Enhanced surfactin fermentation via advanced repeated fed-batch fermentation with increased cell density stimulated by EDTA–Fe (II)

2021 ◽  
Vol 127 ◽  
pp. 288-294
Author(s):  
Yuwei Mei ◽  
Ziyun Yang ◽  
Zhihao Kang ◽  
Fan Yu ◽  
Xuwei Long
Keyword(s):  
Cell Density ◽  
Batch Fermentation ◽  
Fed Batch ◽  
Fed Batch Fermentation

scholarly journals Implementing Chemostat Fermentation of Pichia Pastoris Producing Recombinant HBsAg to Optimize Cell Density Affected by Methanol Rate

2021 ◽  
Vol 11 (5) ◽  
pp. 12633-12641
Keyword(s):  
Pichia Pastoris ◽  
Cell Density ◽  
Batch Fermentation ◽  
Fermentation Process ◽  
Production Efficiency ◽  
Continuous Fermentation ◽  
Scale Up ◽  
Fed Batch ◽  
Simple Method ◽  
Fed Batch Fermentation

High cell density fed-batch fermentation is the main strategy for recombinant hepatitis B surface antigen (rHBsAg) production. In this study, we employed short-term continuous fermentation to optimize the cell density of recombinant Pichia pastoris (P. pastoris). After reaching the maximum specified broth volume of 5 L in the fed-batch fermentation process, the operation mode was altered into the continuous mode with a dilution rate of 0.009 1/h. We used various values of methanol inflow to examine its impact as a limiting nutrient on cell density. After reaching the steady-state point, the continuous fermentation was stopped. The process's performance was evaluated based on titer, yield, productivity, and ease of process control. According to the results, the optimal methanol inflow in the pilot-scale fermentation process was 39.9 ml/h as the cell density increased from 363 g/l wet cell weight (WCW) in the fed-batch stage to 450 g/l WCW. We could successfully scale up the fermentation process with the biomass concentration of 450 g/l without having any major issues such as excessive heat dissipation or insufficient oxygen supply. This approach is a simple method for enhancing rHBsAg production efficiency in P. pastoris without requiring any new and complex facility.

scholarly journals High-Cell-Density Cyclic Fed-Batch Fermentation of a Poly(3-Hydroxybutyrate)-Accumulating Thermophile, Chelatococcus sp. Strain MW10

2010 ◽  
Vol 76 (23) ◽  
pp. 7890-7895 ◽  
Author(s):  
Mohammad H. A. Ibrahim ◽  
Alexander Steinb�chel
Keyword(s):  
Cell Density ◽  
Batch Fermentation ◽  
High Cell Density ◽  
Dry Weight ◽  
Thermophilic Bacterium ◽  
High Cell ◽  
Fed Batch ◽  
G Cell ◽  
Fed Batch Fermentation

ABSTRACT Different fermentation strategies were employed for the cultivation of a new poly(3-hydroxybutyrate)-accumulating thermophilic bacterium, Chelatococcus sp. strain MW10, with the aim of achieving high-cell-density (HCD) growth and high poly(3-hydroxybutyrate) [poly(3HB)] productivity. Enhanced cultivation was achieved by a cyclic fed-batch fermentation (CFBF) technique (42-liter scale). Maximal poly(3HB) productivity was obtained during the second cycle [16.8 � 4.2 g poly(3HB)/liter]. At the end of CFBF (265 h), an HCD of up to 115.0 � 4.3 g cell dry weight/liter was achieved.

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