DETERMINATION OF GLUTAMINE AND GLUTAMIC ACID IN THE BLOOD PLASMA OF CHICKS BY A COMBINED CHROMATOGRAPHIC AND MICROBIOLOGICAL METHOD

1962 ◽  
Vol 40 (1) ◽  
pp. 381-390 ◽  
Author(s):  
Ellen M. Olsen ◽  
D. C. Hill ◽  
H. D. Branion
Keyword(s):  
Glutamic Acid ◽  
Blood Plasma ◽  
Lactobacillus Plantarum ◽  
Test Organism ◽  
Microbiological Assay ◽  
Acetate Buffer ◽  
Microbiological Method ◽  
Chromatographic Procedure

Glutamine was separated from glutamic acid by chromatographing deproteinized plasma on a 10-cm column of Dowex 1X8 and by eluting with acetate buffer, pH 4.2. The first portion of eluate, which contained the glutamine but no glutamic acid, was subjected to microbiological assay using Lactobacillus plantarum as a test organism. Recovery of glutamine added to plasma was satisfactory and the precision of the assays conducted was within 4%. Several substances were found to interfere with the microbiological assay but these were eliminated by the chromatographic procedure. The method was extended to the determination of glutamic acid by continued elution of the column with acetate buffer, pH 4.2, fractionation of the eluate, and determination with ninhydrin. Average glutamine and glutamic acid contents of plasma of normal non-fasting chickens were 12.8 and 3.4 mg per 100 ml respectively.

DETERMINATION OF GLUTAMINE AND GLUTAMIC ACID IN THE BLOOD PLASMA OF CHICKS BY A COMBINED CHROMATOGRAPHIC AND MICROBIOLOGICAL METHOD

1962 ◽  
Vol 40 (3) ◽  
pp. 381-390 ◽  
Author(s):  
Ellen M. Olsen ◽  
D. C. Hill ◽  
H. D. Branion
Keyword(s):  
Glutamic Acid ◽  
Blood Plasma ◽  
Lactobacillus Plantarum ◽  
Test Organism ◽  
Microbiological Assay ◽  
Acetate Buffer ◽  
Microbiological Method ◽  
Chromatographic Procedure

Glutamine was separated from glutamic acid by chromatographing deproteinized plasma on a 10-cm column of Dowex 1X8 and by eluting with acetate buffer, pH 4.2. The first portion of eluate, which contained the glutamine but no glutamic acid, was subjected to microbiological assay using Lactobacillus plantarum as a test organism. Recovery of glutamine added to plasma was satisfactory and the precision of the assays conducted was within 4%. Several substances were found to interfere with the microbiological assay but these were eliminated by the chromatographic procedure. The method was extended to the determination of glutamic acid by continued elution of the column with acetate buffer, pH 4.2, fractionation of the eluate, and determination with ninhydrin. Average glutamine and glutamic acid contents of plasma of normal non-fasting chickens were 12.8 and 3.4 mg per 100 ml respectively.

Improved Cleanup and Derivatization for Gas Chromatographic Determination of Monosodium Glutamate in Foods

1983 ◽  
Vol 66 (6) ◽  
pp. 1528-1531 ◽  
Author(s):  
Hiroshi Nakanishi
Keyword(s):  
Glutamic Acid ◽  
Acid Derivative ◽  
Monosodium Glutamate ◽  
Chromatographic Determination ◽  
Flame Ionization Detection ◽  
Gas Chromatograph ◽  
Flame Ionization ◽  
Acetone Water ◽  
Chromatographic Procedure

Abstract A gas chromatographic procedure is described for determining monosodium glutamate (MSG) in several types of food. A sample is extracted with acetone- water (1 + 1). Acetone is evaporated and an aliquot of the extract is buffered with 1M NH4OH-1M NH4CI pH 9 solution, and chromatographed directly on a column of QAE Sephadex A-25 that has been pretreated with the same buffer. MSG is eluted with 0.1N HC1, and a portion of the eluate is evaporated to dryness and reacted with dimethylformamide( DMF)-dimethylacetal to form the glutamic acid derivative, which is injected into a gas chromatograph and measured by flame ionization detection. Recoveries of MSG from sample fortified at 5-500 mg ranged from 92.8 to 100%.

A MICROBIOLOGICAL ASSAY FOR THE QUANTITATIVE DETERMINATION OF GLYCEROL

1960 ◽  
Vol 6 (3) ◽  
pp. 283-287 ◽  
Author(s):  
L. Eidus ◽  
B. B. Diena ◽  
A. C. Maniar ◽  
L. Greenberg
Keyword(s):  
Thin Layer ◽  
Quantitative Determination ◽  
Test Organism ◽  
Microbiological Assay ◽  
Nutrient Agar ◽  
Glycerol Solution ◽  
Zone Size ◽  
Chemical Methods ◽  
Chemical Assay

A microbiological assay has been developed using the techniques of assay for antibiotics. Metal cylinders were placed on plates prepared by overlaying a thin layer of seed agar, inoculated with a test organism, on a base of nutrient agar. Appropriate dilutions of a standard glycerol solution and of the glycerol preparations being tested were added to the proper cylinders. The plates were incubated and the zone sizes around the cylinders measured. The potency of the preparations was determined by comparing the average zone size of the unknown preparations with that of the standard. The method proved to be simple to perform, and in the absence of interfering substances, yielded essentially the same results as chemical methods. A number of carbohydrates—glucose, laevulose, maltose, mannitol, mannose, saccharose, and trehalose—interfered with assay results so that the method cannot be used for glycerol determinations in mixtures where they are present. No interference, however, was noted with d-arabitol and erythritol, which were found to interfere with the chemical assay.

scholarly journals Microbiological Assay for Lomefloxacin in Coated Tablets

2006 ◽  
Vol 89 (4) ◽  
pp. 1077-1079 ◽  
Author(s):  
Greici Cristiani Gomes ◽  
Hérida Regina Nunes Salgado
Keyword(s):  
Bacillus Subtilis ◽  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Method Validation ◽  
Test Organism ◽  
Microbiological Assay ◽  
Plate Method ◽  
Relative Standard ◽  
Bacillus Subtilis Atcc

Abstract The validation of a microbiological assay, applying cylinder plate method for determination of the activity of lomefloxacin in coated tablets is described. Using a strain of Bacillus subtilis ATCC 9372 as the test organism, lomefloxacin was measured in concentrations ranging from 2.0 to 8.0 μg/mL. The method validation showed that it is linear (r = 0.9999), precise (relative standard deviation = 1.15%), and accurate (it measured the added quantities). The excipients did not interfere in the determination. It was concluded that the microbiological assay is satisfactory for quantitation of lomefloxacin in tablets.

Comparison of Methods for Determination of Lysine in Cereals

1963 ◽  
Vol 46 (3) ◽  
pp. 399-405
Author(s):  
Y Pomeranz ◽  
B S Miller
Keyword(s):  
Ion Exchange ◽  
Paper Chromatography ◽  
Microbiological Assay ◽  
Two Dimensional ◽  
Comparison Of Methods ◽  
Microbiological Method ◽  
Exchange Method ◽  
Wheat Products ◽  
Ion Exchange Method

Abstract The lysine contents of 10 milled wheat products and cereal foods were determined by microbiological, enzymatic, two-dimensional paper chromatographic, and ion exchange chromatographic methods. The assay of lysine by two-dimensional paper chromatography produced very low results. The results from the microbiological method were comparable to those by the ion exchange method. The decarboxylase method consistently gave low results which averaged 82% of those obtained by the microbiological assay. Possible reasons for these low results are presented and discussed.

scholarly journals Microbiological Assay for Cefoxitin Sodium in Dosage Form

2007 ◽  
Vol 90 (2) ◽  
pp. 452-455 ◽  
Author(s):  
Hrida Regina Nunes Salgado ◽  
Greici Cristiani Gomes Tozo
Keyword(s):  
Staphylococcus Epidermidis ◽  
Dosage Form ◽  
Test Organism ◽  
Microbiological Assay ◽  
Plate Method

Abstract A microbiological assay applying the cylinder-plate method is described for determination of the activity of cefoxitin sodium in injectables. Using a strain of Staphylococcus epidermidis ATCC 12226 as the test organism, cefoxitin sodium was measured in concentrations ranging from 50.0 to 200.0 g/mL. The validation showed that the method was linear (r = 0.9998), precise (RSD = 0.81%), and accurate. It was concluded that the microbiological assay is satisfactory for quantitation of cefoxitin sodium in injectables.

scholarly journals Determination of biotin content in beet molasses by Lactobacillus plantarum

2005 ◽  
pp. 215-220
Author(s):  
Eva Loncar ◽  
Irena Dosenovic ◽  
Sinisa Markov ◽  
Radomir Malbasa ◽  
Ljiljana Kolarov
Keyword(s):  
Lactobacillus Plantarum ◽  
Liquid Culture ◽  
Liquid Media ◽  
Microbiological Method ◽  
Beet Molasses ◽  
Standard Solutions

D-biotin content in beet molasses was determined by microbiological method using Lactobacillus plantarum, based on the comparison of the growth of this microorganism in molasses solutions with those in standard solutions of biotin. Incubation of the microorganism was performed on original Vitamin Biotin Testbouillon and laboratory prepared liquid culture medias. The amount of "real" biotin in molasses is low. The results depend upon the sample and volume of molasses solutions. Biotin contents obtained on both liquid media are close.

Comparison of Chemical and Microbiological Methods for the Determination of Procaine Penicillin in Premixes and Mixed Feeds

1963 ◽  
Vol 46 (3) ◽  
pp. 429-433
Author(s):  
Stanley E Katz
Keyword(s):  
Chemical Method ◽  
Microbiological Assay ◽  
Assay Method ◽  
Microbiological Method ◽  
Procaine Penicillin ◽  
Assay Procedure ◽  
Microbiological Methods ◽  
Mixed Feeds

Abstract A chemical and a microbiological method of analysis for procaine penicillin in premixes and mixed feeds have been compared. The microbiological assay method was a typical cylinderplate assay procedure. The chemical method was based upon the conversion by base of penicillins to penicilloic acids. In the eight premixes studied, the chemical-to-microbiological ratio of results varied from 0.92 to 1.17. In four mixed feeds, the ratio varied from 0.97 to 1.09. In general, the chemical method yielded slightly higher results than the microbiological method. There was little difference between methods in regard to reproducibility and accuracy.

Microbiological Method for Assaying Nystatin in Animal Feeds

1963 ◽  
Vol 46 (3) ◽  
pp. 438-444
Author(s):  
Joseph F Pagano
Keyword(s):  
Microbiological Assay ◽  
Microbiological Method ◽  
Animal Feeds ◽  
Assay Procedure ◽  
Feed Samples

Abstract A microbiological assay procedure for the determination of nystatin2 in animal feeds at levels as low as 2 5 ppm has been developed. The assay procedure and two feed samples were submitted to five collaborating laboratories for evaluation. The results of the collaborative assay study are presented, and it is recommended that the method be adopted as official, first action.

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