Design strategy of multifunctional and high efficient hydrogen sulfide NIR fluorescent probe and its application in vivo

2021 ◽  
Vol 185 ◽  
pp. 108901
Author(s):  
Keyan Zhou ◽  
Yutao Yang ◽  
Tingting Zhou ◽  
Ming Jin ◽  
Caixia Yin
Author(s):  
Han Zhang ◽  
Mei Zhu ◽  
Dao-Yong Jiang ◽  
Xing-Ying Xue ◽  
Jiao Zhang ◽  
...  

The Analyst ◽  
2015 ◽  
Vol 140 (21) ◽  
pp. 7165-7169 ◽  
Author(s):  
Tianbao Liu ◽  
Jie Lin ◽  
Zhen Li ◽  
Lin Lin ◽  
Yuning Shen ◽  
...  

We have developed a novel colorimetric and ratiometric fluorescence probe for the selective and sensitive imaging of H2S in living cells and living zebrafish in vivo.


The Analyst ◽  
2020 ◽  
Vol 145 (15) ◽  
pp. 5123-5127 ◽  
Author(s):  
Yuming Zhang ◽  
Yuncong Chen ◽  
Hongbao Fang ◽  
Xiangchao Shi ◽  
Hao Yuan ◽  
...  

CouDE could monitor the endogenous H2S level change induced by alteration of CBS enzyme activity in living cells and in vivo.


2017 ◽  
Vol 23 (41) ◽  
pp. 9872-9878 ◽  
Author(s):  
Shahi Imam Reja ◽  
Neetu Sharma ◽  
Muskan Gupta ◽  
Payal Bajaj ◽  
Vandana Bhalla ◽  
...  

2018 ◽  
Vol 277 ◽  
pp. 647-653 ◽  
Author(s):  
Xixi Xie ◽  
Caixia Yin ◽  
Yongkang Yue ◽  
Jianbin Chao ◽  
Fangjun Huo

2017 ◽  
Vol 89 ◽  
pp. 919-926 ◽  
Author(s):  
Chul Soon Park ◽  
Tai Hwan Ha ◽  
Seon-Ae Choi ◽  
Duong Nguyen Nguyen ◽  
Seonmyeong Noh ◽  
...  

2020 ◽  
Vol 63 (5) ◽  
pp. 741-750 ◽  
Author(s):  
Luyan Wu ◽  
Wenhui Zeng ◽  
Liandong Feng ◽  
Yuxuan Hu ◽  
Yidan Sun ◽  
...  

2015 ◽  
Vol 68 ◽  
pp. 681-687 ◽  
Author(s):  
Palanisamy Sathyadevi ◽  
Yu-Jen Chen ◽  
Shou-Cheng Wu ◽  
Yen-Hao Chen ◽  
Yun-Ming Wang

2019 ◽  
Vol 133 (20) ◽  
pp. 2045-2059 ◽  
Author(s):  
Da Zhang ◽  
Xiuli Wang ◽  
Siyao Chen ◽  
Selena Chen ◽  
Wen Yu ◽  
...  

Abstract Background: Pulmonary artery endothelial cell (PAEC) inflammation is a critical event in the development of pulmonary arterial hypertension (PAH). However, the pathogenesis of PAEC inflammation remains unclear. Methods: Purified recombinant human inhibitor of κB kinase subunit β (IKKβ) protein, human PAECs and monocrotaline-induced pulmonary hypertensive rats were employed in the study. Site-directed mutagenesis, gene knockdown or overexpression were conducted to manipulate the expression or activity of a target protein. Results: We showed that hydrogen sulfide (H2S) inhibited IKKβ activation in the cell model of human PAEC inflammation induced by monocrotaline pyrrole-stimulation or knockdown of cystathionine γ-lyase (CSE), an H2S generating enzyme. Mechanistically, H2S was proved to inhibit IKKβ activity directly via sulfhydrating IKKβ at cysteinyl residue 179 (C179) in purified recombinant IKKβ protein in vitro, whereas thiol reductant dithiothreitol (DTT) reversed H2S-induced IKKβ inactivation. Furthermore, to demonstrate the significance of IKKβ sulfhydration by H2S in the development of PAEC inflammation, we mutated C179 to serine (C179S) in IKKβ. In purified IKKβ protein, C179S mutation of IKKβ abolished H2S-induced IKKβ sulfhydration and the subsequent IKKβ inactivation. In human PAECs, C179S mutation of IKKβ blocked H2S-inhibited IKKβ activation and PAEC inflammatory response. In pulmonary hypertensive rats, C179S mutation of IKKβ abolished the inhibitory effect of H2S on IKKβ activation and pulmonary vascular inflammation and remodeling. Conclusion: Collectively, our in vivo and in vitro findings demonstrated, for the first time, that endogenous H2S directly inactivated IKKβ via sulfhydrating IKKβ at Cys179 to inhibit nuclear factor-κB (NF-κB) pathway activation and thereby control PAEC inflammation in PAH.


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