The External Aortic Wrapped Graft Restrain the Growth of the Ascending Aorta in a Large Animal Model: Functional, Molecular and Morphological Changes After Minimally Invasive Aortic Root Support: Six-month Follow-up

Objective Current minimally invasive techniques for ablation in superficial venous reflux are limited to thermal based systems requiring tumescent anesthesia, non-thermal chemical sclerosants and permanent glue implantation. The aim of this feasibility study was to determine the safety and efficacy of a novel mechanical-only ablation (MOA) device called EnVena, in a recognised large animal model with chronic follow up. Methods Venous ablation of six lateral saphenous veins in three sheep was performed using the EnVena device. Luminal patency and vein wall fibrosis were evaluated by histologic analysis at 51 and 89 days. Results All treated veins demonstrated fibrotic occlusion in contiguous segments at 51 and 89 days on histological analysis. From 45 consecutive segments spanning the treatment length across the six treated veins, 26 (57%) were fully occluded, 7 (16%) were impinged or partially occluded and 12 (27%) were open. There were no device related complications during the follow up period. Conclusions A purely mechanical approach to superficial venous ablation demonstrated safety and efficacy in a recognized large animal model based on histological findings.

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First-time systematic postoperative clinical assessment of a minimally invasive approach for lumbar ventrolateral vertebroplasty in the large animal model sheep

The Spine Journal ◽

10.1016/j.spinee.2016.06.015 ◽

2016 ◽

Vol 16 (10) ◽

pp. 1263-1275 ◽

Cited By ~ 13

Author(s):

Matthias Bungartz ◽

Stefan Maenz ◽

Elke Kunisch ◽

Victoria Horbert ◽

Long Xin ◽

...

Keyword(s):

Animal Model ◽

Minimally Invasive ◽

Clinical Assessment ◽

Large Animal Model ◽

Large Animal ◽

Minimally Invasive Approach ◽

Invasive Approach ◽

First Time

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Functional Growth in Tissue-Engineered Living, Vascular Grafts: Follow-Up at 100 Weeks in a Large Animal Model

Circulation ◽

10.1161/circulationaha.105.001172 ◽

2006 ◽

Vol 114 (1_suppl) ◽

pp. I-159-I-166 ◽

Cited By ~ 108

Author(s):

S. P. Hoerstrup

Keyword(s):

Animal Model ◽

Vascular Grafts ◽

Large Animal Model ◽

Large Animal

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Sub-acute Safety and Efficacy Evaluation of Single versus Double Treatment Cycles of a Monopolar Radiofrequency Catheter-Based Renal Nerve Ablation and its Chronic Evolution in a Large Animal Model

Journal of Advanced Therapies and Medical Innovation Sciences ◽

10.17987/jatamis.v1i0.346 ◽

2016 ◽

Vol 1 ◽

Author(s):

Bradley Hubbard ◽

Atsushi Sakaoka ◽

Irena Brants ◽

Krista Dillon ◽

Dane Brady ◽

...

Keyword(s):

Animal Model ◽

Large Animal Model ◽

Large Animal ◽

Efficacy Evaluation ◽

Renal Nerve ◽

Treatment Groups ◽

Renal Nerve Ablation ◽

Circumferential Extent ◽

Human Use

Aims:We aimed to evaluate the transcatheter renal denervation(RDN) effects delivered by a mono-electrode catheter in a large animal model including safety implications of delivery of one cycle versus two cycles of ablations.Methods and Results:18 animals underwent bilateral RDN; 4 untreated naïve swine were enrolled as controls for norepinephrine levels(NE) only. Animals recieved 120-second (follow up-7,30 and 90 days) or 240-second cycles ablations (follow up-7 days). Norepinephrine evaluation, histology and immunohistochemistry evaluation was performed. No luminal obstruction was observed at follow up. A 70% decrease in NE levels (76.68±57.87ng/g) was observed at 7 days, 81% at 30 days(49.05±45.81ng/g), and 51% at 90 days(12.7±73.2 ng/g) compared to naïve controls(254.1±54.1ng/g;p<0.05). Histologically, the thermal effect extended to a complete circumferential involvement with a depth ~8mm. The primary histological feature at 7 days was nerve necrosis and distal atrophy; at 30 days, necrosis was replaced by healing changes of fibrosis. Neuromatous regeneration was apparent at 30 days at RF treated levels. At 90 days these features progressed to become more conspicuous. There were no appreciable differences in depth and circumferential extent of RF injury between one and two cycle treatment groups.Conclusion:RDN performed with a mono-electrode catheter (Iberis) appears to be safe following single or double-cycle RF ablation.NE decrease following RDN was demonstrated at 7, 30, and 90 days compared to naïve controls, suggesting efficient nerve ablation with the device as intended for human use.

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Gross Morphological Changes of the Spinal Cord Immediately After Surgical Decompression in a Large Animal Model of Traumatic Spinal Cord Injury

Spine ◽

10.1097/brs.0b013e3182553d1d ◽

2012 ◽

Vol 37 (15) ◽

pp. E890-E899 ◽

Cited By ~ 16

Author(s):

Claire F. Jones ◽

Peter A. Cripton ◽

Brian K. Kwon

Keyword(s):

Spinal Cord ◽

Spinal Cord Injury ◽

Animal Model ◽

Morphological Changes ◽

Surgical Decompression ◽

Large Animal Model ◽

Traumatic Spinal Cord Injury ◽

Large Animal ◽

Cord Injury

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Long-Term Cell Tracking following Local Injection of Mesenchymal Stromal Cells in the Equine Model of Induced Tendon Disease

Cell Transplantation ◽

10.3727/096368916x692104 ◽

2016 ◽

Vol 25 (12) ◽

pp. 2199-2211 ◽

Cited By ~ 24

Author(s):

Janina Burk ◽

Dagmar Berner ◽

Walter Brehm ◽

Aline Hillmann ◽

Carolin Horstmeier ◽

...

Keyword(s):

Animal Model ◽

Mesenchymal Stromal Cells ◽

Rhodamine B ◽

Stromal Cells ◽

Cell Tracking ◽

Local Application ◽

Large Animal Model ◽

Large Animal

Tendon disease has been treated with multipotent mesenchymal stromal cells (MSCs) in the equine large-animal model with promising success. The aim of this study was to gain more insight into the fate and biodistribution of MSCs after local application into tendon lesions by long-term cell tracking in this large-animal model. Superficial digital flexor tendon lesions were induced in all limbs in six horses and injected with 10 × 10 6 Molday ION Rhodamine B™-labeled MSCs suspended in serum or serum alone. Follow-up was performed using low-field magnetic resonance imaging (MRI), flow cytometry, and histology. Cell tracking based on the hypointense artifacts induced by the superparamagnetic iron oxide (SPIO) labeling agent in MRI as well as based on Rhodamine B fluorescence was feasible. However, Prussian blue staining for assessment of histology was not entirely specific for SPIO. Labeled cells could be traced at their injection site by MRI as well as histology for the whole follow-up period of 24 weeks. Although the numbers of labeled cells within the injected tendon lesions decreased over time, part of the applied cells appeared to remain viable and integrated within the injured tissue. Furthermore, small numbers of labeled cells were identified in peripheral blood within the first 24 h after cell injection and could also be found until week 24 within the contralateral control tendon lesions that had been injected with serum. The present findings unveil details on MSC biodistribution and persistence after their local application, which are of clinical relevance with regard to MSC safety and mechanisms of action.

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