Abstract
This paper explores the relationship among sperm DNA integrity, routine semen parameters and in vitro fertilization (IVF) clinical outcome. It applies sperm chromatin dispersion (SCD) test to conduct sperm DNA fragmentation index (DFI) towards the semen samples of 60 male patients who undergoing assisted reproduction techniques (ART) treatments including intrauterine insemination (IUI), in vitro fertilization (IVF), and intracytoplasmic sperm injection (ICSI) treatment in human assisted reproductive medical technology of our hospital. According to the test results, semen samples are divided into group A (DFI≤10%), group B (10%<DFI<30%) and group C (DFI≥30%) to compare semen routine main parameters in each group as well as fertility rate, cleavage rate and high-quality embryonic rate. There is a negative correlation among DFI and sperm activate rate, progressive motility(PR) rate as well as non-progressive motility(NP) rate. The difference of the sperm activate rate, progressive motility rate and non-progressive motility rate in three groups are statistically significant (P<0.05), and there is no statistically significant difference in sperm concentration (P>0.05). The fertility rate, cleavage rate and high-quality embryonic rate in each group have no statistical significance (P>0.05). Sperm DNA integrity have some connection with routine semen parameters, thus, it could play a guiding role for the analysis of semen routine diagnosis. DFI has little influence on the assisted reproductive technology IVF clinical outcome, which requires follow-up tracking clinical outcome and a large number of samples for validation.
Association Study of Sperm DNA Integrity, Routine Semen Parameters and In Vitro Fertilization Clinical Outcome
