Protection against free radicals (UVB irradiation) of a water-soluble enzymatic extract from rice bran. Study using human keratinocyte monolayer and reconstructed human epidermis

2010 ◽  
Vol 48 (1) ◽  
pp. 83-88 ◽  
Author(s):  
C. Santa-María ◽  
E. Revilla ◽  
E. Miramontes ◽  
J. Bautista ◽  
A. García-Martínez ◽  
...  
Keyword(s):  
Free Radicals ◽  
Rice Bran ◽  
Water Soluble ◽  
Human Epidermis ◽  
Enzymatic Extract ◽  
Uvb Irradiation ◽  
Reconstructed Human Epidermis ◽  
Human Keratinocyte

Aqueous Enzymatic Method for Preparing the Mixed Extract with Antioxidant Activity from Rice Bran

2013 ◽  
Vol 781-784 ◽  
pp. 792-798 ◽  
Author(s):  
An Min Sun ◽  
Guo Ping Yu ◽  
Yu Xin Wang ◽  
Ji Bian ◽  
Rao Fu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Rice Bran ◽  
Lipase Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Degree Of Hydrolysis ◽  
Enzymatic Extract ◽  
Functional Components

The preparation, approximate composition, degree of hydrolysis (DH), lipase activity and antioxidant capacity (including the ability of scavenging DPPH and ABTS free radicals) were determined for various of aqueous enzymatic extract (AEE) obtained from rice bran (RB) using alcalase (A), trypsin (T), papain (Pn), neutrase (N) and protamex (Px). Peptides and γ-oryzanol are the major functional components of AEE. Generally, the protein yield varied with the type of enzyme used in the order of T>Pn>N>Px>A. Both trypsin and alcalase extracts exhibit a higher level of γ-oryzanol than other enzymes. Lipase activity was measured using the 4-Nitrophenyl Laurate (p-NPL) substrate method, and significantly different (P<0.05) lipase activity values ranging from 0.55 to 2.38 mU/mL were observed from five hydrolysates. Furthermore, two synthetic free radicals were used to evaluate the antioxidant activity of AEE. The alcalase extract (at 4.35±0.15mg protein/mL AEE) shows the greatest scavenging rate (28.42%) of DPPH, but trypsin extract has highest ABTS radical-scavenging activity of 94.05%. Data revealed trypsin was possible the most effective extractant under their optimal conditions. These results demonstrate that the product obtained from rice bran by different enzymatic digestions may contain bioactive components and might potentially be natural antioxidants. Meanwhile, the aqueous enzymatic extraction method can also improve the stability of the hydrolysates.

Prevention of brain protein and lipid oxidation elicited by a water-soluble oryzanol enzymatic extract derived from rice bran

2003 ◽  
Vol 42 (6) ◽  
pp. 307-314 ◽  
Author(s):  
Juan Parrado ◽  
Esther Miramontes ◽  
Mar�a Jover ◽  
Jos� Carlos M�rquez ◽  
Mar�a Angeles Mejias ◽  
...  
Keyword(s):  
Lipid Oxidation ◽  
Rice Bran ◽  
Water Soluble ◽  
Brain Protein ◽  
Enzymatic Extract ◽  
Protein And Lipid Oxidation

Nutraceutical composition, antioxidant activity and hypocholesterolemic effect of a water-soluble enzymatic extract from rice bran

2009 ◽  
Vol 42 (3) ◽  
pp. 387-393 ◽  
Author(s):  
Elisa Revilla ◽  
Consuelo Santa Maria ◽  
E. Miramontes ◽  
Juan Bautista ◽  
Ana García-Martínez ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Rice Bran ◽  
Water Soluble ◽  
Hypocholesterolemic Effect ◽  
Enzymatic Extract

Water-soluble rice bran enzymatic extract attenuates dyslipidemia, hypertension and insulin resistance in obese Zucker rats

2012 ◽  
Vol 52 (2) ◽  
pp. 789-797 ◽  
Author(s):  
Maria L. Justo ◽  
Rosalia Rodriguez–Rodriguez ◽  
Carmen M. Claro ◽  
Maria Alvarez de Sotomayor ◽  
Juan Parrado ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Rice Bran ◽  
Water Soluble ◽  
Zucker Rats ◽  
Enzymatic Extract ◽  
Obese Zucker Rats

Implementation, availability and regulatory status of an OECD accepted Reconstructed Human Epidermis model in Brazil

2018 ◽  
Vol 6 (1) ◽  
pp. 64 ◽  
Author(s):  
Rodrigo De Vecchi ◽  
Vanja Dakic ◽  
Guilherme Mattos ◽  
Anne-Sophie Rigaudeau ◽  
Veronica Oliveira ◽  
...  
Keyword(s):  
Human Epidermis ◽  
Reconstructed Human Epidermis ◽  
Regulatory Status

Malassezia colonisation on a reconstructed human epidermis: Imaging studies

Mycoses ◽  
2019 ◽  
Vol 62 (12) ◽  
pp. 1194-1201 ◽  
Author(s):  
Ana Filipa Pedrosa ◽  
Carmen Lisboa ◽  
Joana Branco ◽  
Ana C. Almeida ◽  
Claudia Mendes ◽  
...  
Keyword(s):  
Human Epidermis ◽  
Imaging Studies ◽  
Reconstructed Human Epidermis
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