Follow-up of child growth regarding new technologies: testicular sperm extraction (TESE), in vitro maturation (IVM), and assisted oocyte activation (AOA)

2015 ◽  
Vol 104 (3) ◽  
pp. e290
Author(s):  
N. Matsukawa ◽  
S. Shibasaki ◽  
M. Takahashi ◽  
C. Sasaki ◽  
Y. Nakamura ◽  
...  
Keyword(s):  
In Vitro Maturation ◽  
New Technologies ◽  
Child Growth ◽  
Testicular Sperm Extraction ◽  
Oocyte Activation ◽  
Testicular Sperm ◽  
Assisted Oocyte Activation

scholarly journals Obstetrical and Neonatal Outcomes of 3,028 Singletons Born After Advanced ART Techniques: Ejaculated Sperm Intracytoplasmic Sperm Injection, Artificial Oocyte Activation, in Vitro Maturation and Testicular Sperm Extraction

2021 ◽  
pp. 1-10
Author(s):  
Hiromitsu Hattori ◽  
Yuko Atsumi ◽  
Yukiko Nakajo ◽  
Nobuya Aono ◽  
Masae Koizumi ◽  
...  
Keyword(s):  
Sex Ratio ◽  
Intracytoplasmic Sperm Injection ◽  
In Vitro Maturation ◽  
The Other ◽  
Neonatal Outcomes ◽  
Testicular Sperm Extraction ◽  
Oocyte Activation ◽  
Testicular Sperm ◽  
Art Techniques

Background: To evaluate obstetrical and neonatal outcomes of singletons conceived after advanced assisted reproductive technology (ART) techniques: conventional IVF pregnancies (C-IVF), ejaculated sperm intracytoplasmic sperm injection (ICSI), assisted oocyte activation (AOA), in vitro maturation (IVM), and testicular sperm extraction (TESE). Methods:The subjects were 3,028 singletons who were born after fresh or frozen embryo transfer. The subjects were separated into five groups: C-IVF (n = 855), ICSI (n = 1,869), AOA (n = 42), IVM (n = 32), and TESE (n = 230). We evaluated obstetrical and neonatal outcomes calculating the adjusted odds ratio (AOR) using multivariable logistic regression analyses for fresh and frozen embryos and for cleavage and blastocyst transfer. The C-IVF group was used as a background control for the ICSI group. Moreover, the TESE, AOA, and IVM groups were compared to the ICSI group to evaluate the effects of the ICSI procedure itself. Results: The incidence of perinatal complications was significantly lower in the ICSI-fresh group (AOR = 0.29, 95% CI: 0.10–0.83, p < 0.05). Regarding sex ratio, the IVM was significantly associated with sex ratio imbalance toward female in both fresh and frozen groups (AOR = 0.30, 95% CI: 0.10–0.96, AOR = 0.27, 95% CI: 0.07–0.98, p < 0.05). On the other hand, there were no significant differences in preterm birth rate, low birth weight rate and congenital abnormalities rate between conventional IVF, ICSI, and the other groups. Conclusions: There were no negative effects on obstetrical and neonatal outcomes between conventional IVF and ICSI. Although this was a limited sample size study, advanced ART technologies such as AOA, IVM, and TESE also seem to have a low risk of adverse impact on obstetrical and neonatal outcomes but may have a slight impact on sex ratio.

Outcome of cycles of oocyte in vitro maturation requiring testicular sperm extraction for nonobstructive azoospermia

2011 ◽  
Vol 96 (2) ◽  
pp. 321-323 ◽  
Author(s):  
Rubens Fadini ◽  
Elisabetta Colpi ◽  
Mario Mignini Renzini ◽  
Giovanni Coticchio ◽  
Ruggero Comi ◽  
...  
Keyword(s):  
In Vitro Maturation ◽  
Testicular Sperm Extraction ◽  
Nonobstructive Azoospermia ◽  
Testicular Sperm

334 EFFECT OF INSULIN ON IN VITRO MATURATION OF CANINE OOCYTES

2006 ◽  
Vol 18 (2) ◽  
pp. 275
Author(s):  
H. S. Lee ◽  
Y. I. Seo ◽  
X. J. Yin ◽  
S. G. Cho ◽  
I. H. Bae ◽  
...  
Keyword(s):  
In Vitro Maturation ◽  
Uv Light ◽  
Cumulus Cells ◽  
Oocyte Activation ◽  
Cell Stage ◽  
Oocyte Competence ◽  
Treatment Groups ◽  
Maturation Medium ◽  
Oviduct Fluid

In spite of our increased knowledge of in vitro oocyte maturation techniques, the success rate of obtaining mature canine oocytes in vitro remains very low compared with that for other domestic animals. The inefficient rate of meiotic resumption of canine oocytes is probably due to both the unique reproductive cycle and inappropriate in vitro maturation (IVM) medium. In an unpublished experiment, we found that the concentration of insulin was higher in estrus bitch serum (EBS; 8833 pg/mL) than in dog follicular fluid (DFF; preovulatory follicle, 122 pg/mL), which implies its possible role in the acquisition of oocyte competence. Therefore, in the present study we investigated the effects of supplementing the IVM medium with insulin on the incidence of maturation to metaphase II. Ovaries were collected from various stages of the estrous cycle by ovariohysterectomy, and oocytes with two or more intact cumulus layers and with a diameter >110 �m were selected and used for IVM. Oocytes were cultured in modified synthetic oviduct fluid (2004 Reprod. Nutr. Dev. 44, 105-109) supplemented with 10% EBS, 20 �g/mL estradiol, and different concentrations of insulin (0, 10, 100, or 1000 ng/mL) at 38.5�C, 5% CO2 in air. After 72 h, cumulus cells were removed from around oocytes using a small glass pipette. Denuded oocytes were fixed in 3.7% paraformaldehyde supplemented with 10 �g/mL Hoechst 33342 at room temperature for 40 min. Nuclear status was observed under UV light using a fluorescence microscope. The percentage of oocytes at the metaphase II stage was not different among the four groups 6.8, 1.8, 5.4, and 2.1% in the control, 10, 100, and 1000 ng/mL insulin groups, respectively. The incidence of oocytes with pronuclear-like structures or cleaving beyond the two-cell stage was not significant higher in the 10 and 100 ng/mL insulin treatment groups than in the control and 1000 ng/mL insulin groups 20.0 and 19.6% vs. 6.8 and 6.4%, respectively. These results indicate that the addition of insulin to the in vitro maturation medium of dog oocytes had no effect on the incidence of meiotic maturation to metaphase II, nor did it affect the frequency of occurrence of spontaneous oocyte activation.

scholarly journals O-119. Sonographic and Doppler flow follow-up after testicular sperm extraction and percutaneous sperm aspiration

1997 ◽  
Vol 12 (Suppl_2) ◽  
pp. 57-58 ◽  
Author(s):  
R. Ron-El ◽  
S. Strauss ◽  
S. Friedler ◽  
D. Strassburger ◽  
D. Komarovsky ◽  
...  
Keyword(s):  
Testicular Sperm Extraction ◽  
Testicular Sperm ◽  
Doppler Flow ◽  
Sperm Aspiration
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