A new sensor based on phenolic hydroxy and imine groups was used as an efficient colorimetric and “turn on” fluorescent sensor, which exhibited high selectivity and sensitivity for F−.
By utilizing laboratory-guided evolution, we have converted the fluorescent proton-pumping rhodopsin GR from Gloeobacter violaceus into GR1, a red-shifted, turn-on fluorescent sensor for chloride.
A new calixpyridinium-based light-responsive host–guest recognition motif was found in this work. This host–guest recognition motif was further discovered to be applied as a selective turn-on fluorescent sensor for lysine.
A single point mutation converts a proton-pumping rhodopsin into a red-shifted, turn-on fluorescent sensor for chloride