P133. The role of Nogo on the guidance of commissural axons in the mouse spinal cord

2010 ◽  
Vol 80 ◽  
pp. S62
Author(s):  
L.Q. Wang ◽  
S.O. Chan
Keyword(s):  
Spinal Cord ◽  
Mouse Spinal Cord ◽  
Commissural Axons

scholarly journals Evidence for a Role of srGAP3 in the Positioning of Commissural Axons within the Ventrolateral Funiculus of the Mouse Spinal Cord

PLoS ONE ◽  
2011 ◽  
Vol 6 (5) ◽  
pp. e19887 ◽  
Author(s):  
Claire Bacon ◽  
Volker Endris ◽  
Irwin Andermatt ◽  
Vera Niederkofler ◽  
Robert Waltereit ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Mouse Spinal Cord ◽  
Commissural Axons ◽  
Ventrolateral Funiculus

Tetanus toxin binding to mouse spinal cord cells: an evaluation of the role of gangliosides in toxin internalization

1988 ◽  
Vol 475 (1) ◽  
pp. 118-127 ◽  
Author(s):  
R.G. Parton ◽  
C.D. Ockleford ◽  
D.R. Critchley
Keyword(s):  
Spinal Cord ◽  
Tetanus Toxin ◽  
Mouse Spinal Cord ◽  
Toxin Binding

Ventral midline cells are required for the local control of commissural axon guidance in the mouse spinal cord

Development ◽  
1999 ◽  
Vol 126 (16) ◽  
pp. 3649-3659
Author(s):  
M.P. Matise ◽  
M. Lustig ◽  
T. Sakurai ◽  
M. Grumet ◽  
A.L. Joyner
Keyword(s):  
Spinal Cord ◽  
Critical Role ◽  
Floor Plate ◽  
Posterior Commissure ◽  
Ventral Midline ◽  
Mouse Spinal Cord ◽  
Commissural Axons ◽  
Midline Cells

Specialized cells at the midline of the central nervous system have been implicated in controlling axon projections in both invertebrates and vertebrates. To address the requirement for ventral midline cells in providing cues to commissural axons in mice, we have analyzed Gli2 mouse mutants, which lack specifically the floor plate and immediately adjacent interneurons. We show that a Dbx1 enhancer drives tau-lacZ expression in a subpopulation of commissural axons and, using a reporter line generated from this construct, as well as DiI tracing, we find that commissural axons projected to the ventral midline in Gli2(−/−) embryos. Netrin1 mRNA expression was detected in Gli2(−/−) embryos and, although much weaker than in wild-type embryos, was found in a dorsally decreasing gradient. This result demonstrates that while the floor plate can serve as a source of long-range cues for C-axons in vitro, it is not required in vivo for the guidance of commissural axons to the ventral midline in the mouse spinal cord. After reaching the ventral midline, most commissural axons remained clustered in Gli2(−/−) embryos, although some were able to extend longitudinally. Interestingly, some of the longitudinally projecting axons in Gli2(−/−) embryos extended caudally and others rostrally at the ventral midline, in contrast to normal embryos in which virtually all commissural axons turn rostrally after crossing the midline. This finding indicates a critical role for ventral midline cells in regulating the rostral polarity choice made by commissural axons after they cross the midline. In addition, we provide evidence that interactions between commissural axons and floor plate cells are required to modulate the localization of Nr-CAM and TAG-1 proteins on axons at the midline. Finally, we show that the floor plate is not required for the early trajectory of motoneurons or axons of the posterior commissure, whose projections are directed away from the ventral midline in both WT and Gli2(−/−) embryos, although they are less well organized in Gli2(−/−)mutants.

Guidance of commissural growth cones at the floor plate in embryonic rat spinal cord

Development ◽  
1990 ◽  
Vol 109 (2) ◽  
pp. 435-447 ◽  
Author(s):  
P. Bovolenta ◽  
J. Dodd
Keyword(s):  
Spinal Cord ◽  
Axon Guidance ◽  
Plate Boundary ◽  
Growth Cones ◽  
Floor Plate ◽  
Morphological Properties ◽  
Rat Spinal Cord ◽  
Commissural Axons ◽  
Carbocyanine Dye

The floor plate of the embryonic rat spinal cord has been proposed to act as an intermediate target that plays a role in the pattern of extension of commissural axons. To begin to examine the role of the floor plate in axon guidance at the midline, we have studied the precision of the commissural axon projection to and across the floor plate during development. To delineate the pathway, the fluorescent carbocyanine dye, Di-I, has been used as a probe. We show that commissural axons traverse the floor plate and turn rostrally at its contralateral border with remarkable precision. Axons were not observed to turn ipsilaterally and turned only upon reaching the contralateral edge of the floor plate. Virtually all commissural axons follow this route. The morphology of commissural growth cones was also examined. As they encountered the floor plate, commissural growth cones became larger and increased in complexity. The reorientation of axons in register with the floor plate boundary and the change in the morphological properties of commissural growth cones as they traverse the midline suggest that the floor plate may act as a guidepost with functions similar to cells that have been implicated in axon guidance in invertebrates.

Nogo-B is the major form of Nogo at the floor plate and likely mediates crossing of commissural axons in the mouse spinal cord

2017 ◽  
Vol 525 (13) ◽  
pp. 2915-2928 ◽  
Author(s):  
Liqing Wang ◽  
Chao Yu ◽  
Jun Wang ◽  
Peggy Leung ◽  
Ding Ma ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Floor Plate ◽  
Mouse Spinal Cord ◽  
Commissural Axons ◽  
Major Form

Role of the phosphatidylinositol-specific phospholipase C pathway in δ-opioid receptor-mediated antinociception in the mouse spinal cord

Neuroscience ◽  
2000 ◽  
Vol 99 (2) ◽  
pp. 327-331 ◽  
Author(s):  
M Narita ◽  
M Ohsawa ◽  
H Mizoguchi ◽  
T Aoki ◽  
T Suzuki ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Phospholipase C ◽  
Opioid Receptor ◽  
Mouse Spinal Cord ◽  
Δ Opioid Receptor

scholarly journals The role of B-class Eph receptors and ligands on midline guidance in the embryonic mouse spinal cord

2006 ◽  
Vol 295 (1) ◽  
pp. 365
Author(s):  
Stephanie Kadison ◽  
Rudiger Klein ◽  
Mark Henkemeyer ◽  
Zaven Kaprielian
Keyword(s):  
Spinal Cord ◽  
Eph Receptors ◽  
Embryonic Mouse ◽  
Mouse Spinal Cord

Distinct physiological role of amidino-TAPA-sensitive and DAMGO-insensitive μ-opioid receptor splice variants in the mouse spinal cord

2013 ◽  
Vol 711 (1-3) ◽  
pp. 80-86 ◽  
Author(s):  
Hirokazu Mizoguchi ◽  
Chizuko Watanabe ◽  
Takayuki Higashiya ◽  
Satoshi Takeda ◽  
Kaori Moriyama ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Opioid Receptor ◽  
Splice Variants ◽  
Physiological Role ◽  
Mouse Spinal Cord ◽  
Μ Opioid Receptor

scholarly journals The role of floor plate contact in the elaboration of contralateral commissural projections within the embryonic mouse spinal cord

2006 ◽  
Vol 296 (2) ◽  
pp. 499-513 ◽  
Author(s):  
Stephanie R. Kadison ◽  
Fujio Murakami ◽  
Michael P. Matise ◽  
Zaven Kaprielian
Keyword(s):  
Spinal Cord ◽  
Floor Plate ◽  
Embryonic Mouse ◽  
Mouse Spinal Cord
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