The selective anti-fungal metabolites from Irpex lacteus and applications in the chemical interaction of Gastrodia elata, Armillaria sp., and endophytes

Pseudomonas fluorescens not only enhances the plant growth but also controls the fungal pathogens by production of anti fungal metabolites. The objective of this experiment was to identify P. fluorescens using different biochemical tests. This research was carried out in Plant Pathology Laboratory at Agriculture and Forestry University (AFU), Rampur, Chitwan, Nepal. The result of this experiment indicated that P. fluorescens gave positive result for Catalase test, Gelatin liquefaction, Fluorescent pigment and Oxidase test but negative result for starch hydrolysis test. The colony of P. fluorescens was maximum in maize seed than that of rice seed after two hours of inoculation, whereas higher number of colony was found in rice seed than that of maize seed after twenty four hours of seed inoculation. This findings is useful for identifying colony of P. fluorescens per seed which is necessary for better seedling growth and effective biological control of pathogens.

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Purification and characterization of a novel anti-fungal protein from Gastrodia elata

Plant Physiology and Biochemistry ◽

10.1016/s0981-9428(99)80008-4 ◽

1998 ◽

Vol 36 (12) ◽

pp. 899-905 ◽

Cited By ~ 33

Author(s):

Qing Xu ◽

Ying Liu ◽

Xiaochen Wang ◽

Hongya Gu ◽

Zhangliang Chen

Keyword(s):

Purification And Characterization ◽

Gastrodia Elata ◽

Fungal Protein ◽

Anti Fungal

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Editorial (Thematic Issue: Medicinal Chemistry from Fungi and Back: Discovery of Novel Anti-Fungal Drugs and Mycotherapy of Cancer and Other Diseases with Fungal Metabolites)

Current Topics in Medicinal Chemistry ◽

10.2174/15680266113136660190 ◽

2013 ◽

Vol 13 (21) ◽

pp. 2647-2647

Author(s):

Dejan Stojkovic ◽

Isabel Ferreria ◽

Marina Sokovic

Keyword(s):

Medicinal Chemistry ◽

Thematic Issue ◽

Fungal Metabolites ◽

Anti Fungal

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Cupressus macrocarpa Heartwood Oil and its Bioactivity against some Wood Decay Fungi

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.485.413 ◽

2012 ◽

Vol 485 ◽

pp. 413-416 ◽

Cited By ~ 2

Author(s):

Li Jun Zhang ◽

Yuan Yuan Zhang ◽

Shu Jun Li ◽

J.J. Karchesy

Keyword(s):

Steam Distillation ◽

Wood Decay ◽

Irpex Lacteus ◽

Decay Fungi ◽

Filter Paper Disc ◽

Postia Placenta ◽

Fungal Activity ◽

Wood Decay Fungi ◽

Anti Fungal ◽

Paper Disc

Cupressus macrocarpa (Monterey cypress) heartwood has natural durability. The heartwood oil was prepared by steam distillation and its anti-fungal activity was tested against four wood decay fungi, i.e. Trametes versicolor, Irpex lacteus, Gloeophyllum trabeum, and Postia placenta with a filter paper disc method. The oil was active against all these fungi at the concentration of 8 mg.mL-1 or greater, but Postia placenta was the most difficult for the oil to inhibit. GC-MS was adopted to analyze the components of the heartwood oil. Two components, 4-terpineol and carvacrol were identified and carvacrol represented 94.428% of the oil. The oil had anti-fungal activity mostly because of carvacrol in the high content

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Anti-Fungal Activity Characterization of Four Rosinyl Quaternary Ammonium Salts

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.183-185.810 ◽

2011 ◽

Vol 183-185 ◽

pp. 810-813

Author(s):

Tao Liang ◽

Jian Li ◽

Shu Jun Li ◽

Yan Jin

Keyword(s):

Quaternary Ammonium ◽

Trametes Versicolor ◽

Quaternary Ammonium Salts ◽

Ammonium Salts ◽

White Rot ◽

Chaetomium Globosum ◽

White Rot Fungus ◽

Gloeophyllum Trabeum ◽

Irpex Lacteus ◽

Anti Fungal

The anti-fungal activities of four self-prepared rosinyl quaternary ammonium salts (QAS), QASA, BQAS1, BQAS2 and DQAS, were evaluated with paper disc method in this paper. The result showed that, all the QAS of rosin were bioactive to the selected fungi, Irpex lacteus, Trametes versicolor, Phanerochaete chrysosporium, Gloeophyllum trabeum, Chaetomium globosum, and Paecilomyces variotii. There were obvious inhibition halos for all QAS to the fungi. The result proved that the QAS of rosin were broad-spectrum anti-fungal. Especially, to white rot fungus Trametes versicolor, brown rot fungus Gloeophyllum trabeum and soft rot fungus Chaetomium globosum, they were more effective. When the concentration reached 3.2% (w/w), the inhibition halo diameters all met or exceeded 25mm. Among these four anti-fungal derivatives of rosin, the Gemini QAS (BQAS1 and BQAS2) had bigger inhibition halo diameters than the others, which indicated that the Gemini QAS of rosin were more anti-fungal than the normal QAS of rosin.

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Synthesis of a Bi-Quaternary Ammonium Salt from Rosin and its Inhibition to some Wood Decay Fungi

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.174-177.478 ◽

2012 ◽

Vol 174-177 ◽

pp. 478-482 ◽

Cited By ~ 1

Author(s):

Thi Thanh Hien Nguyen ◽

Shu Jun Li ◽

Jian Li ◽

Yan Jin ◽

Nan Xue Liu

Keyword(s):

Ammonium Chloride ◽

Ammonium Salt ◽

Quaternary Ammonium ◽

Quaternary Ammonium Salt ◽

Inhibitory Effect ◽

Gravimetric Analysis ◽

Irpex Lacteus ◽

Decay Fungi ◽

Anti Fungal

An anti-fungal derivative of rosin, bi-quaternary ammonium salt (B-QAS) was synthesized. First, rosin was modified by acrylic acid. Then using this modified rosin reacted with an intermediate quaternary ammonium (epoxy propyl triethyl ammonium chloride) which was the reaction product of triethylamine and epichlorohydrin. The final product was bisN-(3-rosin acyloxy -2-hydroxy) propyl-N, N triethyl ammonium chloride. The chemical structure of the product and its functional groups was characterized by Fourier transform infrared spectroscopy (FTIR). Its yield of greater than 80% was conformed by high performance liquid chromatography (HPLC) and gravimetric analysis. Its anti-fungal activity was determined in vitro with a paper-disc method. B-QAS presented significant inhibitory effect for Trametes versicolor, Gloeophyllum trabeum and Postia placenta when its concentration was 0.5 mg/mL, whereas for Irpex lacteus the inhibitory effect was a little poor.

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GRAPHENE CONJUGATED USNIC ACID NANO-FORMULATION FOR THE TREATMENT OF TOPICAL FUNGAL INFECTION

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2020v12i5.34724 ◽

2020 ◽

pp. 47-53

Author(s):

NIMESH KUMAR DUBEY ◽

SHANTI BHUSHAN MISHRA ◽

ALOK MUKERJEE ◽

AKANKSHA SINGH

Keyword(s):

Statistical Analysis ◽

Drug Release ◽

Fungal Infection ◽

Chemical Interaction ◽

Usnic Acid ◽

Precipitation Method ◽

Albino Rats ◽

Fungal Activity ◽

Anti Fungal ◽

Nano Formulation

Objective: The study aims to investigate the antifungal response of the dug usnic acid with the carrier graphene. Methods: Nano-precipitation method by sonication was adopted to formulate the conjugate. SEM test was performed to check the shape and average size of the conjugate. FTIR test was performed for the chemical interaction between the drug and the carrier. Ointment was prepared by the fusion method and the viscosity test was performed by Brookfield viscometer. Spreadability test was performed by slide method. Animal activity was performed to confirm the antifungal effect of the formulated nano-conjugate. Statistical analysis was done by Anova. Results: SEM study shows that the conjugate is in the nano range and possess a spherical shape. FTIR study shows no interaction between the drug and the carrier. The result of in vitro drug release study shows that the conjugate posses a higher drug release rate as compared to the drug alone. Topical drug administration is more suitable for the treatment of the fungal infection, so the nano-conjugate was incorporated into the ointment by geometric mixing. The viscosity and the spreadability test were performed on the different formulations of the ointment and the suitable one was selected for the topical administration. Anti-fungal study had been performed on the Wistar albino rats for 6 d. Skin culture of rats was performed for the formation of the fungal colonies. Statistical analysis by Anova gives p<0.001. It was found that the normal form of usnic acid, graphene and the nano form both possess anti-fungal activity as 3/6 and 2/6 experimental animals are cured by normal formulation and nano-formulation. Conclusion: The present anti-fungal study revealed that the nano-form of the conjugate possess higher anti-fungal activity than the normal formulation of usnic acid with graphene.

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