Babesia gibsoni: Serodiagnosis of infection in dogs by an enzyme-linked immunosorbent assay with recombinant BgTRAP

2008 ◽  
Vol 118 (4) ◽  
pp. 555-560 ◽  
Author(s):  
Youn-Kyoung Goo ◽  
Honglin Jia ◽  
G. Oluga Aboge ◽  
M. Alaa Terkawi ◽  
Ken Kuriki ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Babesia Gibsoni

scholarly journals Serodiagnosis of Babesia gibsoni Infection in Dogs by an Improved Enzyme-Linked Immunosorbent Assay with Recombinant Truncated P50

2004 ◽  
Vol 66 (12) ◽  
pp. 1517-1521 ◽  
Author(s):  
Rodolfo A. VERDIDA ◽  
Olgga A. HARA ◽  
Xuenan XUAN ◽  
Shinya FUKUMOTO ◽  
Ikuo IGARASHI ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Babesia Gibsoni

SERODIAGNOSIS OF CANINE BABESIA GIBSONI INFECTION BY ENZYME-LINKED IMMUNOSORBENT ASSAY WITH RECOMBINANT P50 EXPRESSED IN ESCHERICHIA COLI

2004 ◽  
Vol 90 (2) ◽  
pp. 387-391 ◽  
Author(s):  
Shinya Fukumoto ◽  
Yukiko Sekine ◽  
Xuenan Xuan ◽  
Ikuo Igarashi ◽  
Chihiro Sugimoto ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Babesia Gibsoni

Molecular characterization of a novel 32-kDa merozoite antigen ofBabesia gibsoniwith a better diagnostic performance by enzyme-linked immunosorbent assay

Parasitology ◽  
2007 ◽  
Vol 134 (9) ◽  
pp. 1185-1194 ◽  
Author(s):  
G. O. ABOGE ◽  
H. JIA ◽  
K. KURIKI ◽  
J. ZHOU ◽  
Y. NISHIKAWA ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Diagnostic Performance ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protozoan Parasites ◽  
Babesia Gibsoni ◽  
Gene Encoding ◽  
Reading Frame ◽  
Immunodominant Antigen ◽  
Gst Fusion Protein

SUMMARYWe cloned and expressed a novel gene encoding a 32-kDa merozoite protein ofBabesia gibsoni(BgP32). The length of nucleotide sequence of the cDNA was 1464 bp with an open reading frame of 969 bp. The truncated recombinant BgP32 (rBgP32) without a signal peptide and C-terminal hydrophobic sequence was expressed inEscherichia colias a soluble glutathione-S-transferase (GST) fusion protein. Western blotting demonstrated that the native protein was 32-kDa, consistent with molecular weight of the predicted mature polypeptide. Enzyme-linked immunosorbent assay (ELISA) using rBgP32 detected specific antibodies from 8 days to 541 days post-infection in the sequential sera from a dog experimentally infected withB. gibsoni. Moreover, the antigen did not cross-react withB. canissubspecies and closely related protozoan parasites, indicating that rBgP32 is a specific diagnostic antigen. Analysis of 47 sera taken from dogs with anaemic signs revealed that rBgP32 detected a higher proportion ofB. gibsoniseropositive samples (77%) than its previously identified rBgP50 (68%) homologue. These results indicate that the BgP32 is a novel immunodominant antigen ofB. gibsoni, and rBgP32 might be useful for diagnosis ofB. gibsoniinfection.

A novel 57-kDa merozoite protein of Babesia gibsoni is a prospective antigen for diagnosis and serosurvey of canine babesiosis by enzyme-linked immunosorbent assay

2007 ◽  
Vol 149 (1-2) ◽  
pp. 85-94 ◽  
Author(s):  
Gabriel Oluga Aboge ◽  
Honglin Jia ◽  
Mohamad Alaa Terkawi ◽  
Younkyoung Goo ◽  
Ken Kuriki ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Canine Babesiosis ◽  
Babesia Gibsoni
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