Assessment of cyto- and genotoxic effects of Cesium-133 in Vicia faba using single-cell gel electrophoresis and random amplified polymorphic DNA assays

2020 ◽  
Vol 197 ◽  
pp. 110620 ◽  
Author(s):  
Yu Zhang ◽  
Jin-long Lai ◽  
Xiao-hui Ji ◽  
Xue-gang Luo
Keyword(s):  
Single Cell ◽  
Vicia Faba ◽  
Gel Electrophoresis ◽  
Random Amplified Polymorphic Dna ◽  
Genotoxic Effects ◽  
Single Cell Gel Electrophoresis ◽  
Dna Assays

Metabolic activation of herbicide products by Vicia faba detected in human peripheral lymphocytes using alkaline single cell gel electrophoresis

2007 ◽  
Vol 21 (6) ◽  
pp. 1143-1154 ◽  
Author(s):  
María Elena Calderón-Segura ◽  
Sandra Gómez-Arroyo ◽  
Bertha Molina-Alvarez ◽  
Rafael Villalobos-Pietrini ◽  
Carmen Calderón-Ezquerro ◽  
...  
Keyword(s):  
Single Cell ◽  
Vicia Faba ◽  
Gel Electrophoresis ◽  
Metabolic Activation ◽  
Peripheral Lymphocytes ◽  
Single Cell Gel Electrophoresis ◽  
Human Peripheral Lymphocytes

Effect of Allyl Isothiocyanate (AITC) in Both Nitrite- and Nitrosamine-Induced Cell Death, Production of Reactive Oxygen Species, and DNA Damage by the Single-Cell Gel Electrophoresis (SCGE): Does It Have Any Protective Effect on HepG2 Cells?

2010 ◽  
Vol 29 (3) ◽  
pp. 305-312
Author(s):  
Pınar Erkekoğlu ◽  
Terken Baydar
Keyword(s):  
Reactive Oxygen Species ◽  
Protective Effect ◽  
Single Cell ◽  
Gel Electrophoresis ◽  
Hepg2 Cells ◽  
Reactive Oxygen ◽  
Allyl Isothiocyanate ◽  
Oxygen Species ◽  
Genotoxic Effects ◽  
Single Cell Gel Electrophoresis

The current study was designed to investigate possible protective effect of allyl isothiocyanate (AITC) in nitrite- and nitrosamine-treated human hepatoma cells (HepG2) with the evaluation by cytotoxic effects and genotoxic effects determined by the single-cell gel electrophoresis (SCGE). Allyl isothiocyanate treatment enhanced cell viability and reduced intracellular reactive oxygen species (ROS) production in both nitrite- and nitrosamine-treated cells significantly. In SCGE, when compared to untreated control cells, all of the treated groups caused increases in the tail intensity (%) such as nitrite at 17%, N-nitrosodimethylamine (NDMA) at 279%, N-nitrosodiethylamine (NDEA) at 324%, and N-nitrosomorpholine (NMOR) at 288%. Allyl isothiocyanate reduced the tail intensity caused by nitrite 36%, by NDMA 36%, by NDEA 49%, and by NMOR 32%, respectively, when compared to each individual toxic compound–treated group. In conclusion, AITC protected HepG2 cells against cytotoxic and genotoxic effects caused by nitrite and the nitrosamines.

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