Data on optimized production and characterization of alkaline proteases from newly isolated alkaliphiles from Lonar soda lake, India

ABSTRACT In the Southeast of Mexico, there are many native cichlids with commercial interest such as redhead cichlid (Vieja melanurus) and twoband cichlid (V. bifasciata), which have a great local demand and excellent meat quality. However, it is necessary to implement their culture based on nutrition studies and digestive biochemistry. This study’s objective was to characterize these two cichlids’ digestive proteases (pH, temperature, and inhibitors) through biochemistry techniques. Results showed that V. melanurus and V. bifasciata have a digestive capacity analogous to other omnivore fishes, where the optimal pH values of stomach proteases (4 and 2, respectively) and intestinal proteases (6 and 12, respectively), the optimal temperature of acid (35°C and 55°C, respectively) and alkaline proteases (45°C and 55°C, respectively) are quite similar. Both species presented high thermal and pH stabilities. Inhibition showed that V. melanurus is more sensitive to specific inhibitors for alkaline proteases than V. bifasciata. In conclusion, V. bisfasciata and V. melanurus have different digestive protease patterns. Both species can hydrolyze different protein ingredients to formulate a specific diet. Nevertheless, V. bifasciata is more resistant to the presence of inhibitors, which allow it to include vegetable proteins in its diet.

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Purification and characterization of two extracellular alkaline proteases from a newly isolated obligate alkalophilic Bacillus sphaericus

Journal of Industrial Microbiology & Biotechnology ◽

10.1038/sj.jim.7000151 ◽

2001 ◽

Vol 26 (6) ◽

pp. 387-393 ◽

Cited By ~ 17

Author(s):

J Singh ◽

R M Vohra ◽

D K Sahoo

Keyword(s):

Bacillus Sphaericus ◽

Alkaline Proteases ◽

Purification And Characterization ◽

Alkalophilic Bacillus

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Production and Partial Characterization of an Alkaline Xylanase from a Novel FungusCladosporium oxysporum

BioMed Research International ◽

10.1155/2016/4575024 ◽

2016 ◽

Vol 2016 ◽

pp. 1-7 ◽

Cited By ~ 12

Author(s):

Guo-Qiang Guan ◽

Peng-Xiang Zhao ◽

Jin Zhao ◽

Mei-Juan Wang ◽

Shu-Hao Huo ◽

...

Keyword(s):

Nitrogen Source ◽

Wheat Bran ◽

Internal Transcribed Spacer ◽

Gene Sequence ◽

Agricultural Waste ◽

Xylanase Activity ◽

Maximum Activity ◽

Alkaline Proteases ◽

Xylanase Production

A new fungusCladosporium oxysporumGQ-3 producing extracellular xylanase was isolated from decaying agricultural waste and identified based on the morphology and comparison of internal transcribed spacer (ITS) rDNA gene sequence.C. oxysporumproduced maximum xylanase activity of 55.92 U/mL with wheat bran as a substrate and NH4Cl as a nitrogen source. Mg2+improvedC. oxysporumxylanase production.Partially purified xylanase exhibited maximum activity at 50°C and pH 8.0, respectively, and showed the stable activity after 2-h treatment in pH 7.0–8.5 or below 55°C. Mg2+enhanced the xylanase activity by 2% while Cu2+had the highest inhibition ratio of 57.9%. Furthermore,C. oxysporumxylanase was resistant to most of tested neutral and alkaline proteases. Our findings indicated thatCladosporium oxysporumGQ-3 was a novel xylanase producer, which could be used in the textile processes or paper/feed industries.

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