The endometrial receptivity array for diagnosis and personalized embryo transfer as a treatment for patients with repeated implantation failure

2013 ◽  
Vol 100 (3) ◽  
pp. 818-824 ◽  
Author(s):  
Maria Ruiz-Alonso ◽  
David Blesa ◽  
Patricia Díaz-Gimeno ◽  
Eva Gómez ◽  
Manuel Fernández-Sánchez ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Endometrial Receptivity ◽  
Implantation Failure ◽  
Repeated Implantation Failure

scholarly journals The Role of Transcriptomic Biomarkers of Endometrial Receptivity in Personalized Embryo Transfer for Patients with Repeated Implantation Failure

2020 ◽  
Author(s):  
Aihua He ◽  
Yangyun Zou ◽  
Cheng Wan ◽  
Jing Zhao ◽  
Qiong Zhang ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Controlled Trial ◽  
Embryo Implantation ◽  
Endometrial Receptivity ◽  
Training Dataset ◽  
Control Group ◽  
Second Phase ◽  
Implantation Failure ◽  
Repeated Implantation Failure ◽  
Experimental Group

Abstract Background: Window of implantation (WOI) displacement was known as one of endometrial origin leading to embryo implantation failure, especially for repeated implantation failure (RIF). A accurately prediction tool of endometrial receptivity (ER) is extraordinary needed to precisely guide the successful embryo implantation. We aimed to establish an RNA-seq based endometrial receptivity test tool (rsERT) using transcriptomic biomarkers, and to evaluate the benefit of personalized embryo transfer (pET) guided by this tool in patients with repeated implantation failure (RIF).Methods: Two-phase strategy including tool establishment with retrospective data and benefit evaluation with prospective, nonrandomized controlled trial. In the first phase, the rsERT was established by sequencing and analyzing the RNA of endometrial tissues from 50 infertile patients with normal window of implantation (WOI) timing. In the second phase, 142 patients with RIF were recruited and grouped by patient self-selection (experimental group, n=56; control group, n=86). pET guided by rsERT in the experimental group, and conventional ET in the control group. Results: The rsERT, comprising 175 biomarker genes, showed an average accuracy of 98.4% by using 10-fold cross-validation. IPR of experimental group (50.0%) was significantly improved compared to that (23.7%) of control group (RR, 2.107; 95% CI, 1.159 to 3.830; P = 0.017) when transferring day 3 embryos. Although not statistically different, IPR of experimental group (63.6%) was still 20 percentage points higher than that (40.7%) of control group (RR, 1.562; 95% CI, 0.898 to 2.718; P = 0.111) when transferring blastocyst. Regression analysis can precisely predict the optimal WOI time by using all samples as training dataset (R2= 0.92).Conclusions: The rsERT was developed to accurately predict WOI period and significantly improve pregnancy outcomes of patients with RIF, indicating the clinical potential of rsERT-guided pET. Optimization of the model made it possible to predict the optimal WOI by one-point sampling.Trial registration: Chinese Clinical Trial Registry: ChiCTR-DDD-17013375. Registered 14 November 2017, http://www.chictr.org.cn/index.aspx

scholarly journals The role of transcriptomic biomarkers of endometrial receptivity in personalized embryo transfer for patients with repeated implantation failure

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Aihua He ◽  
Yangyun Zou ◽  
Cheng Wan ◽  
Jing Zhao ◽  
Qiong Zhang ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Controlled Trial ◽  
Embryo Implantation ◽  
Endometrial Receptivity ◽  
Control Group ◽  
Second Phase ◽  
Implantation Failure ◽  
Clinical Trial Registry ◽  
Repeated Implantation Failure ◽  
Experimental Group

Abstract Background Window of implantation (WOI) displacement is one of the endometrial origins of embryo implantation failure, especially repeated implantation failure (RIF). An accurate prediction tool for endometrial receptivity (ER) is extraordinarily needed to precisely guide successful embryo implantation. We aimed to establish an RNA-Seq-based endometrial receptivity test (rsERT) tool using transcriptomic biomarkers and to evaluate the benefit of personalized embryo transfer (pET) guided by this tool in patients with RIF. Methods This was a two-phase strategy comprising tool establishment with retrospective data and benefit evaluation with a prospective, nonrandomized controlled trial. In the first phase, rsERT was established by sequencing and analyzing the RNA of endometrial tissues from 50 IVF patients with normal WOI timing. In the second phase, 142 patients with RIF were recruited and grouped by patient self-selection (experimental group, n = 56; control group, n = 86). pET guided by rsERT was performed in the experimental group and conventional ET in the control group. Results The rsERT, comprising 175 biomarker genes, showed an average accuracy of 98.4% by using tenfold cross-validation. The intrauterine pregnancy rate (IPR) of the experimental group (50.0%) was significantly improved compared to that (23.7%) of the control group (RR, 2.107; 95% CI 1.159 to 3.830; P = 0.017) when transferring day-3 embryos. Although not significantly different, the IPR of the experimental group (63.6%) was still 20 percentage points higher than that (40.7%) of the control group (RR, 1.562; 95% CI 0.898 to 2.718; P = 0.111) when transferring blastocysts. Conclusions The rsERT was developed to accurately predict the WOI period and significantly improve the pregnancy outcomes of patients with RIF, indicating the clinical potential of rsERT-guided pET. Trial registration Chinese Clinical Trial Registry: ChiCTR-DDD-17013375. Registered 14 November 2017, http://www.chictr.org.cn/index.aspx

scholarly journals Clinical applications of endometrial receptivity tests in patients with recurrent implantation failure

2021 ◽  
Vol 16 (1) ◽  
pp. 79-85
Author(s):  
Ioan BOLEAC ◽  
◽  
Manuela NEAGU ◽  
Anca CORICOVAC ◽  
Dorina CODREANU ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Embryo Transfer ◽  
Expression Profile ◽  
Clinical Applications ◽  
Endometrial Receptivity ◽  
Clinical Pregnancy ◽  
Implantation Failure ◽  
Recurrent Implantation Failure ◽  
Endometrial Cells

Recurrent implantation failure is represented by the failure to achieve a clinical pregnancy after transfer of at least 4 good-quality embryos in a minimum of 3 fresh or frozen cycles in a woman under the age of 40 years. One of the recent approaches in studying the window of implantation was building the expression profile of the genes of the endometrial cells. We performed a retrospective study which investigated if endometrial receptivity tests improved the outcomes of IVF procedures in patients with recurrent implantation failure. We enrolled 47 couples with RIF and divided them in 2 groups: the first group of 22 couples performed the ERA test and the embryo transfer according to the result of the test; the second group of 27 couples had the embryo transfer done without the ERA test. Our conclusion was that the ERA test did not improve the outcomes for patients with recurrent implantation failure.

Successful pregnancy following double-frozen embryo transfer in a patient with repeated implantation failure

2008 ◽  
Vol 90 (4) ◽  
pp. 1199.e13-1199.e15 ◽  
Author(s):  
Navid Esfandiari ◽  
Joan Coogan-Prewer ◽  
Lynda Gotlieb ◽  
E. Anne Claessens ◽  
Robert F. Casper
Keyword(s):  
Embryo Transfer ◽  
Frozen Embryo Transfer ◽  
Implantation Failure ◽  
Successful Pregnancy ◽  
Repeated Implantation Failure

Up-regulation of miR-145 may contribute to repeated implantation failure after IVF–embryo transfer by targeting PAI-1

2020 ◽  
Vol 40 (5) ◽  
pp. 627-636
Author(s):  
Xuemei Liu ◽  
Huishan Zhao ◽  
Wenshu Li ◽  
Hongchu Bao ◽  
Qinglan Qu ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Implantation Failure ◽  
Repeated Implantation Failure ◽  
Pai 1

scholarly journals Evaluation of Pregnancy Outcomes of Vitrified-Warmed Blastocyst Transfer before and after Endometrial Receptivity Analysis in Identical Patients with Recurrent Implantation Failure

2021 ◽  
pp. 1-7
Author(s):  
Yuta Kasahara ◽  
Tomoko Hashimoto ◽  
Ryo Yokomizo ◽  
Yuya Takeshige ◽  
Koki Yoshinaga ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Pregnancy Outcomes ◽  
Endometrial Receptivity ◽  
Pregnancy Rates ◽  
Blastocyst Transfer ◽  
Implantation Failure ◽  
Recurrent Implantation Failure ◽  
Clinical Value ◽  
Ongoing Pregnancy Rate

Background:The clinical value of personalized embryo transfer (pET) guided by the endometrial receptivity analysis (ERA) tests for recurrent implantation failure (RIF) cases is still unclear. The aim of this study is to clarify the efficacy of ERA leading to personalization of the day of embryo transfer (ET) in RIF patients. Methods: A retrospective study was performed for 94 patients with RIF who underwent ERA between July 2015 and December 2019. Pregnancy outcomes in a previous vitrified-warmed blastocyst transfer (previous VBT) and a personalized vitrified-warmed blastocyst transfer (pVBT) in identical patients were compared. The details of each pVBT were further analyzed between patients in a non-displaced group, which indicated “receptive” cases in ERA results and those who were in the displaced group, which indicated “non-receptive” cases. Results:When the pregnancy rate, both per patient and per transfer cycle, of previous VBT and pVBT were compared, a significant increase in pVBT was observed between the two methods (5.3% vs. 62.8%, 4.4% vs. 47.9%, respectively). The pregnancy rates, implantation rates, and clinical pregnancy rates of the first pVBT were significantly higher in the displaced group than the non-displaced group. The cumulative ongoing pregnancy rate of the displaced group tended to be higher compared to that of the non-displaced group in the first pVBT, although the difference was not statistically significant (51.0% vs. 31.1%, [Formula: see text] = 0.06). Conclusions:Our study demonstrates that pVBT guided by ERA tests may improve pregnancy outcomes in RIF patients whose window of implantation (WOI) is displaced, and its effect may be more pronounced at the first pVBT. The displacement of WOI may be considered to be one of the causes of RIF, and its adjustment may contribute to the improvement of pregnancy outcomes in RIF patients.

Modern methods of influence at endometrial receptivity in patients with recurrent implantation failure (review)

GYNECOLOGY ◽  
2018 ◽  
Vol 20 (3) ◽  
pp. 66-70
Author(s):  
M I Polovneva ◽  
I E Korneeva ◽  
O V Bourmenskaya
Keyword(s):  
Mononuclear Cells ◽  
Platelet Rich Plasma ◽  
Test System ◽  
Endometrial Receptivity ◽  
Implantation Failure ◽  
Recurrent Implantation Failure ◽  
Peripheral Blood Mononuclear ◽  
Repeated Implantation Failure ◽  
Endometrial Scratching ◽  
Modern Methods

Objective. To carry out an analysis of the data available in scientific literature on modern methods of influence at endometrial receptivity in patients with recurrent implantation failure. Materials and methods. The review includes the data of foreign and Russia papers published on PubMed during the last 7-10 years. Results. There are studies described the role of endometrial scratching, infusion granulocyte colony stimulating factor, autologous peripheral blood mononuclear cells, autologous platelet-rich plasma, the endometrial receptivity array in treatment for patients with repeated implantation failure. Conclusion. Several adjuvant therapies and diagnostic tests have been used along with IVF to increase the pregnancy rates for women with repeated implantation failure. Perhaps a new test-system to find personal predictors of endometrial receptivity can tern up a positive effect at patients with RIF.

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