First isolation and characterization of vancomycin-resistant Enterococcus faecium harboring vanD5 gene cluster recovered from a 79-year-old female inpatient in Japan

2019 ◽  
Vol 95 (4) ◽  
pp. 114883
Author(s):  
Satoko Terada ◽  
Tetsuya Harada ◽  
Masaharu Yokota ◽  
Takako Tsuchiya ◽  
Kaori Adachi ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Enterococcus Faecium ◽  
Vancomycin Resistant Enterococcus ◽  
Isolation And Characterization ◽  
Vancomycin Resistant

scholarly journals Molecular Characterization of Vancomycin-Resistant Enterococcus faecium Isolates from Korea

2005 ◽  
Vol 43 (5) ◽  
pp. 2303-2306 ◽  
Author(s):  
K. S. Ko ◽  
J. Y. Baek ◽  
J.-Y. Lee ◽  
W. S. Oh ◽  
K. R. Peck ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Enterococcus Faecium ◽  
Vancomycin Resistant Enterococcus ◽  
Vancomycin Resistant

scholarly journals Molecular characterization of vancomycin-resistant Enterococcus faecium isolates from Bermuda

PLoS ONE ◽  
2017 ◽  
Vol 12 (3) ◽  
pp. e0171317 ◽  
Author(s):  
Patrick Eberechi Akpaka ◽  
Shivnarine Kissoon ◽  
Clyde Wilson ◽  
Padman Jayaratne ◽  
Ashley Smith ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Enterococcus Faecium ◽  
Vancomycin Resistant Enterococcus ◽  
Vancomycin Resistant

Characterization of the First VanB Vancomycin-Resistant Enterococcus faecium Isolated in a Spanish Hospital

2004 ◽  
Vol 48 (3) ◽  
pp. 199-203 ◽  
Author(s):  
Fabi�n Lorenzo-D�az ◽  
Teresa Delgado ◽  
Jos� A. Reyes-Darias ◽  
Carlos Flores ◽  
Sebasti�n M�ndez-Alvarez ◽  
...  
Keyword(s):  
Enterococcus Faecium ◽  
Vancomycin Resistant Enterococcus ◽  
Spanish Hospital ◽  
Vancomycin Resistant

scholarly journals Molecular Characterization of Vancomycin Resistant Enterococcus faecium Isolates from....

2018 ◽  
Author(s):  
Mehtap Ünlü Söğüt ◽  
Şule Kırca ◽  
Selma Keleş Uludağ ◽  
Gökcen Dinç ◽  
Alper Çiftçi
Keyword(s):  
Molecular Characterization ◽  
Enterococcus Faecium ◽  
Vancomycin Resistant Enterococcus ◽  
Vancomycin Resistant

Vancomycin-resistant Enterococcus faecium colonizing patients on hospital admission in Germany: prevalence and molecular epidemiology

2020 ◽  
Vol 75 (10) ◽  
pp. 2743-2751 ◽  
Author(s):  
Kyriaki Xanthopoulou ◽  
Silke Peter ◽  
David Tobys ◽  
Michael Behnke ◽  
Ariane G Dinkelacker ◽  
...  
Keyword(s):  
Hospital Admission ◽  
Molecular Epidemiology ◽  
Enterococcus Faecium ◽  
Vancomycin Resistant Enterococcus ◽  
University Hospitals ◽  
Carriage Rate ◽  
Federal States ◽  
Vancomycin Resistant ◽  
Rectal Colonization

Abstract Objectives To analyse the rectal carriage rate and the molecular epidemiology of vancomycin-resistant Enterococcus faecium (VREfm) recovered from patients upon hospital admission. Methods Adult patients were screened at six German university hospitals from five different federal states upon hospital admission for rectal colonization with VREfm between 2014 and 2018. Molecular characterization of VREfm was performed by WGS followed by MLST and core-genome MLST analysis. Results Of 16350 patients recruited, 263 were colonized with VREfm, with increasing prevalence rates during the 5 year study period (from 0.8% to 2.6%). In total, 78.5% of the VREfm were vanB positive and 20.2% vanA positive, while 1.2% harboured both vanA and vanB. The predominant ST was ST117 (56.7%) followed by ST80 (15%), ST203 (10.9%), ST78 (5.7%) and ST17 (3.2%). ST117/vanB VREfm isolates formed a large cluster of 96 closely related isolates extending across all six study centres and four smaller clusters comprising 13, 5, 4 and 3 isolates each. In contrast, among the other STs inter-regional clonal relatedness was rarely observed. Conclusions To our knowledge, this is the largest admission prevalence and molecular epidemiology study of VREfm. These data provide insight into the epidemiology of VREfm at six German university hospitals and demonstrate the remarkable inter-regional clonal expansion of the ST117/vanB VREfm clone.

Identification and characterization of IS1476, an insertion sequence-like element that disrupts VanY function in a vancomycin-resistant Enterococcus faecium strain.

1997 ◽  
Vol 41 (8) ◽  
pp. 1805-1807 ◽  
Author(s):  
M G MacKinnon ◽  
M A Drebot ◽  
G J Tyrrell
Keyword(s):  
Resistance Gene ◽  
Enterococcus Faecium ◽  
Insertion Sequence ◽  
Vancomycin Resistant Enterococcus ◽  
Detection Strategy ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant ◽  
Identification And Characterization ◽  
Faecium Strain

The vanY gene of vancomycin-resistant enterococci encodes a D,D-carboxypeptidase. By using a PCR detection strategy, a VanA Enterococcus faecium clinical isolate was found to have an insertion sequence (IS)-like element designated IS1476 in vanY. The activity of the VanY D,D-carboxypeptidase in this isolate was decreased in a fluorometric fluoraldehyde o-phthalaldehyde assay with diacetyl-L-Lys-D-Ala-D-Ala as the substrate. This, to our knowledge, is the first report of an IS-like element in a vancomycin resistance gene.

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