a step-wise temperature and dissolved oxygen level control strategy

2016 ◽  
Vol 24 (9) ◽  
pp. 1263-1269 ◽  
Author(s):  
Zhuhua Chan ◽  
Runping Wang ◽  
Fan Yang ◽  
Runying Zeng
Keyword(s):  
Dissolved Oxygen ◽  
Control Strategy ◽  
Metagenomic Library ◽  
Lipase Production ◽  
Oxygen Level ◽  
Level Control ◽  
Recombinant Clone ◽  
Cold Active ◽  
Active Lipase

scholarly journals Optimization of Cold-Active Lipase Production from Psychrophilic BacteriumMoritellasp. 2-5-10-1 by Statistical Experimental Methods

2013 ◽  
Vol 77 (1) ◽  
pp. 17-21 ◽  
Author(s):  
Quanfu WANG ◽  
Chunyu ZHANG ◽  
Yanhua HOU ◽  
Xuezheng LIN ◽  
Jihong SHEN ◽  
...  
Keyword(s):  
Experimental Methods ◽  
Lipase Production ◽  
Cold Active ◽  
Active Lipase

scholarly journals Production of Cold-Active Bacterial Lipases through Semisolid State Fermentation Using Oil Cakes

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Babu Joseph ◽  
Supriya Upadhyaya ◽  
Pramod Ramteke
Keyword(s):  
Large Scale ◽  
Lipase Production ◽  
Maximum Activity ◽  
Mustard Oil ◽  
Western Himalayas ◽  
Semisolid State ◽  
Groundnut Oil ◽  
Enhanced Production ◽  
Cold Active ◽  
Active Lipase

Production of cold active lipase by semisolid state fermentation involves the use of agroindustrial residues. In the present study, semisolid state fermentation was carried out for the production of cold active lipase using Micrococcus roseus, isolated from soil samples of Gangotri glaciers, Western Himalayas. Among various substrate tested, groundnut oil cake (GOC) favored maximal yield of lipases at 15 ± 1°C within 48 h. Supplementation of glucose 1% (w/v) as additional carbon source and ammonium nitrate 2% (w/v) as additional nitrogen source enhanced production of lipase. Addition of triglycerides 0.5% (v/v) tends to repress the lipase production. Further mixed preparation of groundnut oil cake (GOC) along with mustard oil cake (MOC) in the ratio of 1 : 1, and its optimization resulted in improved production of cold active lipase. The enzyme exhibited maximum activity at 10–15°C and was stable at temperatures lower than 30°C. The lipase exhibited optimum activity at pH 8 and showed more than 60% stability at pH 9. Semisolid state fermentation process by utilizing agroindustrial wastes will direct to large-scale commercialization of lipase catalyzed process in cost-effective systems.

Characterization of a cold-active lipase from Psychrobacter cryohalolentis K5T and its deletion mutants

2013 ◽  
Vol 78 (4) ◽  
pp. 385-394 ◽  
Author(s):  
K. A. Novototskaya-Vlasova ◽  
L. E. Petrovskaya ◽  
E. M. Rivkina ◽  
D. A. Dolgikh ◽  
M. P. Kirpichnikov
Keyword(s):  
Deletion Mutants ◽  
Cold Active ◽  
Active Lipase

scholarly journals Identification and Characterization of a Halotolerant, Cold-Active Marine Endo-β-1,4-Glucanase by Using Functional Metagenomics of Seaweed-Associated Microbiota

2014 ◽  
Vol 80 (16) ◽  
pp. 4958-4967 ◽  
Author(s):  
Marjolaine Martin ◽  
Sophie Biver ◽  
Sébastien Steels ◽  
Tristan Barbeyron ◽  
Murielle Jam ◽  
...  
Keyword(s):  
Metagenomic Library ◽  
Functional Screening ◽  
Sample Collection ◽  
Prolonged Incubation ◽  
Content Type ◽  
Cold Active ◽  
Esterase Loci ◽  
Collection Period ◽  
Identification And Characterization

ABSTRACTA metagenomic library was constructed from microorganisms associated with the brown algaAscophyllum nodosum. Functional screening of this library revealed 13 novel putative esterase loci and two glycoside hydrolase loci. Sequence and gene cluster analysis showed the wide diversity of the identified enzymes and gave an idea of the microbial populations present during the sample collection period. Lastly, an endo-β-1,4-glucanase having less than 50% identity to sequences of known cellulases was purified and partially characterized, showing activity at low temperature and after prolonged incubation in concentrated salt solutions.

DC-Bus Dual-Level Control Strategy for PV Power System With Dual-Mode Operation

2019 ◽  
Vol 34 (1) ◽  
pp. 267-276 ◽  
Author(s):  
Terng-Wei Tsai ◽  
Cheng-Jhen Yang ◽  
Yi-Chan Li ◽  
Yaow-Ming Chen ◽  
Yung-Ruei Chang
Keyword(s):  
Power System ◽  
Control Strategy ◽  
Level Control ◽  
Dual Mode ◽  
Mode Operation ◽  
Dc Bus

Rule based control of a periodic activated sludge process

1998 ◽  
Vol 38 (3) ◽  
pp. 281-289 ◽  
Author(s):  
S. Isaacs ◽  
D. Thornberg
Keyword(s):  
Dissolved Oxygen ◽  
Activated Sludge ◽  
Control Strategy ◽  
Periodic Operation ◽  
Rule Based ◽  
Criterion Functions ◽  
Strategy Performance ◽  
Working Region ◽  
And Control ◽  
Selection Of

A rule based control strategy for automatically adjusting phase lengths and aeration intensity for an activated sludge nutrient removal process based on a periodic operation is examined using simulations based on the Activated Sludge Model No. 1. The strategy is based on four criterion functions, two which determine the switching of the roles of two nitrifying/denitrifying reactors and two which adjust the dissolved oxygen setpoint levels in the two reactors as functions of ammonia and nitrate concentrations. Trajectory plots of reactor concentrations in the ammonia-nitrate plane are shown to be a useful means of visualizing process and control performance. Together, the trajectories from a working region in the ammonia-nitrate plane, the size and location of which can to some extent be predetermined by selection of the criterion functions. The presented results include the influence of one of the criterion functions on control strategy performance, how an incompatibility between two criterion functions can lead to unsymmetric reactor loading, and the effect of allowing simultaneous nitrification and denitrification during nitrifying periods by reducing the dissolved oxygen level as ammonia is consumed.

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