Nutraceutical composition, antioxidant activity and hypocholesterolemic effect of a water-soluble enzymatic extract from rice bran

2009 ◽  
Vol 42 (3) ◽  
pp. 387-393 ◽  
Author(s):  
Elisa Revilla ◽  
Consuelo Santa Maria ◽  
E. Miramontes ◽  
Juan Bautista ◽  
Ana García-Martínez ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Rice Bran ◽  
Water Soluble ◽  
Hypocholesterolemic Effect ◽  
Enzymatic Extract

Aqueous Enzymatic Method for Preparing the Mixed Extract with Antioxidant Activity from Rice Bran

2013 ◽  
Vol 781-784 ◽  
pp. 792-798 ◽  
Author(s):  
An Min Sun ◽  
Guo Ping Yu ◽  
Yu Xin Wang ◽  
Ji Bian ◽  
Rao Fu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Rice Bran ◽  
Lipase Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Degree Of Hydrolysis ◽  
Enzymatic Extract ◽  
Functional Components

The preparation, approximate composition, degree of hydrolysis (DH), lipase activity and antioxidant capacity (including the ability of scavenging DPPH and ABTS free radicals) were determined for various of aqueous enzymatic extract (AEE) obtained from rice bran (RB) using alcalase (A), trypsin (T), papain (Pn), neutrase (N) and protamex (Px). Peptides and γ-oryzanol are the major functional components of AEE. Generally, the protein yield varied with the type of enzyme used in the order of T>Pn>N>Px>A. Both trypsin and alcalase extracts exhibit a higher level of γ-oryzanol than other enzymes. Lipase activity was measured using the 4-Nitrophenyl Laurate (p-NPL) substrate method, and significantly different (P<0.05) lipase activity values ranging from 0.55 to 2.38 mU/mL were observed from five hydrolysates. Furthermore, two synthetic free radicals were used to evaluate the antioxidant activity of AEE. The alcalase extract (at 4.35±0.15mg protein/mL AEE) shows the greatest scavenging rate (28.42%) of DPPH, but trypsin extract has highest ABTS radical-scavenging activity of 94.05%. Data revealed trypsin was possible the most effective extractant under their optimal conditions. These results demonstrate that the product obtained from rice bran by different enzymatic digestions may contain bioactive components and might potentially be natural antioxidants. Meanwhile, the aqueous enzymatic extraction method can also improve the stability of the hydrolysates.

Prevention of brain protein and lipid oxidation elicited by a water-soluble oryzanol enzymatic extract derived from rice bran

2003 ◽  
Vol 42 (6) ◽  
pp. 307-314 ◽  
Author(s):  
Juan Parrado ◽  
Esther Miramontes ◽  
Mar�a Jover ◽  
Jos� Carlos M�rquez ◽  
Mar�a Angeles Mejias ◽  
...  
Keyword(s):  
Lipid Oxidation ◽  
Rice Bran ◽  
Water Soluble ◽  
Brain Protein ◽  
Enzymatic Extract ◽  
Protein And Lipid Oxidation

Water-soluble rice bran enzymatic extract attenuates dyslipidemia, hypertension and insulin resistance in obese Zucker rats

2012 ◽  
Vol 52 (2) ◽  
pp. 789-797 ◽  
Author(s):  
Maria L. Justo ◽  
Rosalia Rodriguez–Rodriguez ◽  
Carmen M. Claro ◽  
Maria Alvarez de Sotomayor ◽  
Juan Parrado ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Rice Bran ◽  
Water Soluble ◽  
Zucker Rats ◽  
Enzymatic Extract ◽  
Obese Zucker Rats

Verbascum sinaiticum: A Rich Source of Antioxidant Phenylethanoid Glycosides

2020 ◽  
Vol 10 (2) ◽  
pp. 158-162 ◽  
Author(s):  
Humaira Yasmeen Gondal ◽  
Roshan Zamir ◽  
Muhammad Nisar ◽  
Muhammad Iqbal Choudhary
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Aerial Part ◽  
Methanolic Extract ◽  
Water Soluble ◽  
Antioxidant Potential ◽  
Active Fraction ◽  
Phenylethanoid Glycosides ◽  
Active Constituents ◽  
Soluble Part

Background: The genus Verbascum is well documented for its antioxidant potential but Verbascum sinaiticum is comparatively less studied plant. The current study was carried out to search for antioxidant nutraceuticals from this species. Objective: To explore the antioxidant potential of Verbascum sinaiticum and to identify its active constituents. Methods: The methanolic extract of air-dried aerial part of the Verbascum sinaiticum was partitioned with hexane, chloroform and ethyl acetate. The water-soluble part of ethyl acetate afforded six phenylethanoid glycosides by repeated chromatography over Sephadex LH-20, silica gel and ODS columns. Antioxidant activity of solvent extracts and isolated constituents were evaluated by DPPH, ABTS and FRAP assays. Results: Six phenylethanoid glycosides was isolated and characterized as Verbascoside, Eukovoside, Martynoside, Jionoside D, Campneoside I and Campneoside II, from the most active fraction. Conclusion: Verbascum sinaiticum demonstrated prospective antioxidant activity. The watersoluble part of EtOAc (WSEAE) was found the most active extract whereas Verbascoside was identified as the most potent constituent. All isolated compounds exhibited significant antioxidant activity whereas their synergistic effect was found prominent in the parent fraction.

Antioxidant Capacities of Water-Soluble Substances Extracted from JinHua Ham

2014 ◽  
Vol 675-677 ◽  
pp. 1654-1657
Author(s):  
Xiao Li Zhou ◽  
Xu Chen ◽  
Ting Feng Hao ◽  
Yi Ming Zhou ◽  
Ying Xiao
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Capacity ◽  
Mass Fraction ◽  
Water Soluble ◽  
Oxidation Capacity ◽  
Hydroxyl Free Radical ◽  
Antioxidant Capacities ◽  
Dpph Scavenging ◽  
Jinhua Ham

This study was designed to evaluate the antioxidant activity of samples extracted from Jinhua ham by using such chemical assays as DPPH, scavenging hydroxyl free radical and ABTS. The results demonstrate that antioxidant capacity of the extracted sample is lower than oxidation capacity of Vc. The IC50 spot of samples ranges between 0.6 % and 2.5 % (mass fraction of solute).

New Method To Evaluate Water-Soluble Antioxidant Activity Based on Protein Structural Change

2007 ◽  
Vol 55 (1) ◽  
pp. 165-169 ◽  
Author(s):  
Masaaki Terashima ◽  
Izumi Nakatani ◽  
Ayako Harima ◽  
Sayaka Nakamura ◽  
Masami Shiiba
Keyword(s):  
Antioxidant Activity ◽  
Structural Change ◽  
Water Soluble ◽  
New Method
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