Analysis of interaction between glutamic acid and ninhydrin in the presence of acetate buffer solvent: Impact of gemini (twin-headed) surfactants

1. Peptides and α-amino acids, occurring in mixtures from various sources, can be separated into one fraction containing the amino acids and several peptide fractions. This is achieved by chelation of the mixture with Cu2+ ions and subsequent chromatography of these chelates over the acetate form of diethylaminoethylcellulose or triethylaminoethylcellulose. 2. The amino acid fraction is obtained by elution with 0·01m-collidine–acetate buffer, pH8·0. 3. Peptide fractions are eluted with 0·01m-collidine–acetate buffer, pH4·5, 0·17n-acetic acid and 0·1n-hydrochloric acid respectively. 4. With the exception of aspartic acid and glutamic acid, which are partly found in the acidic peptide fraction, the amino acids are completely separated from the peptides. 5. Contamination of the acidic peptide fraction with glutamic acid and aspartic acid can be largely avoided by previous addition of an excess of arginine. 6. Copper is removed from the eluates by extraction with 8-hydroxyquinoline in chloroform.

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DETERMINATION OF GLUTAMINE AND GLUTAMIC ACID IN THE BLOOD PLASMA OF CHICKS BY A COMBINED CHROMATOGRAPHIC AND MICROBIOLOGICAL METHOD

Canadian Journal of Biochemistry and Physiology ◽

10.1139/y62-042 ◽

1962 ◽

Vol 40 (1) ◽

pp. 381-390 ◽

Cited By ~ 3

Author(s):

Ellen M. Olsen ◽

D. C. Hill ◽

H. D. Branion

Keyword(s):

Glutamic Acid ◽

Blood Plasma ◽

Lactobacillus Plantarum ◽

Test Organism ◽

Microbiological Assay ◽

Acetate Buffer ◽

Microbiological Method ◽

Chromatographic Procedure

Glutamine was separated from glutamic acid by chromatographing deproteinized plasma on a 10-cm column of Dowex 1X8 and by eluting with acetate buffer, pH 4.2. The first portion of eluate, which contained the glutamine but no glutamic acid, was subjected to microbiological assay using Lactobacillus plantarum as a test organism. Recovery of glutamine added to plasma was satisfactory and the precision of the assays conducted was within 4%. Several substances were found to interfere with the microbiological assay but these were eliminated by the chromatographic procedure. The method was extended to the determination of glutamic acid by continued elution of the column with acetate buffer, pH 4.2, fractionation of the eluate, and determination with ninhydrin. Average glutamine and glutamic acid contents of plasma of normal non-fasting chickens were 12.8 and 3.4 mg per 100 ml respectively.

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DETERMINATION OF GLUTAMINE AND GLUTAMIC ACID IN THE BLOOD PLASMA OF CHICKS BY A COMBINED CHROMATOGRAPHIC AND MICROBIOLOGICAL METHOD

Canadian Journal of Biochemistry and Physiology ◽

10.1139/o62-042 ◽

1962 ◽

Vol 40 (3) ◽

pp. 381-390 ◽

Cited By ~ 1

Author(s):

Ellen M. Olsen ◽

D. C. Hill ◽

H. D. Branion

Keyword(s):

Glutamic Acid ◽

Blood Plasma ◽

Lactobacillus Plantarum ◽

Test Organism ◽

Microbiological Assay ◽

Acetate Buffer ◽

Microbiological Method ◽

Chromatographic Procedure

Glutamine was separated from glutamic acid by chromatographing deproteinized plasma on a 10-cm column of Dowex 1X8 and by eluting with acetate buffer, pH 4.2. The first portion of eluate, which contained the glutamine but no glutamic acid, was subjected to microbiological assay using Lactobacillus plantarum as a test organism. Recovery of glutamine added to plasma was satisfactory and the precision of the assays conducted was within 4%. Several substances were found to interfere with the microbiological assay but these were eliminated by the chromatographic procedure. The method was extended to the determination of glutamic acid by continued elution of the column with acetate buffer, pH 4.2, fractionation of the eluate, and determination with ninhydrin. Average glutamine and glutamic acid contents of plasma of normal non-fasting chickens were 12.8 and 3.4 mg per 100 ml respectively.

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The role of silicon in forages: A quantitative X-Ray study

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100126871 ◽

1987 ◽

Vol 45 ◽

pp. 416-417

Author(s):

Janet H. Woodward ◽

D. E. Akin

Keyword(s):

Sodium Acetate ◽

Dry Matter ◽

Acetate Buffer ◽

Plant Tissues ◽

Sodium Acetate Buffer ◽

X Ray ◽

Dry Matter Digestibility ◽

Percent Composition

Silicon (Si) is distributed throughout plant tissues, but its role in forages has not been clarified. Although Si has been suggested as an antiquality factor which limits the digestibility of structural carbohydrates, other research indicates that its presence in plants does not affect digestibility. We employed x-ray microanalysis to evaluate Si as an antiquality factor at specific sites of two cultivars of bermuda grass (Cynodon dactvlon (L.) Pers.). “Coastal” and “Tifton-78” were chosen for this study because previous work in our lab has shown that, although these two grasses are similar ultrastructurally, they differ in in vitro dry matter digestibility and in percent composition of Si.Two millimeter leaf sections of Tifton-7 8 (Tift-7 8) and Coastal (CBG) were incubated for 72 hr in 2.5% (w/v) cellulase in 0.05 M sodium acetate buffer, pH 5.0. For controls, sections were incubated in the sodium acetate buffer or were not treated.

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