scholarly journals Neurological disorder in cattle associated with bovine herpesvirus 4

2011 ◽  
Vol 63 (4) ◽  
pp. 828-835 ◽  
Author(s):  
E.A. Costa ◽  
A.C. Vasconcelos ◽  
M.R.Q. Bomfim ◽  
H.B. Amorim ◽  
G.B.L. Lima ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Clinical Course ◽  
Bovine Herpesvirus ◽  
Pcr Assay ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus Type ◽  
Neurological Signs ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 1 ◽  
Herpesvirus 4

A nested PCR assay was used to diagnose bovine encephalitis through herpesviruses including bovine herpesvirus 5 (BHV-5), bovine herpesvirus 1 (BHV-1), Aujeszky's disease virus (SHV-1), and ovine herpesvirus 2 (OHV-2) in 14 fragments of central nervous system (CNS) from cattle that died with neurological signs. In addition, as some samples of bovine herpesvirus type 4 (BHV-4) have been isolated from neural tissue, it was also tested by nested PCR. The cases of encephalitis occurred in isolation at different times of the year and did not present any seasonality. The duration of the clinical course ranged between 1 to 15 days, and in 64.3% of the cases it manifested between 1 to 2 days. The most frequently observed neurological signs were ataxia, recumbency, unsteadiness and inability to stand, opisthotonus, paddling movements, nystagmus and ptyalism. In the nested assay, there was no evidence of: BHV-1, SHV-1 or OHV-2 in the DNA obtained from the CNS in any of the samples. But the presence of BHV-4 was found in all fragments of the CNS in cattle which died presenting neurological signs. Moreover, BHV-5 was found in association with BHV-4 in two of these samples.

Detection of bovine herpesvirus-1 in bovine semen by a nested PCR assay

1993 ◽  
Vol 44 (1) ◽  
pp. 129-139 ◽  
Author(s):  
Martin Wiedmann ◽  
Richard Brandon ◽  
Pat Wagner ◽  
Edward J. Dubovi ◽  
Carl A. Batt
Keyword(s):  
Nested Pcr ◽  
Bovine Herpesvirus ◽  
Pcr Assay ◽  
Bovine Herpesvirus 1 ◽  
Bovine Semen ◽  
Herpesvirus 1

scholarly journals Development of a Polymerase Chain Reaction and Restriction Typing Assay for the Diagnosis of Bovine Herpesvirus 1, Bovine Herpesvirus 2, and Bovine Herpesvirus 4 Infections

2002 ◽  
Vol 14 (4) ◽  
pp. 353-356 ◽  
Author(s):  
Luciana De-Giuli ◽  
Simone Magnino ◽  
Pier Giorgio Vigo ◽  
Iris Labalestra ◽  
Massimo Fabbi
Keyword(s):  
Polymerase Chain Reaction ◽  
Restriction Analysis ◽  
Simultaneous Detection ◽  
Bovine Herpesvirus ◽  
Chain Reaction ◽  
Bovine Herpesvirus 1 ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 1 ◽  
Polymerase Chain ◽  
Herpesvirus 4

A multiplex polymerase chain reaction (PCR) method coupled with a restriction analysis of PCR products (PCR with restriction fragment length polymorphism) was developed for the simultaneous detection of bovine herpesvirus 1, bovine herpesvirus 2, and bovine herpesvirus 4 infections. The specificity, sensitivity, and practical diagnostic applicability of this method were evaluated. This assay may be also adapted to the diagnosis of suid herpesvirus 1 and equine herpesviruses 1 and 3 and could become a powerful diagnostic tool.

EvaGreen-based Multiplex Real-time PCR Assay for Rapid Differentiation of Wild-Type and Glycoprotein E-Deleted Bovine Herpesvirus-1 Strains

2017 ◽  
Vol 28 (4) ◽  
pp. 248-252 ◽  
Author(s):  
Sachin S. Pawar ◽  
Chetan D. Meshram ◽  
Niraj K. Singh ◽  
Mohini Saini ◽  
B. P. Mishra ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bovine Herpesvirus ◽  
Wild Type ◽  
Pcr Assay ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein E ◽  
Herpesvirus 1

scholarly journals First Report on the Detection and Molecular Characterization of Bovine Herpesvirus 1 from a Clinical case of Infectious Bovine Rhinotracheitis in Pakistan

2021 ◽  
Vol 41 (01) ◽  
pp. 160-162
Author(s):  
Aayesha Riaz
Keyword(s):  
Molecular Characterization ◽  
Nested Pcr ◽  
Bovine Herpesvirus ◽  
Nasal Discharge ◽  
Infectious Bovine Rhinotracheitis ◽  
Bovine Herpesvirus 1 ◽  
Nasal Swabs ◽  
Herpesvirus 1 ◽  
First Time

Bovine herpesvirus 1 (BHV-1) is a noteworthy reason for many Cattle/Buffalo diseases. Infectious bovine rhinotracheitis (IBR) is one of the diseases which are caused by the BHV-1. In the present study a cow which was suspected of IBR was examined. The animal was suffering from fever and respiratory distress along with rhinitis (red nose), in appetence, and dyspnea. The nasal mucosa and muzzle were distinctly inflamed, with nasal discharge. DNA from blood samples and nasal swabs were subjected to nested PCR using glycoprotein B gene (gB) Primers. The samples were found positive for BHV-1 gB gene. Sequencing and phylogenetic analysis revealed close similarities with other BHV-1 gB gene sequences. The accession numbers assigned to this pioneer sequences in GenBank are MT449510 (BHV-1-Pak 1) and MT449511 (BHV-1-Pak 2). In this study, we reported for the first time the detection of DNA of BHV-1 through nested PCR assay and molecular characterization of BHV-1 gB gene in Pakistan. This study will be useful in further diagnoses of BHV-1 in Pakistan and in development of BHV-1 vaccine to reduce economical losses due to IBR

scholarly journals Novel herpesviruses of Suidae: indicators for a second genogroup of artiodactyl gammaherpesviruses

2004 ◽  
Vol 85 (4) ◽  
pp. 857-862 ◽  
Author(s):  
Bernhard Ehlers ◽  
Stewart Lowden
Keyword(s):  
Novel Species ◽  
Bovine Herpesvirus ◽  
South East Asia ◽  
The Novel ◽  
Porcine Cytomegalovirus ◽  
Domestic Pigs ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 1 ◽  
Phacochoerus Africanus ◽  
Herpesvirus 4

Five novel herpesviruses were identified in suid species from Africa (common warthog, Phacochoerus africanus) and South-East Asia (bearded pig, Sus barbatus; babirusa, Babyrousa babyrussa) by detection and analysis of their DNA polymerase genes. Three of the novel species, P. africanus cytomegalovirus 1, P. africanus lymphotropic herpesvirus 1 (PafrLHV-1) and S. barbatus lymphotropic herpesvirus 1 (SbarLHV-1), were closely related to known beta- (porcine cytomegalovirus) and gammaherpesviruses [porcine lymphotropic herpesvirus (PLHV) 1 and 3] of domestic pigs. In contrast, two novel species, S. barbatus rhadinovirus 1 (SbarRHV-1) and Babyrousa babyrussa rhadinovirus 1 (BbabRHV-1), were more closely related to a ruminant gammaherpesvirus, bovine herpesvirus 4 (BoHV-4), than to the porcine gammaherpesviruses PLHV-1, -2, -3, PafrLHV-1 and SbarLHV-1. SbarRHV-1, BbabRHV-1 and BoHV-4 were therefore tentatively assigned to a novel genogroup of artiodactyl gammaherpesviruses. This latter genogroup may also contain an as yet undiscovered gammaherpesvirus of domestic pigs, thereby adding a concern to their use in xenotransplantation.

An international inter-laboratory ring trial to evaluate a real-time PCR assay for the detection of bovine herpesvirus 1 in extended bovine semen

2008 ◽  
Vol 126 (1-3) ◽  
pp. 11-19 ◽  
Author(s):  
Jianning Wang ◽  
Joseph O’Keefe ◽  
Della Orr ◽  
Leo Loth ◽  
Malcolm Banks ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bovine Herpesvirus ◽  
Pcr Assay ◽  
Bovine Herpesvirus 1 ◽  
Bovine Semen ◽  
Ring Trial ◽  
Herpesvirus 1

scholarly journals PREVALENCE OF HERPESVIRUS 1 IN SMALL HERDS IN SOUTHERN BAČKA AND SREM DISTRICT

2008 ◽  
Vol 1 (1) ◽  
pp. 18-31
Author(s):  
Sava Lazić ◽  
Tamaš Petrović ◽  
Diana Lupulović ◽  
Dejan Bugarski ◽  
Ivan Pušić ◽  
...  
Keyword(s):  
Bovine Herpesvirus ◽  
Control Measures ◽  
Economic Losses ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus Type ◽  
Starting Point ◽  
Bovine Herpesvirus Type 1 ◽  
Herpesvirus 1 ◽  
Low Prevalence

Bovine herpesvirus type 1 infection (IBR/IPV virus) presents the most spread infection in nowadays cattle breeding. Bovine herpesvirus-1 (BHV-1) may be an agent of serious health disorders causing great economic losses. Since the largest number of cattle in Southern Baåka and Srem district are raised in small herds (what may considerably influence effective implementation of program for control and eradication of BHV-1 infection) it was necessary to investigate the prevalence of BHV-1 infection in the cattle population in this area, what was the aim of this work. Detection and estimation of the presence and prevalence of BHV-1 was done by detection of specific antibodies against BHV-1 in sera samples of cattle raised individually or in small herds (up to 20 animals) collected during the State Program of Disease Control in 2005 and 2006. The sampling was randomly carried out taking care that the samples from all the settlements and municipalities in Southern Bačka and Srem were present. A total of 16,610 samples was collected. Animals seropositive for BHV-1 were detected in all municipalities, but not in all settlements. The highest percent of seropositive animals was found in Beočin municipality(27.27%), Titel municipality (27.16%), Žabalj (22.45%) and Stara Pazova municipality (22.15%). The lowest prevalence was in Bački Petrovac (8.16%) and Temerin municipalities (9.68%). In other municipalities the prevalence ranged between 10% and 20%. From the obtained results it can be concluded that BHV-1 infection was not present in 10 settlements in Southern Bačka and 14 settlements in Srem district. In other settlements (151) the percentage of seropositive animals ranged from 1 to 50%. However, if we analyze the obtained results in the area, it may be concluded that the prevalence is almost the same: in Srem district there were 18.42% and in Southern Bačka 18.79% seropositive animals. Low prevalence was detected in the majority of settlements. The data that in 24 settlements in both districts there were no seropositive animals His an encouraging information. This points on a possibility of easier and faster implementation of control measures and eradication of BHV-1 infection. The results obtained in serology examination provide us an insight in immunology status of small herds in Southern Bačka and Srem districts, what is a starting point in designing and implementing necessary measures with the aim to prevent cases of this disease, as well as its spreading and eradication.

Validation of a real-time PCR assay for the detection of bovine herpesvirus 1 in bovine semen

2007 ◽  
Vol 144 (1-2) ◽  
pp. 103-108 ◽  
Author(s):  
Jianning Wang ◽  
Joseph O’Keefe ◽  
Della Orr ◽  
Leo Loth ◽  
Malcolm Banks ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bovine Herpesvirus ◽  
Pcr Assay ◽  
Bovine Herpesvirus 1 ◽  
Bovine Semen ◽  
Herpesvirus 1

scholarly journals Bovine herpesvirus 5 encodes a unique pattern of microRNAs compared with bovine herpesvirus 1

2014 ◽  
Vol 95 (3) ◽  
pp. 671-678 ◽  
Author(s):  
Qi Tang ◽  
Yi-Quan Wu ◽  
Dong-Sheng Chen ◽  
Qing Zhou ◽  
Huan-Chun Chen ◽  
...  
Keyword(s):  
Bovine Herpesvirus ◽  
Mature Mirnas ◽  
Productive Infection ◽  
Reference Sequence ◽  
Cell Protein ◽  
Bovine Herpesvirus 1 ◽  
Stem Loop ◽  
Herpesvirus Type ◽  
Mirna Genes ◽  
Herpesvirus 1

Bovine herpesvirus type 5 (BoHV-5) and bovine herpesvirus 1 (BoHV-1) are two closely related viruses. However, BoHV-5 is responsible for fatal meningitis in calves, while BoHV-1 is associated with infectious rhinotracheitis in cattle, and the mechanism by which the two viruses cause different symptoms is not well understood. In this study, we identified 11 microRNA (miRNA) genes, encoded by the BoHV-5 genome, that were processed into 16 detectable mature miRNAs in productive infection as determined by deep sequencing. We found that 6 out of 16 miRNA genes were present as two copies in the internal repeat and terminal repeat regions, resulting in a total of 17 miRNA-encoding loci distributed in both DNA strands. Surprisingly, BoHV-5 shared only one conservative miRNA with BoHV-1, which was located upstream of the origin of replication. Furthermore, in contrast to BoHV-1, no miRNAs were detected in the unique short region and locus within or near the bovine infected-cell protein 0 and latency-related genes. Variations in both the 5′ and 3′ ends of the reference sequence were observed, resulting in more than one isoform for each miRNA. All of the 16 miRNAs were detectable by stem–loop reverse transcriptase-PCR. The miRNAs with high read numbers were subjected to Northern blot analysis, and all pre-miRNAs and one mature miRNA were detected. Collectively, the data suggest that BoHV-5 encodes a different pattern of miRNAs, which may regulate the life cycle of BoHV-5 and might account for the different pathogenesis of this virus compared with BoHV-1.

scholarly journals Outbreak Control and Clinical, Pathological, and Epidemiological Aspects and Molecular Characterization of a Bovine Herpesvirus Type 5 on a Feedlot Farm in São Paulo State

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Jane Megid ◽  
Acácia Ferreira Vicente ◽  
Camila Michele Appolinario ◽  
Susan Dora Allendorf ◽  
Mateus de Souza Ribeiro Mioni ◽  
...  
Keyword(s):  
Virus Transmission ◽  
Bovine Herpesvirus ◽  
Sao Paulo ◽  
São Paulo ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus Type ◽  
Paulo State ◽  
Outbreak Control ◽  
São Paulo State ◽  
Herpesvirus 1

This paper describes the control, epidemiological, pathological, and molecular aspects of an outbreak of meningoencephalitis in calves due to bovine herpesvirus 5 at a feedlot with 540 animals in São Paulo State, Brazil. The introduction of new animals and contact between the resident animals and the introduced ones were most likely responsible for virus transmission. Bovine herpesvirus 1 vaccine was used, resulting in the efficacy of the outbreak control, although two bovine herpesvirus 1 positive animals, vaccinated and revaccinated, presented meningoencephalitis, thereby characterizing vaccinal failure.

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