“Gene-to-Metabolite” network prompted engineering of rosmarinic acid biosynthetic pathway in Salvia miltiorrhiza hairy root cultures

2014 ◽  
Vol 6 (6) ◽  
pp. 729
Author(s):  
Ying Xiao ◽  
Lei Zhang ◽  
Wansheng Chen
Keyword(s):  
Hairy Root ◽  
Rosmarinic Acid ◽  
Biosynthetic Pathway ◽  
Salvia Miltiorrhiza ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Metabolite Network

scholarly journals The c4h, tat, hppr and hppd Genes Prompted Engineering of Rosmarinic Acid Biosynthetic Pathway in Salvia miltiorrhiza Hairy Root Cultures

PLoS ONE ◽  
2011 ◽  
Vol 6 (12) ◽  
pp. e29713 ◽  
Author(s):  
Ying Xiao ◽  
Lei Zhang ◽  
Shouhong Gao ◽  
Saengking Saechao ◽  
Peng Di ◽  
...  
Keyword(s):  
Hairy Root ◽  
Rosmarinic Acid ◽  
Biosynthetic Pathway ◽  
Salvia Miltiorrhiza ◽  
Hairy Root Cultures ◽  
Root Cultures

Metabolic engineering tanshinone biosynthetic pathway in Salvia miltiorrhiza hairy root cultures

2011 ◽  
Vol 13 (3) ◽  
pp. 319-327 ◽  
Author(s):  
Guoyin Kai ◽  
Hui Xu ◽  
Congcong Zhou ◽  
Pan Liao ◽  
Jianbo Xiao ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Hairy Root ◽  
Biosynthetic Pathway ◽  
Salvia Miltiorrhiza ◽  
Hairy Root Cultures ◽  
Root Cultures

Lithospermic acid B is more responsive to silver ions (Ag+) than rosmarinic acid in Salvia miltiorrhiza hairy root cultures

2009 ◽  
Vol 30 (1) ◽  
pp. 33-40 ◽  
Author(s):  
Ying Xiao ◽  
Shouhong Gao ◽  
Peng Di ◽  
Junfeng Chen ◽  
Wansheng Chen ◽  
...  
Keyword(s):  
Hairy Root ◽  
Rosmarinic Acid ◽  
Salvia Miltiorrhiza ◽  
Silver Ions ◽  
Dry Weight ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Feeding Experiments ◽  
Abiotic Elicitor ◽  
Gene Transcripts

LAB (lithospermic acid B) is a dimer of RA (rosmarinic acid) and has been suggested to be derived from RA, but the detailed biosynthesis process has not yet been identified. The accumulation of RA has been intensively investigated in the plant species of Boraginaceae and Lamiaceae. In the present study, we report that silver ions (Ag+; 15 μM), an abiotic elicitor, did not stimulate RA accumulation but dramatically enhanced LAB from approx. 5.4% to 18.8% of dry weight in Salvia miltiorrhiza hairy root cultures, and the rise in LAB was found to be coincident with the decline of RA content at each time point after treatment. Meanwhile, a profiling analysis of genes and metabolites (intermediates) involved in the RA synthesis pathway was performed; the result indicated that several gene transcripts and metabolite accumulations show temporal changes in abundance consistent with LAB production. Thus a potential (putative) biosynthetic route from RA to LAB was presumed, which was suggested to be significantly activated by Ag+ in S. miltiorrhiza hairy root cultures. Further intermediate monitoring and compound feeding experiments were performed to rank the strength of this hypothesis. Our study, for the first time, provides evidence that RA is a precursor leading to LAB synthesis.

scholarly journals La Dramaticaly Enhances the Accumulation of Tanshinones in Salvia Miltiorrhiza Hairy Root Cultures

2012 ◽  
Vol 2 (1) ◽  
Author(s):  
Jie Zhou ◽  
Lei Fang ◽  
Xiao Wang ◽  
Lanping Guo ◽  
Luqi Huang
Keyword(s):  
Hairy Root ◽  
Salvia Miltiorrhiza ◽  
Hairy Root Cultures ◽  
Root Cultures

The identification and quantitative determination of rosmarinic acid and salvianolic acid B in hairy root cultures of Dracocephalum forrestii W.W. Smith

2016 ◽  
Vol 91 ◽  
pp. 125-131 ◽  
Author(s):  
Izabela Weremczuk-Jeżyna ◽  
Ewa Skała ◽  
Monika A. Olszewska ◽  
Anna K. Kiss ◽  
Ewa Balcerczak ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Hairy Root ◽  
Rosmarinic Acid ◽  
Salvianolic Acid B ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Salvianolic Acid

Rosmarinic acid production in hairy root cultures of Agastache rugosa Kuntze

2007 ◽  
Vol 24 (7) ◽  
pp. 969-972 ◽  
Author(s):  
Sook Young Lee ◽  
Hui Xu ◽  
Yong Kyung Kim ◽  
Sang Un Park
Keyword(s):  
Hairy Root ◽  
Rosmarinic Acid ◽  
Acid Production ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Agastache Rugosa
Sign in / Sign up

Export Citation Format

Share Document