canine babesiosis
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2021 ◽  
Vol 12 (4) ◽  
pp. 670-675
Author(s):  
O. A. Dubova ◽  
D. V. Feshchenko ◽  
T. I. Bakhur ◽  
A. A. Dubovyi ◽  
O. A. Zghozinska ◽  
...  

Disseminated intravascular coagulation (DIC) syndrome is the main defining process in the pathogenetic axis of complications in canine babesiosis. The involvement of the spleen with further irreversible changes in the organ largely determines the severity of the animal’s condition after spontaneous babesiosis. The work presented here aimed to determine the role of the DIC syndrome as a triggering factor for lesions of the spleen. Clinical and laboratory studies (haematological, biochemical, hemodynamic) have been carried out. Pathological studies of the removed spleen were carried out by histological methods using universal and specific staining. After suffering acute spontaneous babesiosis, the development of hypersplenism and splenomegaly was found in dogs. The diagnosis was confirmed haematologically by the detected cytopenia, normochromic type anaemia. An additional parameter was a significantly increased erythrocyte sedimentation rate. The biochemical profile indicated the development of bilirubinaemia due to the conjugated fraction, hyperfermentation of transaminases, hypoalbuminemia, which reflected the development of hepatitis and liver failure. Markers of DIC syndrome in laboratory studies are represented by reliable hypofibrinogenemia, increased level of fibrinogen/fibrin degradation products, including D-dimer, and soluble fibrin monomer complexes. The multidirectional indices of coagulation tests (activated partial thromboplastin and prothrombin time) made it possible to classify the stage of “consumption coagulopathy” of the DIC syndrome. The haemodynamic parameters of the sick dogs were characterized by a significant deficit in the circulating blood volume. Together with the indicators of the “consumption coagulopathy” stage of the DIC syndrome, the hemodynamic indexes indicate a moderate degree of shock stage II – the stable reversibility, but the magnitude of the circulating blood volume deficit determines the tendency towards shock irreversibility. Histological studies have established a significant proliferation of the stromal elements of the organ, the formation of specific complexes of vessels with sinuses, clogging with blood clots, and the organ's parenchyma dystrophy. Such changes characterize complete splenomegaly, which is based on the organo-pathology of the DIC syndrome. The deposition of “old” fibrin in the connective tissue structures of the spleen indicates that DIC syndrome continues throughout the entire period of hyperplastic changes in the organ. The presence of hyalinosis in blood vessel walls of the spleen parenchyma determines irreversible changes in them. Thus, DIC syndrome is the basis for splenomegaly development in dogs after acute spontaneous babesiosis. It is confirmed by laboratory blood tests and histologically by the presence of fibrin thrombi in the structures of the organ, which determine the organopathology of the syndrome. The information obtained serves to expand the concepts of the pathogenesis of blood protozoal disease, define the high risk of complications that can become fatal for the health and life of animals.


2021 ◽  
Author(s):  
Ismail Thoya Ngoka ◽  
Kevin Mbogo ◽  
Martina Kyallo ◽  
David Obiero ◽  
Roger Pelle

Abstract The genus Babesia has more than 100 species that are transmitted by ticks with some being zoonotic. They can infect humans, livestock, and wildlife. Although canine babesiosis occurs locally, published studies on the species involved are limited. Babesia parasites cause severe disease in dogs which can be fatal. Drawbacks of the current control methods necessitate vaccine development. The study objective was to identify the Babesia species infecting dogs from three Kenyan counties; Nairobi, Mombasa, Nakuru and determine their phylogenetic relationship. This will enable improved control and rule out zoonotic potential. The study period was October 2018 to November 2019.The study design was descriptive and sampling opportunistic. One hundred and forty-three dogs were sampled. From whole blood, total DNA was extracted using the TanBead extractor followed by PCR amplification targeting Babesia 18S rRNA. Positive samples were purified and sequenced using the Sanger Dideoxy method.CLC Genomics Workbench, GenBank™ and BLASTn™ on NCBI were used for sequence processing and analysis. Geneious prime™ was used for multiple sequence alignment and phylogenetic analysis. The overall prevalence of Babesia canis was 9.0% (95% CI: 4.37–13.81). Two out of 13 positive samples (2/13) were identified as Babesia canis vogeli, with a prevalence of 1.4% (95% CI: 1.38–14.2, n = 143) while 11/13 were identified as Babesia canis rossi, with a prevalence of 7.69% (95% CI: 3.3–12, n = 143). The Babesia rossi sequences identified were closely related to sequences from black-backed jackals, while the Babesia vogeli ones were related to sequences from a pet cat in China. Babesia rossi which causes severe canine babesiosis was identified in 84.6% of the positive samples, immediate and aggressive clinical intervention is necessary. The possible sylvatic cycle of Babesia rossi and low levels of infections by Babesia vogeli should inform pertinent control measures.


2021 ◽  
Vol 12 ◽  
Author(s):  
Josipa Kuleš ◽  
Ivana Rubić ◽  
Blanka Beer Ljubić ◽  
Petra Bilić ◽  
Renata Barić Rafaj ◽  
...  

Canine babesiosis is a tick-borne disease with a worldwide distribution, caused by the haemoprotozoan parasites of the genus Babesia. One of the most prevalent complication is acute kidney injury, and an early diagnosis of altered kidney function remains a challenge for veterinary practice. The aim of this study was to assess the urine metabolic profile from dogs with babesiosis and different degree of kidney function using untargeted and targeted MS-based metabolomics approaches. In this study, 22 dogs naturally infected with Babesia canis and 12 healthy dogs were included. Untargeted metabolomics approach identified 601 features with a differential abundance between the healthy group and groups of dogs with babesiosis and different level of kidney function, with 27 of them identified as a match to known standards; while targeted approach identified 17 metabolites with significantly different concentrations between the groups. A pattern of significantly altered metabolites referring to the inflammatory host response, oxidative stress, and energy metabolism modulation in babesiosis was presented. Our findings have demonstrated that kidney dysfunction accompanying canine babesiosis was associated with changes in amino acid metabolism, energy metabolism, fatty acid metabolism, and biochemical pathways such as urea cycle and ammonia detoxication. These findings will enable the inclusion of urinary markers for the detection and monitoring of renal damage in babesiosis, as well as in other similar diseases.


2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Andrea García-Quesada ◽  
Ana Jiménez-Rocha ◽  
Juan José Romero-Zuñiga ◽  
Gaby Dolz

AbstractCanine babesiosis is a disease caused by a parasite of the genus Babesia which destroys red blood cells. Previous studies have shown the presence of Babesia vogeli in rural areas in Costa Rica using molecular techniques. The objective of the present study was to determine the seroprevalence and prevalence of B. vogeli in clinically healthy dogs and their ticks at the national level, both within and outside the Central Valley. Blood samples and ticks from 482 dogs were collected between June 2011 and May 2014, and analyzed by immunofluorescence assay (IFA) and real-time polymerase chain reaction (qPCR); two protocols of endpoint PCR and sequencing were used to confirm qPCR-positive samples. Seroprevalence of canine babesiosis of 5.3% (24/453) was determined at the national level, specifically 2.0% (5/253) within and 9.5% (19/200) outside the Central Valley, respectively. Real-time PCR determined a global prevalence of B. vogeli of 31.3% (125/400): 21.4% (47/220) within the Central Valley and 43.3% (78/180) outside the Central Valley. The endpoint PCR amplified only 10 of the 125 blood samples identified as positive in qPCR. One sample amplified by endpoint PCR was sequenced and identified as B. vogeli. Twelve canines were identified with past infections, seven canines with active infection, and 111 canines with early infection. Two species of ticks were found with B. vogeli: Rhipicephalus sanguineus sensu lato (n = 40) and Amblyomma ovale (n = 1). The prevalence of canine babesiosis at the national level, both within and outside the Central Valley, is reported here for the first time, determining the presence of the piroplasmid throughout the country, with a higher circulation of the agent outside the Central Valley. Only one species, B. vogeli, was detected in the blood of dogs and their ticks. Therefore, veterinarians should consider using qPCR to determine the presence of the parasite in blood donors and before starting treatment of vector-borne disease in dogs.


Author(s):  
Samson E. Abalaka ◽  
Joseph O. Omamegbe ◽  
Yila N. Tokara ◽  
Nuhu A. Sani ◽  
Idoko S. Idoko ◽  
...  

Diminizene aceturate (DA) is the drug of choice for treating Canine Trypanosomosis and Canine Babesiosis in many countries of the world. However, co-administration of the drug with long acting Oxytetracycline (OXY-LA) has been associated with nervous signs suggestive of its toxicity, in treated dogs, even at the normal dose. To investigate what causes this toxicity, fourteen Nigerian indigenous dogs were randomly selected into two groups that comprised six dogs each and the remaining untreated two dogs were used for preparation of tissue standards. One group was treated with DA (3.5mg/kg) alone while the other was, additionally, treated with OXY-LA, 10 minutes post treatment (PT) with DA. Two dogs from each group were sacrificed at 240, 360 and 480 hours, PT and their livers, brains, kidneys, hearts and skeletal muscles were harvested and assayed for DA. Mean DA-concentrations in brains of the DA-OXY-LA group (19.71± 1.31a; 15.86± 2.96a; 9.11± 3.31a) were higher (P≤ 0.05) than 1.39 ± 0.45b; 1.05± 0.29b; 0.71 ± 0.30b of the DA-alone group at 240, 360 and 480 hours, PT, respectively. Also, mean-DA concentration in kidneys (8.00 ±0.46a) of the DA-OXY-LA group was significantly (P≤ 0.05) higher than 3.76±0.32b of the DA-alone group at 360 hours PT. These results suggest that OXY-LA enhances DA-accumulation in the brain and reduces its kidney-elimination, thus making the normal dose to act as overdose, which causes the nervous signs often manifested by treated dogs.


2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Wojciech Zygner ◽  
Anna Rodo ◽  
Olga Gójska-Zygner ◽  
Paweł Górski ◽  
Justyna Bartosik ◽  
...  

Abstract Introduction The purpose of the study was to investigate post-mortem changes in dogs infected with Babesia canis and to establish the probable cause of death of the affected animals. Material and Methods Cadavers of six dogs that did not survive babesiosis were collected. Necropsies were performed and samples of various organs were collected for histological examination. Results Necropsies and histological examinations revealed congestion and oedemata in various organs. Most of the dogs had ascites, hydrothorax or hydropericardium, pulmonary oedema, pulmonary, renal, hepatic, and cerebral congestion, and necrosis of cardiomyocytes. Conclusion These results suggested disorders in blood circulation as the most probable cause of death. However, the pulmonary inflammatory response and cerebral babesiosis observed in some of these dogs could also be considered possible causes of death. This study also showed a possible role for renal congestion in the development of renal hypoxia and azotaemia in canine babesiosis.


2021 ◽  
Vol 15 (2) ◽  
pp. 24-28
Author(s):  
O. O. Skornyakova

The purpose of the research is identifying a species of the causative agent of canine babesiosis in the Kirov Region.Materials and methods. To determine the parasite species, we microscopically examined smears from the peripheral blood of sick dogs stained by the Romanowsky-Giemsa method and Leucodif. The microscopy was performed with a binocular microscope Micromed-1. For morphometric assessment of the causative agent, we used a digital camera and software for image analysis ToupView.Results and discussion. We found parasites of various shapes and sizes in erythrocytes of the dogs’ blood in an amount from 1 to 4, rarely 8, 16, and 32. Parasites having large paired pear-shaped forms prevailed (100% in Kirov and 92.86% in Vyatskiye Polyany), which were mainly connected by thin ends at an acute angle and located in the center of an erythrocyte. In Vyatskiye Polyany, we found single small pear-shaped parasites in 7.14%, that were also located along the periphery of an erythrocyte. Based on morphological features, the causative agent of “large” Babesia infection (piroplasmosis) of dogs in the Kirov Region is Babesia (Piroplasma) canis (Piana et Galli Walerio, 1895), and of small Babesia infection of dogs is B. (P.) gibsoni (Patton, 1910). In the Kirov City environment, babesiosis proceeds in monoinfection (81.58% prevalence of infection), and the B. canis infection rate is 28.57% in the south of the Region. We recorded a mixed infection of B. canis + Anaplasma sp. (35.71%), B. canis + Anaplasma sp. + Ehrlichia canis (14.29%), B. canis + E. canis (14.29%), and B. gibsoni + Anaplasma sp. (7.14%) in 71.43%. 


Author(s):  
Majid Khanmohammadi ◽  
Reza Z Olfaghari-Emameh ◽  
Mehdi Arshadi ◽  
Elham Razmjou ◽  
Poorya Karimi

Background: Canine babesiosis is one of the mainly worldwide-distributed tick-borne haemoprotozoan parasitic dis-eases in dogs. Methods: A total of 43 blood samples were randomly collected from naturally infected dogs in seven villages from different geographical areas of Meshkin Shahr, Ardabil Province, Iran. The presence of Babesia species detected with standard methods including parasitological and gene sequencing techniques targeting the 18S rRNA gene. Results: Our results revealed that four dogs 9.3% (4/43) including one female and three male dogs were infected with Babesia. All four Babesia-infected dogs were confirmed B. canis by the molecular-based method. Sequence alignments comparison of the B. canis genotypes A and B, it was revealed that all B. canis isolates belonged to genotype B. Conclusion: This study provides essential data for subsequently define the critical importance of the molecular studies in management and prevention of the canine babesiosis in Iran.  


Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 660
Author(s):  
Stephen J. Goodswen ◽  
Paul J. Kennedy ◽  
John T. Ellis

Babesia infection of red blood cells can cause a severe disease called babesiosis in susceptible hosts. Bovine babesiosis causes global economic loss to the beef and dairy cattle industries, and canine babesiosis is considered a clinically significant disease. Potential therapeutic targets against bovine and canine babesiosis include members of the exportome, i.e., those proteins exported from the parasite into the host red blood cell. We developed three machine learning-derived methods (two novel and one adapted) to predict for every known Babesia bovis, Babesia bigemina, and Babesia canis protein the probability of being an exportome member. Two well-studied apicomplexan-related species, Plasmodium falciparum and Toxoplasma gondii, with extensive experimental evidence on their exportome or excreted/secreted proteins were used as important benchmarks for the three methods. Based on 10-fold cross validation and multiple train–validation–test splits of training data, we expect that over 90% of the predicted probabilities accurately provide a secretory or non-secretory indicator. Only laboratory testing can verify that predicted high exportome membership probabilities are creditable exportome indicators. However, the presented methods at least provide those proteins most worthy of laboratory validation and will ultimately save time and money.


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