scholarly journals Production of Cold-Active Bacterial Lipases through Semisolid State Fermentation Using Oil Cakes

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Babu Joseph ◽  
Supriya Upadhyaya ◽  
Pramod Ramteke
Keyword(s):  
Large Scale ◽  
Lipase Production ◽  
Maximum Activity ◽  
Mustard Oil ◽  
Western Himalayas ◽  
Semisolid State ◽  
Groundnut Oil ◽  
Enhanced Production ◽  
Cold Active ◽  
Active Lipase

Production of cold active lipase by semisolid state fermentation involves the use of agroindustrial residues. In the present study, semisolid state fermentation was carried out for the production of cold active lipase using Micrococcus roseus, isolated from soil samples of Gangotri glaciers, Western Himalayas. Among various substrate tested, groundnut oil cake (GOC) favored maximal yield of lipases at 15 ± 1°C within 48 h. Supplementation of glucose 1% (w/v) as additional carbon source and ammonium nitrate 2% (w/v) as additional nitrogen source enhanced production of lipase. Addition of triglycerides 0.5% (v/v) tends to repress the lipase production. Further mixed preparation of groundnut oil cake (GOC) along with mustard oil cake (MOC) in the ratio of 1 : 1, and its optimization resulted in improved production of cold active lipase. The enzyme exhibited maximum activity at 10–15°C and was stable at temperatures lower than 30°C. The lipase exhibited optimum activity at pH 8 and showed more than 60% stability at pH 9. Semisolid state fermentation process by utilizing agroindustrial wastes will direct to large-scale commercialization of lipase catalyzed process in cost-effective systems.

scholarly journals Optimization of Cold-Active Lipase Production from Psychrophilic BacteriumMoritellasp. 2-5-10-1 by Statistical Experimental Methods

2013 ◽  
Vol 77 (1) ◽  
pp. 17-21 ◽  
Author(s):  
Quanfu WANG ◽  
Chunyu ZHANG ◽  
Yanhua HOU ◽  
Xuezheng LIN ◽  
Jihong SHEN ◽  
...  
Keyword(s):  
Experimental Methods ◽  
Lipase Production ◽  
Cold Active ◽  
Active Lipase

Characterization of a cold-active lipase from Psychrobacter cryohalolentis K5T and its deletion mutants

2013 ◽  
Vol 78 (4) ◽  
pp. 385-394 ◽  
Author(s):  
K. A. Novototskaya-Vlasova ◽  
L. E. Petrovskaya ◽  
E. M. Rivkina ◽  
D. A. Dolgikh ◽  
M. P. Kirpichnikov
Keyword(s):  
Deletion Mutants ◽  
Cold Active ◽  
Active Lipase

scholarly journals Enhanced production of 99Mo in inverse kinematics heavy ion reactions

2021 ◽  
Vol 252 ◽  
pp. 08003
Author(s):  
Justin Mabiala ◽  
Marcia R.D. Rodrigues ◽  
Georgios A. Souliotis ◽  
Victor E. Iacob ◽  
Ninel Nica ◽  
...  
Keyword(s):  
Inverse Kinematics ◽  
Large Scale ◽  
Heavy Ion ◽  
Scale Production ◽  
Gas Cell ◽  
Enhanced Production ◽  
Gas Target ◽  
Large Scale Production ◽  
Cell Target ◽  
Catcher Foil

The reaction of a 100Mo beam at 12 MeV/nucleon impinging on a 4He gas-cell target was performed. The 99Mo alongside other coproduced isotopes were collected after the gas target on an aluminum catcher foil and their respective radioactivities were measured by offline γ-ray analysis. In this contribution, preliminary experimental results which are used to discuss the possibility of optimal large-scale production conditions of the produced radioisotopes are presented.

Enhanced production of falcarinol-type polyacetylenes in hairy roots of cultivated carrot (Daucus carota subsp. sativus)

2021 ◽  
Author(s):  
Frank Dunemann ◽  
Christoph Böttcher
Keyword(s):  
Hairy Root ◽  
Large Scale ◽  
Higher Plants ◽  
Leaf Tissue ◽  
Hairy Root Cultures ◽  
Scale Production ◽  
Root Cultures ◽  
Tissue Samples ◽  
Positive Effects ◽  
Enhanced Production

Abstract Polyacetylenes (PAs) are a large group of bioactive phytochemicals, which are primarily produced by higher plants of the families Apiaceae and Araliaceae. Especially aliphatic C17-polyacetylenes of the falcarinol-type such as falcarinol (FaOH) and falcarindiol (FaDOH) are known for their numerous positive effects on human health. In this study we investigate the potential of carrot hairy root cultures for production of PAs. Three individual plants of seven differently coloured carrot cultivars were used for the development of hairy root cultures by transformation of root discs with the wild-type Rhizobium rhizogenes strain 15834. A total of 51 individual hairy root (HR) lines were obtained and quantitatively analysed together with root, petiole and leaf tissue samples for FaOH and FaDOH. Among the five tissues sampled from the donor plants, root periderm samples generally exhibited the highest PA levels with FaDOH as prevailing PA and large differences between cultivars. In comparison to periderm tissue, FaOH levels were highly increased in HR lines of all cultivars. In contrast, FaDOH levels were not significantly altered. Considering the low to moderate PA concentration in root and leaf tissues of the orange cultivars there was an up to more than 10-fold increase of the FaOH concentration in HRs of these genotypes. Within this study a reproducible method for Rrhi-mediated transformation of carrot root discs was applied which provides an efficient tool to assess the function of candidate genes involved in the biosynthesis of key PAs in carrot but might be used in future also for the large-scale production of falcarinol-type PAs.

Oxidative Stress, Mitochondrial DNA Mutation, and Impairment of Antioxidant Enzymes in Aging

2002 ◽  
Vol 227 (9) ◽  
pp. 671-682 ◽  
Author(s):  
Yau-Huei Wei ◽  
Hsin-Chen Lee
Keyword(s):  
Oxidative Stress ◽  
Mitochondrial Dna ◽  
Antioxidant Enzymes ◽  
Oxidative Damage ◽  
Large Scale ◽  
Wide Spectrum ◽  
Radical Scavenging ◽  
Mtdna Mutations ◽  
Enhanced Production ◽  
Age Related

Mitochondria do not only produce less ATP, but they also increase the production of reactive oxygen species (ROS) as byproducts of aerobic metabolism in the aging tissues of the human and animals. It is now generally accepted that aging-associated respiratory function decline can result in enhanced production of ROS in mitochondria. Moreover, the activities of free radical-scavenging enzymes are altered in the aging process. The concurrent age-related changes of these two systems result in the elevation of oxidative stress in aging tissues. Within a certain concentration range, ROS may induce stress response of the cells by altering expression of respiratory genes to uphold the energy metabolism to rescue the cell. However, beyond the threshold, ROS may cause a wide spectrum of oxidative damage to various cellular components to result in cell death or elicit apoptosis by induction of mitochondrial membrane permeability transition and release of apoptogenic factors such as cytochrome c. Moreover, oxidative damage and large-scale deletion and duplication of mitochondrial DNA (mtDNA) have been found to increase with age in various tissues of the human. Mitochondria act like a biosensor of oxidative stress and they enable cell to undergo changes in aging and age-related diseases. On the other hand, it has recently been demonstrated that impairment in mitochondrial respiration and oxidative phosphorylation elicits an increase in oxidative stress and causes a host of mtDNA rearrangements and deletions. Here, we review work done in the past few years to support our view that oxidative stress and oxidative damage are a result of concurrent accumulation of mtDNA mutations and defective antioxidant enzymes in human aging.

scholarly journals Transcriptional and Small RNA Responses of the White Mold Fungus Sclerotinia sclerotiorum to Infection by a Virulence-Attenuating Hypovirus

Viruses ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 713 ◽  
Author(s):  
Shin-Yi Lee Marzano ◽  
Achal Neupane ◽  
Leslie Domier
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Small Rna ◽  
Small Rnas ◽  
Large Scale ◽  
White Mold ◽  
Enhanced Production ◽  
Mold Fungus ◽  
Depth Analysis ◽  
Sclerotial Formation ◽  
In Trans

Mycoviruses belonging to the family Hypoviridae cause persistent infection of many different host fungi. We previously determined that the white mold fungus, Sclerotinia sclerotiorum, infected with Sclerotinia sclerotiorum hypovirus 2-L (SsHV2-L) exhibits reduced virulence, delayed/reduced sclerotial formation, and enhanced production of aerial mycelia. To gain better insight into the cellular basis for these changes, we characterized changes in mRNA and small RNA (sRNA) accumulation in S. sclerotiorum to infection by SsHV2-L. A total of 958 mRNAs and 835 sRNA-producing loci were altered after infection by SsHV2-L, among which >100 mRNAs were predicted to encode proteins involved in the metabolism and trafficking of carbohydrates and lipids. Both S. sclerotiorum endogenous and virus-derived sRNAs were predominantly 22 nt in length suggesting one dicer-like enzyme cleaves both. Novel classes of endogenous small RNAs were predicted, including phasiRNAs and tRNA-derived small RNAs. Moreover, S. sclerotiorum phasiRNAs, which were derived from noncoding RNAs and have the potential to regulate mRNA abundance in trans, showed differential accumulation due to virus infection. tRNA fragments did not accumulate differentially after hypovirus infection. Hence, in-depth analysis showed that infection of S. sclerotiorum by a hypovirulence-inducing hypovirus produced selective, large-scale reprogramming of mRNA and sRNA production.

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