Preplant Fertilization Increases Substrate Microbial Respiration But Does Not Affect Southern Highbush Blueberry Establishment in a Coconut Coir-based Substrate

Coconut coir is widely used as a substrate component for southern highbush blueberry [(SHB) Vaccinium corymbosum L. interspecific hybrids] cultivation in containers. Coconut coir-based substrates can exhibit high potassium (K), sodium (Na), and chlorine (Cl) concentrations. Sodium in the substrate is particularly problematic because it can cause salinity stress and nutritional imbalances in young blueberry plants. Thus, Na removal is important to ensure transplant success. We hypothesized that preplant fertilization with large volumes of nutrient solution can reduce substrate salinity, replace Na with nutritional cations, and enhance blueberry establishment. We tested this hypothesis in a greenhouse experiment with ‘Snowchaser’ SHB grown in rhizoboxes filled with a 7:3 mix of coconut coir and perlite. Four different treatments were delivered every 24 hours starting 72 hours before transplant. Treatments included 1.75 g⋅L–1 calcium nitrate (CN), 2.38 g⋅L–1 monoammonium phosphate (MAP), deionized water, and well water. One rooted cutting was transplanted to each rhizobox. Rhizoboxes were fertigated during the 7-week cultivation period. We found that preplant fertilization increased nitrogen (N), phosphorus (P), and calcium (Ca) concentrations in the substrate without replacing Na. Thus, preplant fertilization increased substrate salinity. Preplant fertilization also promoted microbial respiration in the substrate at the start of the experiment. Treatments did not affect SHB root architecture, leaf area index, leaf greenness, or biomass accumulation, likely because nutrients delivered by the fertigation solution provided the plants with homogeneous optimal conditions. These findings suggest that preplant fertilization with large volumes of nutrient solution does not enhance blueberry establishment in coconut coir-based substrates.

PRELIMINARY RESULTS REGARDING THE FRUIT QUALITY AND YIELD OF SOME BLUEBERRY GENOTYPES

Vaccinium corymbosum is one of the berry species with economic and food interest to Romanian growers and consumers. The development of blueberrty crop is a result of breeding activity, which has generated many cultivars of quality fruit and high productivity. The characters that are of economic interest in the cultivation of blueberries depend on the area of cultivation where the new cultivars are obtained. The study was conducted in summer 2020 in an experimental plot established in 2015 with seven blueberry genotypes, at Research Institute for Fruit Growing, Pitesti, Romania in the Small Fruit Laboratory, having an experience in the field of more than 40 years. The objective at this study is to compare some of fruit characteristics from genotypes 'Azur', 'Delicia', 'Duke', 'Northblue', 'Simultan', '4/6' and '6/38': plant yield, average fruit weight, fruit length, fruit diameter, the soluble solids, fruit acidity or pH, fruit firmness. During this study 'Delicia' cv. proved to have the highest production per plant (4.20 kg plant), the highest values of fruit diameter (19.70 mm), and the highest value of fruit total titratable acidity (01.25 %).

Fruit Quality and Yield of Three Highbush Blueberry (Vaccinium corymbosum L.) Cultivars Grown in Two Planting Systems under Different Protected Environments

Due to the increasing interest in highbush blueberry (Vaccinium corymbosum L.) among consumers, together with the problems of climate change and specific substrate requirements, a novel approach to intensive blueberry production is required. Here, ‘Duke’, ‘Aurora’, and ‘Brigitta’ blueberry cultivars were planted under the protective environments of a high tunnel and black hail net, each using ridge and pot planting systems. The high tunnel increased the maximal air temperature on average by 7.2 °C compared to the hail net. For all three cultivars, harvest began 6 to 18 days earlier under the high tunnel than under the hail net; however, lower yields and individual phenolics contents were obtained for the fruit. In ‘Aurora’ and ‘Brigitta’, environmental conditions under the high tunnel also reduced plant volume and fruit sugar/organic acid ratio. Growing blueberry plants in 60 L pots had no negative effects on plant volume and fruit ripening time, yield, firmness, color, and chemical composition. This study represents the first to compare highbush blueberry grown under the high tunnel and hail net protective environments using ridge and pot planting systems across three different cultivars. Here, we can conclude that optimal highbush blueberry production of ‘Duke’, ‘Aurora’, and ‘Brigitta’ under the climate conditions of the study provides earlier ripening times under the high tunnel. However, according to fruit yield and quality, all three cultivars benefit from the hail net over the high tunnel, while ‘Duke’ and ‘Brigitta’ also benefit in particular from the hail net combined with growth in pots.

Chromosome Doubling in Genetically Diverse Bilberry (Vaccinium myrtillus L.) Accessions and Evaluation of Tetraploids in Terms of Phenotype and Ability to Cross with Highbush Blueberry (V. corymbosum L.)

To expand the gene pool and introduce new traits to the tetraploid cultivars of Vaccinium corymbosum from wild diploid species V. myrtillus, it is necessary to double the chromosome number in diploid species in order to overcome a post zygotic crossing barrier and a strong triploid block, existing within the genus Vaccinium. Five genetically diverse bilberry genotypes were selected from 21 accessions taken from the breeding collection of the National Institute of Horticultural Research (Skierniewice, Poland) for this study. The bilberry genotypes were derived from the Polish locations of Bolimów Landscape Park, Budy Grabskie and forest complex Zwierzyniec (Łódź Province), and habitats in Norway. The selection of genotypes was made based on the analysis of amplified fragment length polymorphism (AFLP-PCR). Analysis of the Jaccard similarity indexes and the UPGMA method revealed that the examined accessions formed two main groups on the dendrogram. The first group consisted of accessions from Norway, while the second group agglomerated Polish accessions. A further two classes were distinguished in the Polish group: the first included accessions from Budy Grabskie and the second from Zwierzyniec, located ca. 9 km from Budy Grabskie. In order to obtain plant material for in vitro polyploidisation, in vitro shoot cultures of the selected accessions were initiated and multiplied. Both antimitotics used, colchicine and APM, induced tetraploids for all of the accessions. The obtained tetraploids were multiplied, rooted ex vitro and grown in a greenhouse and then in a field. The first flowering was observed in 1.5-year-old plants, either diploid or tetraploid. Diploids bloomed slightly earlier and more profusely than tetraploid plants. Compared to diploids, autotetraploids had significantly larger flowers by ca. 64% and larger pollen tetrads by ca. 35%. The germination capacity of pollen tetrads was high in tetraploids (87.8%), although slightly lower than in diploids (94.3%). After pollinating the flowers of three highbush blueberry cultivars with pollen from the bilberry tetraploid accession, J-4-4x, the plants formed fruits, some of which contained properly formed seeds. The effectiveness of interspecific crossing between V. corymbosum and tetraploid V. myrtillus, defined as the percentage of obtained seedlings in relation to the number of pollinated flowers, was highest (53.3%) in the blueberry ‘Liberty’, and lower in ‘Bluecrop’ and ‘Northland’, 14.8% and 10.0%, respectively. Before using the seedlings for further breeding, their hybridity will be confirmed by molecular markers and the phenotype will be evaluated.

Multicomponent Polyphenolic Extracts from Vaccinium corymbosum at Lab and Pilot Scale. Characterization and Effectivity against Nosocomial Pathogens

An extraction method was designed and scaled up to produce multicomponent polyphenolic extracts from blueberries (Vaccinium corymbosum) of three different varieties. The process was specifically drawn up to comply with green chemistry principles. Extracts were obtained for the direct assessment of their antimicrobial and antiadhesive activities, and their direct use in the control of infections caused by concerning multidrug-resistant nosocomial pathogens. Analytical characterization was performed by liquid chromatography–tandem mass spectrometry (LC–MS/MS). Similar qualitative profiles were obtained in the three studied varieties with some significant quantitative differences. Up to 22 different polyphenols were identified with a clear predominance of anthocyani(di)ns followed by flavanols, non-flavonoids, and far behind by flavan-3-ols and procyanidins. The individual content of the main polyphenols was also discussed. A pilot scale extract has been also produced as a proof-of-concept, showing that scaling-up triples the content of bioactive phytochemicals. The effect of the polyphenolic extracts was analyzed against seven multidrug-resistance bacterial species by performing biofilm formation and growth and killing curves assays. All the studied varieties showed antibacterial and antiadhesive activities, being the extract containing the highest concentration of bioactive polyphenols, the most active with a high bactericidal effect.