Improving the decolorization activity of Bacillus pumilus W3 CotA-laccase to Congo Red by rational modification

2021 ◽  
pp. 109977
Author(s):  
Na Yan ◽  
Hui Ma ◽  
Chun-Xue Yang ◽  
Xiang-Ru Liao ◽  
Zheng-Bing Guan
Keyword(s):  
Congo Red ◽  
Bacillus Pumilus ◽  
Cota Laccase

Enhancing the decolorization activity of Bacillus pumilus W3 CotA-laccase to Reactive Black 5 by site-saturation mutagenesis

2020 ◽  
Vol 104 (21) ◽  
pp. 9193-9204
Author(s):  
Hui Ma ◽  
Kai-Zhong Xu ◽  
Ya-Jing Wang ◽  
Na Yan ◽  
Xiang-Ru Liao ◽  
...  
Keyword(s):  
Bacillus Pumilus ◽  
Saturation Mutagenesis ◽  
Reactive Black 5 ◽  
Site Saturation ◽  
Cota Laccase

Enhancement in catalytic activity of CotA-laccase from Bacillus pumilus W3 via site-directed mutagenesis

2020 ◽  
Vol 129 (4) ◽  
pp. 405-411 ◽  
Author(s):  
Kai-Zhong Xu ◽  
Hao-Ran Wang ◽  
Ya-Jing Wang ◽  
Jing Xia ◽  
Hui Ma ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Bacillus Pumilus ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Cota Laccase

Improving the catalytic efficiency of Bacillus pumilus CotA-laccase by site-directed mutagenesis

2016 ◽  
Vol 101 (5) ◽  
pp. 1935-1944 ◽  
Author(s):  
Yu Chen ◽  
Quan Luo ◽  
Wen Zhou ◽  
Zeng Xie ◽  
Yu-Jie Cai ◽  
...  
Keyword(s):  
Bacillus Pumilus ◽  
Catalytic Efficiency ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Cota Laccase

Inducement Expression and Synthetic Dyes Decolourization Capacity of CotA

2011 ◽  
Vol 236-238 ◽  
pp. 2344-2348
Author(s):  
Ning Zhang ◽  
Min Zhao ◽  
Chun Lei Wang ◽  
Guang Ying Hui Du
Keyword(s):  
Escherichia Coli ◽  
Enzyme Activity ◽  
Bacillus Subtilis ◽  
Crystal Violet ◽  
Congo Red ◽  
Induction Time ◽  
Enzyme Production ◽  
Final Concentration ◽  
Synthetic Dyes ◽  
Cota Laccase

Bacillus subtilis cotA gene which was designed According to GenBank. cotA gene was transformed to Escherichia coli BL21(DE3). The recombined CotA protein was expressed by IPTG induced method. The CotA protein was stained red by syringaldazine after Native-PAGE.The effects of the condition of induction expression on enzyme production of recombinant Escherichia coli were investigated. The result showed that the highest enzyme activity of recombinant CotA could be achieved under the following conditions: the concentration of IPTG was 1.0 mmol L-1, IPTG was added to the culture given a final concentration of 1.0 mol L-1 when the OD600 of culture reached 1.0, induction time was 12 h at 25°C. The CotA laccases could decolorize Remazol brilliant blue R (RBBR) and Congo red with 87% and Isatin and Crystal Violet with 55%. The result indicated that the CotA laccase had the potential to be industrial enzyme.

The Use of Radioiodinated Congo Red in the Study of Amyloidosis

1963 ◽  
Vol 03 (01) ◽  
pp. 25-38
Author(s):  
Manuel Tubis ◽  
William Blahd ◽  
John Endow
Keyword(s):  
Rheumatoid Arthritis ◽  
Multiple Myeloma ◽  
Comparative Studies ◽  
Congo Red ◽  
Half Time ◽  
Positive Correlation ◽  
Autopsy Findings ◽  
Percentage Removal

SummaryA study of the removal of I131-labeled Congo red from the blood of amyloid, non-amyloid, multiple myeloma, rheumatoid arthritis and other patients is presented. The percentage removal of the labeled dye shows the same variation reported by many other workers using Bennhold’s test and its modifications.However, there seems to be a positive correlation between the percentage removal of the labeled dye and the presence of amyloid as revealed by biopsy and autopsy. The half-time of disappearance is also correlated with the amyloidosis.The availability of the I131-labeled dye permits the use of very small weights of the dye thereby drastically reducing the possibility of toxic and sometimes fatal reactions encountered with the unlabeled dye. The I131 present permits easy quantitation of the dye in the blood without separation of plasma and obviates the need of fasting. It also permits external counting and scanning of deposits in the organs containing the dye.The availability and use of the labeled dye may stimulate more comparative studies of the removal of the dye from the blood correlated with biopsy and autopsy findings.

Effect of Congo Red on Blood Platelets and Leucocytes of Rabbits and Cats

1971 ◽  
Vol 26 (03) ◽  
pp. 488-492 ◽  
Author(s):  
Th B. Tschopp ◽  
H.-R Baumgartner ◽  
A Studer
Keyword(s):  
Fatty Acids ◽  
Congo Red ◽  
Platelet Rich Plasma ◽  
Blood Platelets ◽  
Severe Thrombocytopenia ◽  
Ultrastructural Alterations ◽  
Indirect Mechanism

SummaryIn rabbits and cats Congo red administered intravenously causes severe thrombocytopenia and ultrastructural alterations of platelets and leucocytes, similar to those produced by some fatty acids and endotoxin. Transient leucopenia is followed by leucocytosis. In contrast, incubation of Congo red in citrated blood or platelet rich plasma has no effect. Therefore, an indirect mechanism is postulated to explain the in vivo effect of Congo red.

An effective method for determining the retention and distribution of cellulose nanofibrils in paper handsheets by dye labeling

TAPPI Journal ◽  
2018 ◽  
Vol 17 (03) ◽  
pp. 157-164 ◽  
Author(s):  
Shengdan Wang ◽  
Wenhua Gao ◽  
Kefu Chen ◽  
Jinsong Zeng ◽  
Jun Xu ◽  
...  
Keyword(s):  
Correlation Coefficient ◽  
Congo Red ◽  
Kraft Pulp ◽  
Cellulose Nanofibrils ◽  
Standard Curve ◽  
Congo Red Dye ◽  
Mechanical Disintegration ◽  
Softwood Kraft Pulp ◽  
White Water ◽  
Red Dye

Cellulose nanofibrils (CNF) were prepared by cellulase in conjunction with mechanical disintegration from the bleached softwood kraft pulp and labelled by Congo red dye. The labelled CNF were used to investigate the retention and distribution of CNF in paper handsheets. The retention of the labelled CNF was obtained by measuring the absorbance of white water using an ultraviolet-visible spectrophotometer. The results showed that this method for measuring the retention was rapid, feasible, and sensitive, owing to the high correlation coefficient R2 (0.9993) of the standard curve. The labelled CNF showed even distribution in paper handsheets. The colorimetric values of paper handsheets were explored with a residual ink analyzer.

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