scholarly journals Association Study of Sperm DNA Integrity, Routine Semen Parameters and In Vitro Fertilization Clinical Outcome

2021 ◽  
Author(s):  
Yunzhu Lan ◽  
Xinjian Feng ◽  
Xingyu Sun ◽  
Li Fu
Keyword(s):  
In Vitro Fertilization ◽  
Clinical Outcome ◽  
Semen Parameters ◽  
Dna Integrity ◽  
Cleavage Rate ◽  
Vitro Fertilization ◽  
Progressive Motility ◽  
Sperm Dna Integrity ◽  
Sperm Dna

Abstract This paper explores the relationship among sperm DNA integrity, routine semen parameters and in vitro fertilization (IVF) clinical outcome. It applies sperm chromatin dispersion (SCD) test to conduct sperm DNA fragmentation index (DFI) towards the semen samples of 60 male patients who undergoing assisted reproduction techniques (ART) treatments including intrauterine insemination (IUI), in vitro fertilization (IVF), and intracytoplasmic sperm injection (ICSI) treatment in human assisted reproductive medical technology of our hospital. According to the test results, semen samples are divided into group A (DFI≤10%), group B (10%<DFI<30%) and group C (DFI≥30%) to compare semen routine main parameters in each group as well as fertility rate, cleavage rate and high-quality embryonic rate. There is a negative correlation among DFI and sperm activate rate, progressive motility(PR) rate as well as non-progressive motility(NP) rate. The difference of the sperm activate rate, progressive motility rate and non-progressive motility rate in three groups are statistically significant (P<0.05), and there is no statistically significant difference in sperm concentration (P>0.05). The fertility rate, cleavage rate and high-quality embryonic rate in each group have no statistical significance (P>0.05). Sperm DNA integrity have some connection with routine semen parameters, thus, it could play a guiding role for the analysis of semen routine diagnosis. DFI has little influence on the assisted reproductive technology IVF clinical outcome, which requires follow-up tracking clinical outcome and a large number of samples for validation.

Do sperm DNA integrity tests predict pregnancy with in vitro fertilization?

2008 ◽  
Vol 89 (4) ◽  
pp. 823-831 ◽  
Author(s):  
John A. Collins ◽  
Kurt T. Barnhart ◽  
Peter N. Schlegel
Keyword(s):  
In Vitro Fertilization ◽  
Dna Integrity ◽  
Vitro Fertilization ◽  
Sperm Dna Integrity ◽  
Sperm Dna ◽  
Integrity Tests

The sperm DNA integrity assay (SDI™) a valuable method for assessing sperm vitality in preparation for in vitro fertilization (IVF)

2003 ◽  
Vol 80 ◽  
pp. 113 ◽  
Author(s):  
Peter Ahlering ◽  
Levent Keskintepe ◽  
Geoffrey Sher ◽  
Jeffrey D. Fisch ◽  
Linda Danner ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Dna Integrity ◽  
Vitro Fertilization ◽  
Sperm Dna Integrity ◽  
Sperm Dna ◽  
Valuable Method

Influence of bovine sperm DNA fragmentation and oxidative stress on early embryo in vitro development outcome

Reproduction ◽  
2013 ◽  
Vol 146 (5) ◽  
pp. 433-441 ◽  
Author(s):  
Renata Simões ◽  
Weber Beringui Feitosa ◽  
Adriano Felipe Perez Siqueira ◽  
Marcilio Nichi ◽  
Fabíola Freitas Paula-Lopes ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Fragmentation ◽  
Sperm Chromatin ◽  
Dna Integrity ◽  
Sperm Dna Fragmentation ◽  
Cleavage Rate ◽  
Sperm Dna Integrity ◽  
Sperm Dna ◽  
Significant Difference

Sperm chromatin fragmentation may be caused by a number of factors, the most significant of which is reactive oxygen species. However, little is known about the effect of sperm oxidative stress (OS) on DNA integrity, fertilization, and embryonic development in cattle. Therefore, the goal of this study was to evaluate the influence of sperm OS susceptibility on the DNA fragmentation rate and in vitro embryo production (IVP) in a population of bulls. Groups of cryopreserved sperm samples were divided into four groups, based on their susceptibility to OS (G1, low OS; G2, average OS; G3, high OS; and G4, highest OS). Our results demonstrated that the sperm DNA integrity was compromised in response to increased OS susceptibility. Furthermore, semen samples with lower susceptibility to OS were also less susceptible to DNA damage (G1, 4.06%; G2, 6.09%; G3, 6.19%; and G4, 6.20%). In addition, embryo IVP provided evidence that the embryo cleavage rate decreased as the OS increased (G1, 70.18%; G2, 62.24%; G3, 55.85%; and G4, 50.93%), but no significant difference in the blastocyst rate or the number of blastomeres was observed among the groups. The groups with greater sensitivity to OS were also associated with a greater percentage of apoptotic cells (G1, 2.6%; G2, 2.76%; G3, 5.59%; and G4, 4.49%). In conclusion, we demonstrated that an increased susceptibility to OS compromises sperm DNA integrity and consequently reduces embryo quality.

scholarly journals Sperm DNA Integrity Assessment: A New Tool in Diagnosis and Treatment of Fertility

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Mona Bungum
Keyword(s):  
Semen Quality ◽  
Significant Proportion ◽  
Semen Parameters ◽  
Sperm Chromatin ◽  
Dna Integrity ◽  
Sperm Dna Fragmentation ◽  
Integrity Assessment ◽  
Sperm Dna Integrity ◽  
Sperm Dna

Infertility affects 15% of all couples. Although male infertility factors with reduced semen quality are contributing to about half of all involuntary childlessness, the value of standard semen parameters in prediction of fertilityin vivoand choice of proper method for assisted reproduction is limited. In the search for better markers of male fertility, during the last 10 years, assessment of sperm DNA integrity has emerged as a strong new biomarker of semen quality that may have the potential to discriminate between infertile and fertile men. Sperm DNA Fragmentation Index (DFI) as assessed by the flow cytometric Sperm Chromatin Structure Assay (SCSA) can be used for evaluation of sperm chromatin integrity. The biological background for abnormal DFI is not completely known, but clinical data show that DFI above 30% is associated with very low chance for achieving pregnancy in natural way or by insemination, but notin vitro. Already when the DFI is above 20%, the chance of natural pregnancy may be reduced, despite other sperm parameters being normal. Thus this method may explain a significant proportion of cases of unexplained infertility and can be beneficial in counselling involuntary childless couples need ofin vitrofertilisation.

scholarly journals A Potential Relationship Between Estrogen Receptors Polymorphisms, Sperm Function and in vitro Fertilization Success: A Preliminary Study*

2021 ◽  
Vol 75 ◽  
pp. 304-317
Author(s):  
Joanna Talarczyk-Desole ◽  
Mirosław Andrusiewicz ◽  
Małgorzata Chmielewska ◽  
Anna Berger ◽  
Leszek Pawelczyk ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Estrogen Receptors ◽  
Strong Association ◽  
Fertilization Success ◽  
World Health ◽  
Semen Parameters ◽  
Sperm Function ◽  
Allele Distribution ◽  
Vitro Fertilization

Background: Estrogen receptor 1 (ESR1) and 2 (ESR2) play an important role in regulating fertility in the human reproductive system. Polymorphisms of these receptor genes have been implicated in male infertility in both Chinese and Caucasian populations. However, studies have produced inconsistent results. Spermatozoa defects that result in conception deficiencies could be related to estrogens, their receptors, or genes involved in estrogen-related pathways. This study aims to explore the potential association between the ESR1 and the ESR2 polymorphisms in relation to semen parameters of Caucasian males as well as fertilization success. Materials/Methods: A total of 116 males were included in this study. Forty couples underwent conventional in vitro fertilization, while 76 couples were treated by intracytoplasmic sperm injection. Standard semen analyses were performed according to the World Health Organization criteria. Polymerase chain reaction and restriction fragment length polymorphisms were used to determine genotype and allele distributions. Results: A strong association between the ESR1 rs2234693 recognized by PvuII enzyme, genotype/allele distribution and fertilization success was shown. The T allele occurrence was significantly lower in the case of fertilization failure (p = 0.02). Additionally, the TT genotype was absent in the same group (p=0.02). In the case of the remaining analyzed polymorphisms, little to no interdependence of genotype/allele distribution and fertilization success was noted. Conclusions: Apart from ESR1 rs2234693, the study failed to demonstrate that fertilization success was associated with the selected polymorphisms. In most cases, we did not discover a relationship between both estrogen receptors polymorphisms and sperm function.

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