PSCDGs of mouse multipotent adult germline stem cells can enter and progress through meiosis to form haploid male germ cells in vitro

2010 ◽  
Vol 80 (4-5) ◽  
pp. 184-194 ◽  
Author(s):  
Jessica Nolte ◽  
Hans W. Michelmann ◽  
Marieke Wolf ◽  
Gerald Wulf ◽  
Karim Nayernia ◽  
...  
Keyword(s):  
Stem Cells ◽  
Germ Cells ◽  
Germline Stem Cells ◽  
Male Germ Cells ◽  
Haploid Male

scholarly journals Spermatogonial Stem Cells for In Vitro Spermatogenesis and In Vivo Restoration of Fertility

Cells ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 745 ◽  
Author(s):  
Fahar Ibtisham ◽  
Ali Honaramooz
Keyword(s):  
Stem Cells ◽  
Germ Cells ◽  
Recent Progress ◽  
Male Germ Cells ◽  
Culture Systems ◽  
In Vitro Spermatogenesis ◽  
Haploid Male ◽  
Restoration Of Fertility

Spermatogonial stem cells (SSCs) are the only adult stem cells capable of passing genes onto the next generation. SSCs also have the potential to provide important knowledge about stem cells in general and to offer critical in vitro and in vivo applications in assisted reproductive technologies. After century-long research, proof-of-principle culture systems have been introduced to support the in vitro differentiation of SSCs from rodent models into haploid male germ cells. Despite recent progress in organotypic testicular tissue culture and two-dimensional or three-dimensional cell culture systems, to achieve complete in vitro spermatogenesis (IVS) using non-rodent species remains challenging. Successful in vitro production of human haploid male germ cells will foster hopes of preserving the fertility potential of prepubertal cancer patients who frequently face infertility due to the gonadotoxic side-effects of cancer treatment. Moreover, the development of optimal systems for IVS would allow designing experiments that are otherwise difficult or impossible to be performed directly in vivo, such as genetic manipulation of germ cells or correction of genetic disorders. This review outlines the recent progress in the use of SSCs for IVS and potential in vivo applications for the restoration of fertility.

In vitro differentiation process of human Wharton’s jelly mesenchymal stem cells to male germ cells in the presence of gonadal and non-gonadal conditioned media with retinoic acid

2015 ◽  
Vol 51 (10) ◽  
pp. 1093-1101 ◽  
Author(s):  
Fardin Amidi ◽  
Nahid Ataie Nejad ◽  
Marziyeh Agha Hoseini ◽  
Karim Nayernia ◽  
Zohreh Mazaheri ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Retinoic Acid ◽  
Germ Cells ◽  
Conditioned Media ◽  
Differentiation Process ◽  
In Vitro Differentiation ◽  
Male Germ Cells ◽  
Wharton's Jelly

scholarly journals Derivation of male germ cells from induced pluripotent stem cells in vitro and in reconstituted seminiferous tubules

2012 ◽  
Vol 45 (2) ◽  
pp. 91-100 ◽  
Author(s):  
S. Yang ◽  
J. Bo ◽  
H. Hu ◽  
X. Guo ◽  
R. Tian ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Germ Cells ◽  
Pluripotent Stem Cells ◽  
Seminiferous Tubules ◽  
Male Germ Cells ◽  
Induced Pluripotent

scholarly journals Generation of male germ cells from induced pluripotent stem cells (iPS cells): an in vitro and in vivo study

2012 ◽  
Vol 14 (4) ◽  
pp. 574-579 ◽  
Author(s):  
Yong Zhu ◽  
Hong-Liang Hu ◽  
Peng Li ◽  
Shi Yang ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Germ Cells ◽  
Pluripotent Stem Cells ◽  
Ips Cells ◽  
In Vivo Study ◽  
Male Germ Cells ◽  
Induced Pluripotent

Retinoic acid and/or progesterone differentiate mouse induced pluripotent stem cells into male germ cells in vitro

2019 ◽  
Vol 121 (3) ◽  
pp. 2159-2169
Author(s):  
Javad Amini Mahabadi ◽  
Abolfazl Aazami Tameh ◽  
Sayyed Alireza Talaei ◽  
Mohammad Karimian ◽  
Tahereh Rahiminia ◽  
...  
Keyword(s):  
Stem Cells ◽  
Retinoic Acid ◽  
Induced Pluripotent Stem Cells ◽  
Germ Cells ◽  
Pluripotent Stem Cells ◽  
Male Germ Cells ◽  
Induced Pluripotent

In vitro and in vivo differentiation of induced pluripotent stem cells into male germ cells

2013 ◽  
Vol 433 (3) ◽  
pp. 286-291 ◽  
Author(s):  
Heng Cai ◽  
Xiaoyu Xia ◽  
Li Wang ◽  
Yue Liu ◽  
Zuping He ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Germ Cells ◽  
Pluripotent Stem Cells ◽  
Male Germ Cells ◽  
In Vivo Differentiation ◽  
Induced Pluripotent

Lentiviral vector transfection of chicken embryo primordial germ cells in vitro

2008 ◽  
Vol 5 (1) ◽  
pp. 1-5
Author(s):  
Ding Hong-Mei ◽  
Xu Shi-Yong ◽  
Shao Gen-Bao ◽  
Sun Yan ◽  
Wang Meng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Transfer ◽  
Germ Cells ◽  
Lentiviral Vector ◽  
Transfection Efficiency ◽  
Primordial Germ Cells ◽  
Embryonic Stem ◽  
Germline Stem Cells ◽  
Transfer Vectors

AbstractLentiviruses as gene transfer vectors have been used successfully to transfect mammal embryonic stem cells and germline stem cells, but this has not been attempted in avian primordial germ cells (PGCs). PGCs were isolated from the gonads of Isa-brown chicken embryos at stage 28 and co-cultured with gonadal stroma cells. A lentiviral vector pLenti-CMV-EGFP was constructed and the virus harvested by cotransfecting 293FT cells with the vector and packaging plasmids. Concentrated lentiviruses were used to transfect chicken PGCs, the transfection efficiency was up to 24.19%.

scholarly journals Offspring production of haploid spermatid-like cells derived from mouse female germline stem cells with chromatin condensation

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Xiaopeng Hu ◽  
Hu Wang ◽  
Geng. G. Tian ◽  
Changliang Hou ◽  
Bo Xu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Retinoic Acid ◽  
Y Chromosome ◽  
Germ Cells ◽  
Chromatin Condensation ◽  
Germline Stem Cells ◽  
Chromosome Conformation ◽  
Female Germline

Abstract Background During male meiosis, the Y chromosome can form perfect pairing with the X chromosome. However, it is unclear whether mammalian Female germline stem cells (FGSCs) without a Y chromosome can transdifferentiate into functional haploid spermatid-like cells (SLCs). Results We found that spermatogenesis was restarted by transplanting FGSCs into Kitw/wv mutant testes. Complete meiosis and formation of SLCs was induced in vitro by testicular cells of Kitw/wv mutant mice, cytokines and retinoic acid. Healthy offspring were produced by sperm and SLCs derived from the in vivo and in vitro transdifferentiation of FGSCs, respectively. Furthermore, high-throughput chromosome conformation capture sequencing(Hi-C-seq) and “bivalent” (H3K4me3-H3K27me3) micro chromatin immunoprecipitation sequencing (μChIP-seq) experiments showed that stimulated by retinoic acid gene 8 (STRA8)/protamine 1 (PRM1)-positive transdifferentiated germ cells (tGCs) and male germ cells (mGCs) display similar chromatin dynamics and chromatin condensation during in vitro spermatogenesis. Conclusion This study demonstrates that sperm can be produced from FGSCs without a Y chromosome. This suggests a strategy for dairy cattle breeding to produce only female offspring with a high-quality genetic background.

Overexpression of STRA8, BOULE, and DAZL Genes Promotes Goat Bone Marrow–Derived Mesenchymal Stem Cells In Vitro Transdifferentiation Toward Putative Male Germ Cells

2016 ◽  
Vol 24 (2) ◽  
pp. 300-312 ◽  
Author(s):  
Pei-zhen Li ◽  
Guang-yao Yan ◽  
Le Han ◽  
Jing Pang ◽  
Bu-shuai Zhong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Germ Cells ◽  
Male Germ Cells
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