scholarly journals RSK-MASTL Pathway Delays Meiotic Exit in Mouse Zygotes to Ensure Paternal Chromosome Stability

2018 ◽  
Vol 47 (3) ◽  
pp. 363-376.e5 ◽  
Author(s):  
Shou Soeda ◽  
Kaori Yamada-Nomoto ◽  
Tatsuo Michiue ◽  
Miho Ohsugi
Keyword(s):  
Chromosome Stability ◽  
Paternal Chromosome ◽  
Mouse Zygotes

Chromosome Stability in Some Hexaploid Strains of Triticale 1

Crop Science ◽  
1969 ◽  
Vol 9 (2) ◽  
pp. 235-236 ◽  
Author(s):  
T. Tsuchiya ◽  
E. N. Larter
Keyword(s):  
Chromosome Stability

Chromosome Stability and Fertility of a Nitrous Oxide‐Derived Tetraploid Population of Red Clover 1

Crop Science ◽  
1983 ◽  
Vol 23 (1) ◽  
pp. 45-48 ◽  
Author(s):  
N. Giri ◽  
N. L. Taylor ◽  
G. B. Collins
Keyword(s):  
Nitrous Oxide ◽  
Red Clover ◽  
Chromosome Stability ◽  
Tetraploid Population

scholarly journals Totipotency of mouse zygotes extends to single blastomeres of embryos at the four-cell stage

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marino Maemura ◽  
Hiroaki Taketsuru ◽  
Yuki Nakajima ◽  
Ruiqi Shao ◽  
Ayaka Kakihara ◽  
...  
Keyword(s):  
Inner Cell Mass ◽  
Cell Mass ◽  
Mouse Embryos ◽  
Primitive Endoderm ◽  
Cell Stage ◽  
Supportive Environment ◽  
Mouse Zygotes ◽  
Single Blastomeres ◽  
Multicellular Organisms

AbstractIn multicellular organisms, oocytes and sperm undergo fusion during fertilization and the resulting zygote gives rise to a new individual. The ability of zygotes to produce a fully formed individual from a single cell when placed in a supportive environment is known as totipotency. Given that totipotent cells are the source of all multicellular organisms, a better understanding of totipotency may have a wide-ranging impact on biology. The precise delineation of totipotent cells in mammals has remained elusive, however, although zygotes and single blastomeres of embryos at the two-cell stage have been thought to be the only totipotent cells in mice. We now show that a single blastomere of two- or four-cell mouse embryos can give rise to a fertile adult when placed in a uterus, even though blastomere isolation disturbs the transcriptome of derived embryos. Single blastomeres isolated from embryos at the eight-cell or morula stages and cultured in vitro manifested pronounced defects in the formation of epiblast and primitive endoderm by the inner cell mass and in the development of blastocysts, respectively. Our results thus indicate that totipotency of mouse zygotes extends to single blastomeres of embryos at the four-cell stage.

scholarly journals STAT3 Is an Upstream Regulator of Granzyme G in the Maternal-To-Zygotic Transition of Mouse Embryos

2021 ◽  
Vol 22 (1) ◽  
pp. 460
Author(s):  
Huan Ou-Yang ◽  
Shinn-Chih Wu ◽  
Li-Ying Sung ◽  
Shiao-Hsuan Yang ◽  
Shang-Hsun Yang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Fluorescent Protein ◽  
Mouse Embryos ◽  
Cell Nuclei ◽  
Cell Stage ◽  
Promoter Assay ◽  
Maternal To Zygotic Transition ◽  
Mouse Zygotes ◽  
Time Specific

The maternal-to-zygotic transition (MZT), which controls maternal signaling to synthesize zygotic gene products, promotes the preimplantation development of mouse zygotes to the two-cell stage. Our previous study reported that mouse granzyme g (Gzmg), a serine-type protease, is required for the MZT. In this study, we further identified the maternal factors that regulate the Gzmg promoter activity in the zygote to the two-cell stage of mouse embryos. A full-length Gzmg promoter from mouse genomic DNA, FL-pGzmg (−1696~+28 nt), was cloned, and four deletion constructs of this Gzmg promoter, Δ1-pGzmg (−1369~+28 nt), Δ2-pGzmg (−939~+28 nt), Δ3-pGzmg (−711~+28 nt) and Δ4-pGzmg (−417~+28 nt), were subsequently generated. Different-sized Gzmg promoters were used to perform promoter assays of mouse zygotes and two-cell stage embryos. The results showed that Δ4-pGzmg promoted the highest expression level of the enhanced green fluorescent protein (EGFP) reporter in the zygotes and two-cell embryos. The data suggested that time-specific transcription factors upregulated Gzmg by binding cis-elements in the −417~+28-nt Gzmg promoter region. According to the results of the promoter assay, the transcription factor binding sites were predicted and analyzed with the JASPAR database, and two transcription factors, signal transducer and activator of transcription 3 (STAT3) and GA-binding protein alpha (GABPα), were identified. Furthermore, STAT3 and GABPα are expressed and located in zygote pronuclei and two-cell nuclei were confirmed by immunofluorescence staining; however, only STAT3 was recruited to the mouse zygote pronuclei and two-cell nuclei injected with the Δ4-pGzmg reporter construct. These data indicated that STAT3 is a maternal transcription factor and may upregulate Gzmg to promote the MZT. Furthermore, treatment with a STAT3 inhibitor, S3I-201, caused mouse embryonic arrest at the zygote and two-cell stages. These results suggest that STAT3, a maternal protein, is a critical transcription factor and regulates Gzmg transcription activity in preimplantation mouse embryos. It plays an important role in the maternal-to-zygotic transition during early embryonic development.

Propanediol alters intracellular pH and developmental potential of mouse zygotes independently of volume change

1990 ◽  
Vol 5 (2) ◽  
pp. 212-216 ◽  
Author(s):  
M. Damien ◽  
A.A. Luciano ◽  
J.J. Peluso
Keyword(s):  
Intracellular Ph ◽  
Volume Change ◽  
Developmental Potential ◽  
Mouse Zygotes

scholarly journals rarmutations which increase artificial chromosome stability inSaccharomyces cerevisiaeidentify transcription and recombination proteins

1991 ◽  
Vol 19 (7) ◽  
pp. 1385-1391 ◽  
Author(s):  
David Kipling ◽  
Cathryn Tambini ◽  
Stephen E. Kearsey
Keyword(s):  
Artificial Chromosome ◽  
Chromosome Stability ◽  
Recombination Proteins

scholarly journals Control of chromosome stability by the β-TrCP–REST–Mad2 axis

Nature ◽  
2008 ◽  
Vol 452 (7185) ◽  
pp. 365-369 ◽  
Author(s):  
Daniele Guardavaccaro ◽  
David Frescas ◽  
N. Valerio Dorrello ◽  
Angelo Peschiaroli ◽  
Asha S. Multani ◽  
...  
Keyword(s):  
Chromosome Stability
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