An Investigation of the Genotoxic Effects of Airborne Particulate Matter Using Single-Cell Gel Electrophoresis

2001 ◽  
Vol 10 (3-4) ◽  
pp. 258-260
Author(s):  
Alison Curnow ◽  
Barbara Parsons ◽  
Leo Salter ◽  
Nick Morley ◽  
David Gould
Keyword(s):  
Particulate Matter ◽  
Single Cell ◽  
Gel Electrophoresis ◽  
Airborne Particulate Matter ◽  
Airborne Particulate ◽  
Genotoxic Effects ◽  
Single Cell Gel Electrophoresis

Effect of Allyl Isothiocyanate (AITC) in Both Nitrite- and Nitrosamine-Induced Cell Death, Production of Reactive Oxygen Species, and DNA Damage by the Single-Cell Gel Electrophoresis (SCGE): Does It Have Any Protective Effect on HepG2 Cells?

2010 ◽  
Vol 29 (3) ◽  
pp. 305-312
Author(s):  
Pınar Erkekoğlu ◽  
Terken Baydar
Keyword(s):  
Reactive Oxygen Species ◽  
Protective Effect ◽  
Single Cell ◽  
Gel Electrophoresis ◽  
Hepg2 Cells ◽  
Reactive Oxygen ◽  
Allyl Isothiocyanate ◽  
Oxygen Species ◽  
Genotoxic Effects ◽  
Single Cell Gel Electrophoresis

The current study was designed to investigate possible protective effect of allyl isothiocyanate (AITC) in nitrite- and nitrosamine-treated human hepatoma cells (HepG2) with the evaluation by cytotoxic effects and genotoxic effects determined by the single-cell gel electrophoresis (SCGE). Allyl isothiocyanate treatment enhanced cell viability and reduced intracellular reactive oxygen species (ROS) production in both nitrite- and nitrosamine-treated cells significantly. In SCGE, when compared to untreated control cells, all of the treated groups caused increases in the tail intensity (%) such as nitrite at 17%, N-nitrosodimethylamine (NDMA) at 279%, N-nitrosodiethylamine (NDEA) at 324%, and N-nitrosomorpholine (NMOR) at 288%. Allyl isothiocyanate reduced the tail intensity caused by nitrite 36%, by NDMA 36%, by NDEA 49%, and by NMOR 32%, respectively, when compared to each individual toxic compound–treated group. In conclusion, AITC protected HepG2 cells against cytotoxic and genotoxic effects caused by nitrite and the nitrosamines.

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