scholarly journals Isolation and characterization of Vibrio alginolyticus strain HAT3 causing skin ulceration disease in cultured sea cucumber Holothuria atra (Jaeger, 1833)

2021 ◽  
Author(s):  
Nayer M. Fahmy ◽  
El sayed A.E. Hamed
Keyword(s):  
Sea Cucumber ◽  
Vibrio Alginolyticus ◽  
Skin Ulceration ◽  
Isolation And Characterization ◽  
Holothuria Atra

Isolation and characterization of a virulence related Vibrio alginolyticus strain Wz11 pathogenic to cuttlefish, Sepia pharaonis

2019 ◽  
Vol 126 ◽  
pp. 165-171 ◽  
Author(s):  
Tengteng Lv ◽  
Tongxiang Song ◽  
Huijie Liu ◽  
Ruibing Peng ◽  
Xiamin Jiang ◽  
...  
Keyword(s):  
Vibrio Alginolyticus ◽  
Sepia Pharaonis ◽  
Isolation And Characterization

scholarly journals Prevalence of Vibrio alginolyticus in Sediment Samples of River and Coastal Areas of Bangladesh

2016 ◽  
Vol 29 (1) ◽  
pp. 1-6 ◽  
Author(s):  
R Siddiqui ◽  
MM Alam ◽  
MN Naser ◽  
Y Otomo ◽  
M Yasmin ◽  
...  
Keyword(s):  
Fish Population ◽  
Vibrio Alginolyticus ◽  
Live Cells ◽  
Economic Damage ◽  
Biochemical Tests ◽  
Marine Animals ◽  
Isolation And Characterization ◽  
Vibrio Pathogens ◽  
Positive Results

Vibrio alginolyticus has been thought to be a halophilic marine bacterium that causes diarrhea, otitis media and wound infection through the consumption of raw or inappropriately cooked sea food. It is one of the main Vibrio pathogens affecting marine animals, such as marine fish, shrimp and shellfish which lead to large economic damage. Although there are reports on the presence of this organism in the coastal area of other countries, not so much work has been done on the isolation and characterization of this species in Bangladesh. The present study was, therefore, undertaken to isolate and characterize V. alginolyticus organisms isolated from the rivers (fresh water) and estuaries (brackish water) of Bangladesh. A total of 9 isolates of Vibrio species were obtained from different water bodies (three from Meghna river, two from Shangu river and four from estuary) and provisionally identified as Vibrio alginolyticus following standard biochemical tests. All these 9 strains showed same pattern of antibiotic resistance to ampicillin, streotomycin, penicillin, but sensitive to nalidixic acid. In the virulence properties test, two isolates showed positive results for toxR gene and none of the isolates showed positive results for tdh gene. Challenge experiments in Singhi fish (Heteropneustes fossi) with the live cells and the culture filtrate prepared from the V. alginolyticus showed high mortality of the fish population. All these studies suggest the presence of pathogenic V. alginolyticus strains in the river water and estuarine bodies of Bangladesh and the extracellular toxin(s) of the V. alginolyticus might be one of the causes for fish mortality.Bangladesh J Microbiol, Volume 29, Number 1, June 2012, pp 1-6

Isolation and characterization of pepsin-solubilized collagen from the integument of sea cucumber (Stichopus vastus )

2012 ◽  
Vol 93 (5) ◽  
pp. 1083-1088 ◽  
Author(s):  
Md Zainul Abedin ◽  
Alias A Karim ◽  
Faiyaz Ahmed ◽  
Aishah A Latiff ◽  
Chee-Yuen Gan ◽  
...  
Keyword(s):  
Sea Cucumber ◽  
Isolation And Characterization

scholarly journals Short Communication Isolation and characterization of microsatellite markers of sea cucumber Stichopus horrens

2015 ◽  
Vol 14 (3) ◽  
pp. 8496-8499 ◽  
Author(s):  
Z.B. Li ◽  
G. Dai ◽  
J.B. Shangguan ◽  
Y.F. Ning ◽  
Y.Y. Li ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Sea Cucumber ◽  
Short Communication ◽  
Isolation And Characterization ◽  
Stichopus Horrens

Frondoside A. A novel triterpene glycoside from the holothurian Cucumariafrondosa

1990 ◽  
Vol 68 (1) ◽  
pp. 11-18 ◽  
Author(s):  
Michel Girard ◽  
Jacqueline Bélanger ◽  
John W. ApSimon ◽  
François-Xavier Garneau ◽  
Christian Harvey ◽  
...  
Keyword(s):  
Double Bond ◽  
Triterpene Glycoside ◽  
Sea Cucumber ◽  
Acetoxy Group ◽  
Chemical Data ◽  
Triterpene Saponins ◽  
Isolation And Characterization ◽  
Physico Chemical

The isolation and characterization of a novel triterpene glycoside from the sea cucumber Cucumariafrondosa, collected from the Gulf of St. Lawrence, are described. Physico-chemical data obtained on the underivatized saponin are presented in support of structure 1 for frondoside A. The structure is characterized by the presence of a branched oligosaccharidic chain composed of five units. The holostane-type aglycone features an endocyclic double bond at position C-7,8 and a β-acetoxy group at C-16. Keywords: triterpene glycoside; Cucumariafrondosa saponins; saponins, triterpene from holothurians; holothurian saponins; echinoderm triterpene saponins.

scholarly journals Evaluation of antibacterial activity and molecular characterization of bacteria from Holothuria atra intestine collected from anthropogenic and non-anthropogenic region in Karimunjawa, Indonesia

2020 ◽  
Vol 21 (7) ◽  
Author(s):  
Bambang Sulardiono ◽  
NINIEK WIDYORINI ◽  
DJOKO SUPRAPTO ◽  
DIAH AYUNINGRUM ◽  
ARIF RAHMAN
Keyword(s):  
Antibacterial Activity ◽  
Molecular Characterization ◽  
Molecular Identification ◽  
Sea Cucumber ◽  
Bacterial Isolates ◽  
Plate Method ◽  
Agar Plug ◽  
16S Rdna Pcr ◽  
Holothuria Atra

Abstract. Sulardiono B, Widyorini N, Suprapto D, Ayuningrum D, Rahman A. 2020. Evaluation of antibacterial activity and molecular characterization of bacteria from Holothuria atra intestine collected from anthropogenic and non-anthropogenic region in Karimunjawa, Indonesia. Biodiversitas 21: 3149-3155. Evaluation of antibacterial activity and molecular characterization of bacteria from the intestine of Holothuria atra is needed in the anthropogenic region of Menjangan Besar, Karimunjawa. The research aims to evaluate antibacterial activity and molecular characterization of bacteria from intestine of Holothuria atra collected from anthropogenic and non-anthropogenic regions in Karimunjawa, Indonesia. Sea cucumber samples were collected at Menjangan Besar waters as anthropogenic region (code of HM) and Alang-alang waters as non-anthropogenics region (code of HA), Karimunjawa National Park, Indonesia. The H. atra sample collection was using purposive sampling method. Examination of the bacteria from isolation until molecular characterisation was done at the Tropical Marine Biotechnology Laboratory, Uiversitas Diponegoro. The isolation process was conducted using spread plate method, followed by bacterial isolates purification using streak plate method. Screening of antibacterial activity using the agar plug method, as well as molecular identification was conducted by 16S rDNA PCR amplification. The results of this study indicated that a total of 26 bacterial strains were successfully isolated from sea cucumber intestine, consisted of eleven bacterial isolates from H. atra in Menjangan Besar waters and the rest fifteen isolates from H. atra in Alang-alang waters. The preliminary assay of antibacterial test showed H. atra in both waters have potential bacterial isolates. Those bacterial isolates with antibacterial activity were HM1.2 and HA1.1, which based on molecular identification, showed isolates HM1.2 and HA1.1 had the closest similarity with Bacillus paramcoides and Vibrio alginolyticus consecutively, with BLAST homology 98% and 99%. The accession number for both isolates were LC550090 and LC550089, respectively.

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