89. Application of cryopreservation techniques for long-term storage of clinical isolates of Candida fungi

A study was designed to determine if current dormant-bud cryopreservation techniques investigated on woody plants, such as apple (Malus domestica), gooseberry (Ribes), blueberry (Vaccinium corymbosum) and pear (Pryus communis) etc., could be applied to certain nut tree species for long-term preservation. Pecan (Carya illinoinensis) and black walnut (Juglans nigra) were exposed to prefreezing temperatures ranging from -10° C to -40° C and then directly immersed in liquid nitrogen for 2 hrs. Dehydration by prefreezing was not sufficient for bud survival in pecan. Bud survival was increased by dehydrating stem sections prior to prefreezing. Prefreezing at -30° or -40° C was suitable for survival of black walnut.

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Novel Approaches to the Cryopreservation of Human Spermatozoa: History and Development of the Spermatozoa Vitrification Technology

Journal of Reproductive and Stem Cell Biotechnology ◽

10.1177/205891581100200207 ◽

2011 ◽

Vol 2 (2) ◽

pp. 128-145 ◽

Cited By ~ 5

Author(s):

Evgenia Isachenko ◽

Gohar Rahimi ◽

Peter Mallmann ◽

Raul Sanchez ◽

Vladimir Isachenko

Keyword(s):

Ice Crystals ◽

Intracellular Lipids ◽

Cryoprotective Agents ◽

Long Term Storage ◽

Cryopreservation Techniques ◽

Water Quality And Quantity ◽

And Storage ◽

Term Storage ◽

Conventional Freezing

Cryobiology is very intensively applied in reproductive and veterinary medicine for preservation of gametes, embryos and reproductive tissues. Sub-zero temperatures combined with appropriate cryoprotective agents preserve the physiological and reproductive functions of the cells making long-term storage possible without loss of viability. With the use of cryoprotective agents it has become possible to develop cryopreservation techniques, such as the slow conventional freezing and vitrification that are in use in the present times. In slow controlled-rate conventional freezing extracellular ice crystals are formed whereas in vitrification no ice crystals are formed. Glass formation is compatible with the survival of the cell and the preservation of its intracellular structures provided the type(s) and concentrations of cryoprotectant used are not chemo- or osmotoxic. However, irrespective of the type of cooling method employed the cryosurvival of cells and tissues is influenced by the size and maturity of cells, amounts of intracellular water, quality and quantity of intracellular lipids, type of cells, their function and morphology. The intracellular milieu of cryopreserved cells and tissues remain less understood. The application of nanotechnology may help reveal and help advance our knowledge of the cryobiological principles involved in cryosurvival. At this moment the methods of cryopreservation that merit further investigation are vitrification and lyophilization. Vitrification is cheap if reagents are prepared in-house and the procedure can be performed rapidly. It has been successfully applied for gametes and embryos (of different stages of development), and reproductive cells/tissues, somatic cells and stem cells. However, vitrification is more demanding technically and requires operation and storage at sub-zero temperatures. On the other hand lyophilization deserves further investigation because it is a cheaper form of cryopreservation that may enable cryostorage at less demanding temperatures of 4°C and may even allow transport at ambient temperature. These possibilities are explored in this review.

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Pemanfaatan Kriopreservasi untuk Penyimpanan Bibit Anggrek Jangka Panjang

JURNAL BIOS LOGOS ◽

10.35799/jbl.11.2.2020.28793 ◽

2020 ◽

Vol 10 (2) ◽

pp. 71

Author(s):

Arkan Setiaji ◽

Rr Rifka Annisa

Keyword(s):

Review Article ◽

Breeding Programs ◽

Cold Temperatures ◽

Orchid Species ◽

Long Term Storage ◽

Cryopreservation Techniques ◽

Cryopreservation Protocol ◽

Term Storage

ABSTRAKTanaman anggrek (Orchidaceae) telah menjadi komoditas penting di pasar perdagangan internasional karena keeksotisan dan daya tahan bunganya. Meskipun keragaman anggrek spesies menduduki peringkat kedua setelah Asteraceae dan hibridanya menduduki peringkat pertama, namun anggrek menghadapi ancaman kepunahan dan penurunan kualitas genetik. Program pemuliaan, seperti persilangan dan penyisipan gen unggul, serta perbanyakan in vitro perlu didukung dengan cara memastikan ketersediaan eksplan. Dalam rangka penyimpanan untuk kebutuhan jangka panjang, eksplan dapat disimpan menggunakan teknik kriopreservasi. Review ini bertujuan untuk memberikan gambaran umum terhadap kemajuan penelitian di bidang kriopreservasi, khususnya tanaman anggrek. Melalui program penyimpanan jangka panjang, gen-gen dan eksplan unggulan baik untuk pengembangan anggrek hibrida atau khususnya untuk tujuan konservasi anggrek langka bisa dipastikan ketersediaannya. Dalam artikel review ini telah dibahas berbagai riset tentang teknik kriopreservasi pada biji, protokorm, dan serbuk sari anggrek. Kemajuan pengembangan teknik telah dicapai, namun masih diperlukan banyak modifikasi agar suatu protokol kriopreservasi dapat diterapkan pada semua spesies dan hibrida anggrek.Kata kunci: kriopreservasi; anggrek; suhu dingin; eksplan ABSTRACTOrchid plants (Orchidaceae) have become important commodities in the international trades market because of their exoticism and flower endurance. Although the diversity of orchid species is ranked second after Asteraceae and number of hybrids are ranked first, but orchids face the threat of extinction and genetic degradation. Breeding programs, including such as crossing and insertion of superior genes, and in vitro propagation need to be supported by ensuring the availability of explants. In the context of maintaining storage for long-term needs, explants can be stored using cryopreservation techniques. This review aims to provide an overview of the progress of research in the field of cryopreservation, specifically orchid plants. Through a long-term storage program, superior genes and explants both for the development of hybrid orchids or in particular for the purpose of conservation of rare orchids can be ensured availability. In this review article various researches on cryopreservation techniques in seeds, protocorms, and pollen have been discussed in this article. Advances in technical development have been achieved, but many modifications are still needed so that a cryopreservation protocol can be applied to all orchid species and hybrids.Keywords: cryopreservation; orchids; cold temperatures; explants

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Modelling of Moisture Movement in Wood during Outdoor Storage

Nonlinear Analysis Modelling and Control ◽

10.15388/na.2001.6.1.15210 ◽

2001 ◽

Vol 6 (2) ◽

pp. 3-14 ◽

Cited By ~ 23

Author(s):

R. Baronas ◽

F. Ivanauskas ◽

I. Juodeikienė ◽

A. Kajalavičius

Keyword(s):

Experimental Data ◽

Finite Difference ◽

Climatic Conditions ◽

Two Dimensional ◽

Moisture Movement ◽

Finite Difference Technique ◽

Long Term Storage ◽

Space Formulation ◽

Term Storage

A model of moisture movement in wood is presented in this paper in a two-dimensional-in-space formulation. The finite-difference technique has been used in order to obtain the solution of the problem. The model was applied to predict the moisture content in sawn boards from pine during long term storage under outdoor climatic conditions. The satisfactory agreement between the numerical solution and experimental data was obtained.

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