Predilection sites for Toxoplasma gondii in sheep tissues revealed by magnetic capture and real-time PCR detection

2015 ◽  
Vol 52 ◽  
pp. 150-153 ◽  
Author(s):  
Jana Juránková ◽  
Walter Basso ◽  
Helena Neumayerová ◽  
Anita Frencová ◽  
Vojtech Baláž ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Detection ◽  
Magnetic Capture

A more sensitive, efficient and ISO 17025 validated Magnetic Capture real time PCR method for the detection of archetypal Toxoplasma gondii strains in meat

2017 ◽  
Vol 47 (13) ◽  
pp. 875-884 ◽  
Author(s):  
Ignacio Gisbert Algaba ◽  
Manon Geerts ◽  
Malgorzata Jennes ◽  
Wim Coucke ◽  
Marieke Opsteegh ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Real Time ◽  
Real Time Pcr ◽  
Iso 17025 ◽  
Magnetic Capture ◽  
Pcr Method

Brain is the predilection site of Toxoplasma gondii in experimentally inoculated pigs as revealed by magnetic capture and real-time PCR

2014 ◽  
Vol 38 ◽  
pp. 167-170 ◽  
Author(s):  
Jana Juránková ◽  
Walter Basso ◽  
Helena Neumayerová ◽  
Vojtech Baláž ◽  
Eva Jánová ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Real Time ◽  
Real Time Pcr ◽  
Magnetic Capture ◽  
Predilection Site

Real-time PCR detection of Toxoplasma gondii in surface water samples in São Paulo, Brazil

2019 ◽  
Vol 118 (2) ◽  
pp. 631-640 ◽  
Author(s):  
Ana Tereza Galvani ◽  
Ana Paula Guarnieri Christ ◽  
José Antonio Padula ◽  
Mikaela Renata Funada Barbosa ◽  
Ronalda Silva de Araújo ◽  
...  
Keyword(s):  
Surface Water ◽  
Toxoplasma Gondii ◽  
Real Time ◽  
Water Samples ◽  
Real Time Pcr ◽  
Sao Paulo ◽  
Pcr Detection ◽  
São Paulo ◽  
Surface Water Samples

Comparison of real-time PCR detection methods for B1 and P30 genes of Toxoplasma gondii

2003 ◽  
Vol 45 (4) ◽  
pp. 269-271 ◽  
Author(s):  
Susanne Buchbinder ◽  
Rosemarie Blatz ◽  
Arne Christian Rodloff
Keyword(s):  
Toxoplasma Gondii ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Detection ◽  
Detection Methods

Quantification of Toxoplasma gondii in tissue samples of experimentally infected goats by magnetic capture and real-time PCR

2013 ◽  
Vol 193 (1-3) ◽  
pp. 95-99 ◽  
Author(s):  
Jana Juránková ◽  
Marieke Opsteegh ◽  
Helena Neumayerová ◽  
Kamil Kovařčík ◽  
Anita Frencová ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Real Time ◽  
Real Time Pcr ◽  
Tissue Samples ◽  
Magnetic Capture

scholarly journals Persistence of SARS-CoV-2 Viral RNA in Nasopharyngeal Swabs after Death: An Observational Study

2021 ◽  
Vol 9 (4) ◽  
pp. 800
Author(s):  
Francesca Servadei ◽  
Silvestro Mauriello ◽  
Manuel Scimeca ◽  
Bartolo Caggiano ◽  
Marco Ciotti ◽  
...  
Keyword(s):  
Viral Load ◽  
Observational Study ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Detection ◽  
Viral Rna ◽  
Clinical Documentation ◽  
Post Mortem ◽  
Medical Assistance ◽  
Time Points

The aim of this study was to investigate the persistence of SARS-CoV-2 in post-mortem swabs of subjects who died from SARS-CoV-2 infection. The presence of the virus was evaluated post-mortem from airways of 27 SARS-CoV-2 positive patients at three different time points (T1 2 h; T2 12 h; T3 24 h) by real-time PCR. Detection of antibodies to SARS-CoV-2 was performed by Maglumi 2019-nCoV IgM/IgG chemiluminescence assay. SARS-CoV-2 viral RNA was still detectable in 70.3% of cases within 2 h after death and in 66,6% of cases up to 24 h after death. Our data showed an increase of the viral load in 78,6% of positive individuals 24 h post-mortem (T3) in comparison to that evaluated 2 h after death (T1). Noteworthy, we detected a positive T3 post-mortem swab (24 h after death) from 4 subjects who were negative at T1 (2 h after death). The results of our study may have an important value in the management of deceased subjects not only with a suspected or confirmed diagnosis of SARS-CoV-2, but also for unspecified causes and in the absence of clinical documentation or medical assistance.

scholarly journals Satellite DNA as a target for TaqMan real-time PCR detection of the pinewood nematode, Bursaphelenchus xylophilus

2007 ◽  
Vol 8 (6) ◽  
pp. 803-809 ◽  
Author(s):  
CECILE FRANÇOIS ◽  
CHANTAL CASTAGNONE ◽  
NEIL BOONHAM ◽  
JENNY TOMLINSON ◽  
REBECCA LAWSON ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Satellite Dna ◽  
Bursaphelenchus Xylophilus ◽  
Pcr Detection ◽  
Pinewood Nematode
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