scholarly journals Essential Function of Mec1, the Budding Yeast ATM/ATR Checkpoint-Response Kinase, in Protein Homeostasis

2018 ◽  
Vol 46 (4) ◽  
pp. 495-503.e2 ◽  
Author(s):  
Isaac Corcoles-Saez ◽  
Kangzhen Dong ◽  
Anthony L. Johnson ◽  
Erik Waskiewicz ◽  
Michael Costanzo ◽  
...  
Keyword(s):  
Budding Yeast ◽  
Protein Homeostasis ◽  
Checkpoint Response ◽  
Essential Function

scholarly journals Phosphorylation of the Budding Yeast 9-1-1 Complex Is Required for Dpb11 Function in the Full Activation of the UV-Induced DNA Damage Checkpoint

2008 ◽  
Vol 28 (15) ◽  
pp. 4782-4793 ◽  
Author(s):  
Fabio Puddu ◽  
Magda Granata ◽  
Lisa Di Nola ◽  
Alessia Balestrini ◽  
Gabriele Piergiovanni ◽  
...  
Keyword(s):  
Dna Damage ◽  
Budding Yeast ◽  
Dna Damage Checkpoint ◽  
Checkpoint Activation ◽  
Checkpoint Response ◽  
Checkpoint Proteins ◽  
M Phase ◽  
Mutant Cells ◽  
Full Activation

ABSTRACT Following genotoxic insults, eukaryotic cells trigger a signal transduction cascade known as the DNA damage checkpoint response, which involves the loading onto DNA of an apical kinase and several downstream factors. Chromatin modifications play an important role in recruiting checkpoint proteins. In budding yeast, methylated H3-K79 is bound by the checkpoint factor Rad9. Loss of Dot1 prevents H3-K79 methylation, leading to a checkpoint defect in the G1 phase of the cell cycle and to a reduction of checkpoint activation in mitosis, suggesting that another pathway contributes to Rad9 recruitment in M phase. We found that the replication factor Dpb11 is the keystone of this second pathway. dot1Δ dpb11-1 mutant cells are sensitive to UV or Zeocin treatment and cannot activate Rad53 if irradiated in M phase. Our data suggest that Dpb11 is held in proximity to damaged DNA through an interaction with the phosphorylated 9-1-1 complex, leading to Mec1-dependent phosphorylation of Rad9. Dpb11 is also phosphorylated after DNA damage, and this modification is lost in a nonphosphorylatable ddc1-T602A mutant. Finally, we show that, in vivo, Dpb11 cooperates with Dot1 in promoting Rad9 phosphorylation but also contributes to the full activation of Mec1 kinase.

scholarly journals Bypassing anaphase by fission yeast cut9 mutation: requirement of cut9+ to initiate anaphase.

1994 ◽  
Vol 127 (6) ◽  
pp. 1655-1670 ◽  
Author(s):  
I Samejima ◽  
M Yanagida
Keyword(s):  
Amino Acid ◽  
Fission Yeast ◽  
Budding Yeast ◽  
Temperature Sensitive ◽  
Cytoplasmic Microtubule ◽  
Tetratricopeptide Repeats ◽  
Late Anaphase ◽  
Amino Acid Repeats ◽  
Cold Sensitive ◽  
Essential Function

A novel anaphase block phenotype was found in fission yeast temperature-sensitive cut9 mutants. Cells enter mitosis with chromosome condensation and short spindle formation, then block anaphase, but continue to progress into postanaphase events such as degradation of the spindle, reformation of the postanaphase cytoplasmic microtubule arrays, septation, and cytokinesis. The cut9 mutants are defective in the onset of anaphase and possibly in the restraint of postanaphase events until the completion of anaphase. The cut9+ gene encodes a 78-kD protein containing the 10 34-amino acid repeats, tetratricopeptide repeats (TPR), and similar to budding yeast Cdc16. It is essential for viability, and the mutation sites reside in the TPR. The three genes, namely, nuc2+, scn1+, and scn2+, genetically interact with cut9+. The nuc2+ and cut9+ genes share an essential function to initiate anaphase. The cold-sensitive scn1 and scn2 mutations, defective in late anaphase, can suppress the ts phenotype of cut9.

scholarly journals Correction to: Budding yeast Rad51: a paradigm for how phosphorylation and intrinsic structural disorder regulate homologous recombination and protein homeostasis

2021 ◽  
Author(s):  
Tai-Ting Woo ◽  
Chi-Ning Chuang ◽  
Ting-Fang Wang
Keyword(s):  
Homologous Recombination ◽  
Budding Yeast ◽  
Structural Disorder ◽  
Protein Homeostasis

The article: Budding yeast Rad51: a paradigm for how phosphorylation and intrinsic structural disorder regulate homologous recombination and protein homeostasis.

scholarly journals Septins, under Cla4p Regulation, and the Chitin Ring Are Required for Neck Integrity in Budding Yeast

2003 ◽  
Vol 14 (5) ◽  
pp. 2128-2141 ◽  
Author(s):  
Martin Schmidt ◽  
Archana Varma ◽  
Tomás Drgon ◽  
Blair Bowers ◽  
Enrico Cabib
Keyword(s):  
Cell Cycle ◽  
Cell Death ◽  
Protein Kinase ◽  
Synthetic Lethality ◽  
Budding Yeast ◽  
Chitin Synthase ◽  
Vital Function ◽  
Septin Ring ◽  
Bud Neck ◽  
Essential Function

CLA4, encoding a protein kinase of the PAK type, and CDC11, encoding a septin, were isolated in a screen for synthetic lethality with CHS3, which encodes the chitin synthase III catalytic moiety. Although Ste20p shares some essential function with Cla4p, it did not show synthetic lethality with Chs3p. cla4 and cdc11 mutants exhibited similar morphological and septin localization defects, including aberrant and ectopic septa. Myo1p, which requires septins for localization, formed abnormally wide rings in cla4 mutants. In cultures started with unbudded cells, an inhibitor of Chs3p activity, nikkomycin Z, aggravated the abnormalities of cla4 and cdc11 mutants and gave rise to enlarged necks at the mother-bud junction, leading to cell death. It is concluded that Cla4p is required for the correct localization and/or assembly of the septin ring and that both the septin ring and the Chs3p-requiring chitin ring at the mother-bud neck cooperate in maintaining the neck constricted throughout the cell cycle, a vital function in budding yeast.

scholarly journals Vtc5 Is Localized to the Vacuole Membrane by the Conserved AP-3 Complex to Regulate Polyphosphate Synthesis in Budding Yeast

mBio ◽  
2021 ◽  
Author(s):  
Amanda Bentley-DeSousa ◽  
Michael Downey
Keyword(s):  
Energy Metabolism ◽  
Cell Signaling ◽  
Budding Yeast ◽  
Protein Homeostasis ◽  
Vacuole Membrane ◽  
Inorganic Phosphates

Long polymers of inorganic phosphates called polyphosphates are ubiquitous across biological kingdoms. From bacteria to humans, they have diverse functions related to protein homeostasis, energy metabolism, and cell signaling.

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