Adhesion, proliferation, and osteogenic differentiation of human mesenchymal stem cells on additively manufactured Ti6Al4V alloy scaffolds modified with calcium phosphate nanoparticles

Dexamethasone (DEX)-loaded biphasic calcium phosphate nanoparticles (BCP-NPs) are prepared by incorporation of DEX during or after the formation of BCP-NPs. The DEX-loaded BCP-NPs release DEX in a sustained manner and enhance the osteogenic differentiation of hMSCs.

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Gene transfection of human mesenchymal stem cells with a nano-hydroxyapatite-collagen scaffold containing DNA-functionalized calcium phosphate nanoparticles

Genes to Cells ◽

10.1111/gtc.12374 ◽

2016 ◽

Vol 21 (7) ◽

pp. 682-695 ◽

Cited By ~ 23

Author(s):

Taichi Tenkumo ◽

Juan Ramón Vanegas Sáenz ◽

Yukyo Takada ◽

Masatoshi Takahashi ◽

Olga Rotan ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Calcium Phosphate ◽

Gene Transfection ◽

Human Mesenchymal Stem Cells ◽

Collagen Scaffold ◽

Calcium Phosphate Nanoparticles

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Two-layer membranes of calcium phosphate/collagen/PLGA nanofibres: in vitro biomineralisation and osteogenic differentiation of human mesenchymal stem cells

Nanoscale ◽

10.1039/c0nr00615g ◽

2011 ◽

Vol 3 (2) ◽

pp. 401-409 ◽

Cited By ~ 53

Author(s):

Nora Hild ◽

Oliver D. Schneider ◽

Dirk Mohn ◽

Norman A. Luechinger ◽

Fabian M. Koehler ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Calcium Phosphate ◽

Osteogenic Differentiation ◽

Human Mesenchymal Stem Cells

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Effect of Platelet-rich Plasma on the in vitro Proliferation and Osteogenic Differentiation of Human Mesenchymal Stem Cells on Distinct Calcium Phosphate Scaffolds: The Specific Surface Area Makes a Difference

Journal of Biomaterials Applications ◽

10.1177/0885328207088269 ◽

2008 ◽

Vol 23 (2) ◽

pp. 169-188 ◽

Cited By ~ 50

Author(s):

Philip Kasten ◽

Julia Vogel ◽

Ingo Beyen ◽

Stefan Weiss ◽

Philipp Niemeyer ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Calcium Phosphate ◽

Specific Surface ◽

Osteogenic Differentiation ◽

Surface Area ◽

Platelet Rich Plasma ◽

Human Mesenchymal Stem Cells ◽

In Vitro Proliferation

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Silicate-substituted calcium phosphate with enhanced strut porosity stimulates osteogenic differentiation of human mesenchymal stem cells

Journal of Materials Science Materials in Medicine ◽

10.1007/s10856-015-5387-5 ◽

2015 ◽

Vol 26 (1) ◽

Cited By ~ 16

Author(s):

Roberta Ferro De Godoy ◽

Stacy Hutchens ◽

Charlie Campion ◽

Gordon Blunn

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Calcium Phosphate ◽

Osteogenic Differentiation ◽

Human Mesenchymal Stem Cells

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Synergistic acceleration in the osteogenesis of human mesenchymal stem cells by graphene oxide–calcium phosphate nanocomposites

Chemical Communications ◽

10.1039/c4cc02442g ◽

2014 ◽

Vol 50 (62) ◽

pp. 8484-8487 ◽

Cited By ~ 61

Author(s):

Rameshwar Tatavarty ◽

Hao Ding ◽

Guijin Lu ◽

Robert J. Taylor ◽

Xiaohong Bi

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Graphene Oxide ◽

Stem Cell ◽

Cell Differentiation ◽

Calcium Phosphate ◽

Synergistic Effect ◽

Human Mesenchymal Stem Cells ◽

Stem Cell Differentiation ◽

Calcium Phosphate Nanoparticles

Nanocomposites consisting of oblong ultrathin plate shaped calcium phosphate nanoparticles and graphene oxide microflakes were synthesized and have demonstrated markedly synergistic effect in accelerating stem cell differentiation to osteoblasts.

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Osteogenic potential of poly(ethylene glycol)-amorphous calcium phosphate composites on human mesenchymal stem cells

Journal of Tissue Engineering ◽

10.1177/2041731420926840 ◽

2020 ◽

Vol 11 ◽

pp. 204173142092684 ◽

Cited By ~ 3

Author(s):

Aman S Chahal ◽

Manuel Schweikle ◽

Aina-Mari Lian ◽

Janne E Reseland ◽

Håvard J Haugen ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Calcium Phosphate ◽

Ethylene Glycol ◽

Osteogenic Differentiation ◽

Amorphous Calcium Phosphate ◽

Human Mesenchymal Stem Cells ◽

Osteogenic Potential ◽

Poly Ethylene Glycol ◽

Poly Ethylene

Synthetic hydrogel-amorphous calcium phosphate composites are promising candidates to substitute biologically sourced scaffolds for bone repair. While the hydrogel matrix serves as a template for stem cell colonisation, amorphous calcium phosphate s provide mechanical integrity with the potential to stimulate osteogenic differentiation. Here, we utilise composites of poly(ethylene glycol)-based hydrogels and differently stabilised amorphous calcium phosphate to investigate potential effects on attachment and osteogenic differentiation of human mesenchymal stem cells. We found that functionalisation with integrin binding motifs in the form of RGD tripeptide was necessary to allow adhesion of large numbers of cells in spread morphology. Slow dissolution of amorphous calcium phosphate mineral in the scaffolds over at least 21 days was observed, resulting in the release of calcium and zinc ions into the cell culture medium. While we qualitatively observed an increasingly mineralised extracellular matrix along with calcium deposition in the presence of amorphous calcium phosphate-loaded scaffolds, we did not observe significant changes in the expression of selected osteogenic markers.

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