Randomized double-blind comparison of laser zona pellucida thinning and breaching in frozen–thawed embryo transfer at the cleavage stage

Background: Laser assisted hatching technique based on the hypothesis to make an artificial hole on zona pellucida (ZP) that can help embryo hatching out of ZP easily. This technique has been shown to increase implantation and pregnancy rates in women of advanced age, in women with recurrent implantation failure and following the transfer of frozen–thawed embryos. This study described the outcome of frozen–thawed embryo transfers with laser assisted hatching (LAH), which is one of the safest method in nowadays. Purpose: To assess the effect of assisted hatching technique on the clinical outcomes in vitrified-warmed transfer cycles. Method: A total of 65 thawed-transfer cycles with 153 thawed-embryos undertaken within a 12-month period were analysed, Assisted hatching with laser zona thinning was performed with one-quarter of the zona pellucida circumference. The overall thawed-embryos (day 3) were kept in culture overnight. Patient were prepared the suitable endometrium and transferred embryos advantageously. Results: In which, having the rate of survival embryos were 143 occupying 94.3%, the percentage of grade 1 and 2 embryos occupied 55.9% and 29,3% respectively, and that were enrolled LAH before transfering of frozen–thawed embryos. The average transferred embryos were 2.4±0.8, The rate of implantation per transferred embryos and per transferred embryos cycles was 19.5% and 43.1% respectively. The rate of clinical pregnancies per embryo transfer cycles occupied 33.8% with percentage of early miscarriages (biochemical pregnancies and early clinical miscarriages) was 12.3%. The rate of ongoing pregnancies was 30.8% and multiple pregnancies was low just 12.3%. This result was equal or higher than other researchs in embryos transfer had or no LAH. Conclusion: LAH contributed to stable frozen–thawed embryos transfer effectiveness. Key words: Laser assisted hatching, frozen–thawed embryos transfer, zona pellucida (ZP)

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Does low-dose aspirin improve pregnancy rate in women undergoing frozen-thawed embryo transfer cycle? A pilot double-blind, randomized placebo-controlled trial

Journal of Obstetrics and Gynaecology Research ◽

10.1111/jog.13802 ◽

2018 ◽

Vol 45 (1) ◽

pp. 156-163 ◽

Cited By ~ 2

Author(s):

Tahereh Madani ◽

Firoozeh Ahmadi ◽

Nadia Jahangiri ◽

Akram Bahmanabadi ◽

Narges Bagheri Lankarani

Keyword(s):

Pregnancy Rate ◽

Embryo Transfer ◽

Low Dose ◽

Controlled Trial ◽

Double Blind ◽

Dose Aspirin ◽

Low Dose Aspirin ◽

Frozen Thawed Embryo Transfer ◽

Thawed Embryo Transfer

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Effects of autologous platelet-rich plasma on endometrial expansion in patients undergoing frozen-thawed embryo transfer: A double-blind RCT

International Journal of Reproductive BioMedicine ◽

10.18502/ijrm.v17i6.4816 ◽

2019 ◽

Cited By ~ 3

Author(s):

Leila Nazari ◽

Saghar Salehpour ◽

Sedighe Hoseini ◽

Shahrzad Zadehmodarres ◽

Eznoallah Azargashb

Keyword(s):

Embryo Transfer ◽

Endometrial Thickness ◽

Platelet Rich Plasma ◽

Double Blind ◽

Thin Endometrium ◽

Catheter Group ◽

Autologous Platelet Rich Plasma ◽

Autologous Platelet ◽

Frozen Thawed Embryo Transfer ◽

Thawed Embryo Transfer

Background: Adequate endometrial growth is principal for implantation and pregnancy. Thin endometrium is associated with lower pregnancy rate in assisted reproductive technology. Some frozen-thawed embryo transfer cycles are cancelled due to inadequate endometrial growth. Objective: To assess the effectiveness of autologous platelet-rich plasma (PRP) intrauterine infusion for the treatment of thin endometrium. Materials and Methods: A total of 72 patients who had a history of cancelled frozen-thawed embryo transfer cycle due to the thin endometrium (< 7mm) were assessed for the eligibility to enter the study between 2016 and 2017. Twelve patients were excluded for different reasons, and 60 included patients were randomly assigned to PRP or sham-catheter groups in a double-blind manner. Hormone replacement therapy was administered for endometrial preparation in all participants. PRP intrauterine infusion or sham-catheter was performed on day 11-12 due to the thin endometrium and it was repeated after 48 hr if necessary. Results: Endometrial thickness increased at 48 hr after the first intervention in both groups. All participants needed second intervention due to an inadequate endometrial expansion. After second intervention, endometrial thickness was 7.21 ± 0.18 and 5.76 ± 0.97 mm in the PRP group and sham-catheter group, respectively. There was a significant difference between the two groups. (p < 0.001). Embryo transfer was done for all patients in PRP group and just in six cases in the sham-catheter group. Chemical pregnancy was reported in twelve cases in the PRP group and two cases in the sham-catheter group. Conclusion: According to this trial, PRP was effective in endometrial expansion in patients with refractory thin endometrium.

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