Carmine spider mite Tetranychus cinnabarinus control: Laboratory and field efficacy and biochemical characterization of 2, 4-di-tertbutylphenol and ethyl oleate

2021 ◽  
Vol 139 ◽  
pp. 105390
Author(s):  
Yijuan Chen ◽  
Jiaxuan Luo ◽  
Nianfeng Wan ◽  
Jiexian Jiang ◽  
Guanghui Dai
Keyword(s):  
Spider Mite ◽  
Biochemical Characterization ◽  
Ethyl Oleate ◽  
Tetranychus Cinnabarinus ◽  
Control Laboratory ◽  
Field Efficacy ◽  
Carmine Spider Mite

Characterization of an Intradiol Ring-Cleavage Dioxygenase Gene Associated With Abamectin Resistance in Tetranychus cinnabarinus (Acari: Tetranychidae)

2019 ◽  
Vol 112 (4) ◽  
pp. 1858-1865
Author(s):  
Zhifeng Xu ◽  
Peilin Liu ◽  
Yuan Hu ◽  
Jia Hu ◽  
Cuicui Qi ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Spider Mite ◽  
Gene Sequence ◽  
Ring Cleavage ◽  
Tetranychus Cinnabarinus ◽  
Limited Data ◽  
Dioxygenase Gene ◽  
Carmine Spider Mite ◽  
Rnai Technique

AbstractTetranychus cinnabarinus (Boisduval), i.e., carmine spider mite, is a worldwide pest that can cause serious damage to plants. Problems of resistance have arisen since abamectin usage in the control of T. cinnabarinus. Unfortunately, there are only limited data on the extent of this problem. To understand the development of abamectin resistance in the carmine spider mite, we prokaryotically expressed an intradiol ring-cleavage dioxygenase (ID-RCD) gene sequence, TcID-RCD1, which had a significant upregulated expression of over 7.7 times in an abamectin-resistant strain (AbR) when compared with that of a susceptible strain (SS). The crude enzyme activity also indicated that the AbR had a higher activity than that exhibited in SS. When susceptible individuals were treated with abamectin, TcID-RCD1 was also overexpressed. Furthermore, using the RNA interference (RNAi) technique, TcID-RCD1 was successfully knocked down, with the expression level decreasing significantly to approximately 39% in the SS strain compared with the control. And the mortality of mites feeding on dsTcID-RCD1 increased significantly when treated with abamectin. These results strongly suggest that TcID-RCD1 is involved in abamectin resistance in T. cinnabarinus.

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