scholarly journals Multiple molecular markers MAGE-1, MAGE-3 and AFP mRNAs expression nested PCR assay for sensitive and specific detection of circulating hepatoma cells: Enhanced detection of hepatocellular carcinoma

2013 ◽  
Vol 14 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Salwa H. Teama ◽  
Sara H.A. Agwa ◽  
Noha A. ElNakeeb ◽  
Manal Abdel Hamid ◽  
Amany M. Maher ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Molecular Markers ◽  
Nested Pcr ◽  
Hepatoma Cells ◽  
Specific Detection ◽  
Pcr Assay

Development of a hemi-nested PCR assay for the specific detection ofMelissococcus pluton

1998 ◽  
Vol 37 (3) ◽  
pp. 165-174 ◽  
Author(s):  
Steven P Djordjevic ◽  
Kerrie Noone ◽  
Lisa Smith ◽  
Michael A Z Hornitzky
Keyword(s):  
Nested Pcr ◽  
Specific Detection ◽  
Pcr Assay

A duplex nested-PCR assay for detection of mud crab reovirus and mud crab dicistrovirus-1

2013 ◽  
Vol 20 (4) ◽  
pp. 808-815
Author(s):  
Di ZHANG ◽  
Keng YANG ◽  
Youlu SU ◽  
Juan FENG ◽  
Zhixun GUO
Keyword(s):  
Nested Pcr ◽  
Mud Crab ◽  
Pcr Assay

Hepatitis C virus (HCV) viremia in patients with hepatocellular carcinoma (HCC) detected by nested-PCR

1991 ◽  
Vol 13 ◽  
pp. S165 ◽  
Author(s):  
J Ruiz ◽  
JI Cuende ◽  
B Sangro ◽  
JI Herrero ◽  
O Belogui ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Nested Pcr

Specific Detection of Chikungunya Virus Using a RT-PCR/Nested PCR Combination

2002 ◽  
Vol 49 (1) ◽  
pp. 49-54 ◽  
Author(s):  
M. PFEFFER ◽  
B. LINSSEN ◽  
M. D. PARKER ◽  
R. M. KINNEY
Keyword(s):  
Nested Pcr ◽  
Chikungunya Virus ◽  
Rt Pcr ◽  
Specific Detection

scholarly journals Neurological disorder in cattle associated with bovine herpesvirus 4

2011 ◽  
Vol 63 (4) ◽  
pp. 828-835 ◽  
Author(s):  
E.A. Costa ◽  
A.C. Vasconcelos ◽  
M.R.Q. Bomfim ◽  
H.B. Amorim ◽  
G.B.L. Lima ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Clinical Course ◽  
Bovine Herpesvirus ◽  
Pcr Assay ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus Type ◽  
Neurological Signs ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 1 ◽  
Herpesvirus 4

A nested PCR assay was used to diagnose bovine encephalitis through herpesviruses including bovine herpesvirus 5 (BHV-5), bovine herpesvirus 1 (BHV-1), Aujeszky's disease virus (SHV-1), and ovine herpesvirus 2 (OHV-2) in 14 fragments of central nervous system (CNS) from cattle that died with neurological signs. In addition, as some samples of bovine herpesvirus type 4 (BHV-4) have been isolated from neural tissue, it was also tested by nested PCR. The cases of encephalitis occurred in isolation at different times of the year and did not present any seasonality. The duration of the clinical course ranged between 1 to 15 days, and in 64.3% of the cases it manifested between 1 to 2 days. The most frequently observed neurological signs were ataxia, recumbency, unsteadiness and inability to stand, opisthotonus, paddling movements, nystagmus and ptyalism. In the nested assay, there was no evidence of: BHV-1, SHV-1 or OHV-2 in the DNA obtained from the CNS in any of the samples. But the presence of BHV-4 was found in all fragments of the CNS in cattle which died presenting neurological signs. Moreover, BHV-5 was found in association with BHV-4 in two of these samples.

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