Kosakonia radicincitans and Cryptococcus laurentii controlled Penicillium expansum rot and decreased patulin production at 4 and 25 °C

2021 ◽  
pp. 103863
Author(s):  
Yesica Lambrese ◽  
Gabriela Sansone ◽  
María Isabel Sanz ◽  
Susana Noemí Di Masi ◽  
Julio Raba ◽  
...  
Keyword(s):  
Penicillium Expansum ◽  
Cryptococcus Laurentii

scholarly journals Indole-3-Acetic Acid Improves Postharvest Biological Control of Blue Mold Rot of Apple by Cryptococcus laurentii

2009 ◽  
Vol 99 (3) ◽  
pp. 258-264 ◽  
Author(s):  
Ting Yu ◽  
Jishuang Chen ◽  
Huangping Lu ◽  
Xiaodong Zheng
Keyword(s):  
Acetic Acid ◽  
Apple Fruit ◽  
Natural Resistance ◽  
Penicillium Expansum ◽  
Cryptococcus Laurentii ◽  
Blue Mold ◽  
Biocontrol Efficacy ◽  
Indole 3 Acetic Acid

Cryptococcus laurentii is a well-known postharvest biocontrol yeast; however, it cannot provide satisfactory levels of decay control when used alone. Here, we evaluated the effects of indole-3-acetic acid (IAA), a plant growth regulator, on the biocontrol efficacy of the yeast antagonist C. laurentii against blue mold rot caused by Penicillium expansum in apple fruit. Results showed that the addition of IAA at 20 μg/ml to suspensions of C. laurentii greatly enhanced inhibition of mold rot in apple wounds compared with that observed with C. laurentii alone. The addition of IAA at 20 μg/ml or lower did not influence the population growth of C. laurentii in wounds, but adverse effects were seen on C. laurentii when the concentration of IAA was increased to 200 μg/ml or above in vitro and in vivo. P. expansum infection in apple wounds was not inhibited when the pathogen was inoculated into the fruit wounds within 2 h after application of IAA; however, infection was reduced when inoculated more than 12 h after IAA application. Treatment of wounds with IAA at 20 μg/ml 24 h before pathogen inoculation resulted in significant inhibition of P. expansum spore germination and host infection. Application of IAA at 20 μg/ml also reduced P. expansum infection when it was applied 48 h before pathogen inoculation in the intact fruit. Thus, IAA could reinforce the biocontrol efficacy of C. laurentii in inhibiting blue mold of apple fruit by induction of the natural resistance of the fruit.

scholarly journals Control of Postharvest Decay in Pear by Four Laboratory-Grown Yeasts and Two Registered Biocontrol Products

Plant Disease ◽  
1999 ◽  
Vol 83 (2) ◽  
pp. 155-158 ◽  
Author(s):  
David Sugar ◽  
Robert A. Spotts
Keyword(s):  
Pseudomonas Syringae ◽  
Rhodotorula Glutinis ◽  
Penicillium Expansum ◽  
First Year ◽  
Cryptococcus Laurentii ◽  
Blue Mold ◽  
Lesion Diameter ◽  
Candida Oleophila ◽  
Resistant Strains ◽  
Second Year

Control of blue mold decay in Bosc pears was studied with the laboratory-grown yeasts Rhodotorula glutinis, Cryptococcus infirmo-miniatus, and two strains of Cryptococcus laurentii, as well as registered biocontrol products Aspire, containing the yeast Candida oleophila, and Bio-Save 11 (now Bio-Save 110), containing the bacterium Pseudomonas syringae. Both thiabendazole (TBZ)-sensitive and TBZ-resistant strains of Penicillium expansum were used. Aspire treatment reduced the average lesion diameter by approximately 65 and 45%, and reduced decay incidence by 27 and 9% with TBZ-resistant and TBZ-sensitive P. expansum, respectively, in the first year of the study, but did not result in significant decay control in the second year. Bio-Save 11 reduced decay lesion diameter by 32 to 72% and incidence by 21 to 40% over the 2 years. In both years, TBZ-sensitive P. expansum was completely controlled by the combination of either C. laurentii (both strains), R. glutinis, or C. infirmo-miniatus with 100 ppm TBZ. With TBZ-resistant P. expansum, control of wound infection with these yeasts alone or with 100 ppm TBZ ranged from 62.9 to 100%. In a packinghouse trial, control by Bio-Save 110 + 100 ppm TBZ and Aspire + 100 ppm TBZ was not different than control by TBZ at 569 ppm, the maximum label rate. The amount of decay following Aspire + 100 ppm TBZ treatment was significantly less than the amount of decay following Bio-Save 110 + 100 ppm TBZ treatment.

Inhibiting Penicillium expansum infection on pear fruit by Cryptococcus laurentii and cytokinin

2007 ◽  
Vol 45 (2) ◽  
pp. 221-227 ◽  
Author(s):  
Xiaodong Zheng ◽  
Ting Yu ◽  
Rongle Chen ◽  
Bin Huang ◽  
Vivian Chi-Hua Wu
Keyword(s):  
Penicillium Expansum ◽  
Cryptococcus Laurentii ◽  
Pear Fruit

Effect of Cryptococcus laurentii and calcium chloride on control of Penicillium expansum and Botrytis cinerea infections in pear fruit

2012 ◽  
Vol 61 (2) ◽  
pp. 169-175 ◽  
Author(s):  
Ting Yu ◽  
Chen Yu ◽  
Huangping Lu ◽  
Mahbuba Zunun ◽  
Fangxia Chen ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Calcium Chloride ◽  
Penicillium Expansum ◽  
Cryptococcus Laurentii ◽  
Pear Fruit

scholarly journals Effect of the Biocontrol Yeast Rhodotorula glutinis Strain LS11 on Patulin Accumulation in Stored Apples

2005 ◽  
Vol 95 (11) ◽  
pp. 1271-1278 ◽  
Author(s):  
Raffaello Castoria ◽  
Valeria Morena ◽  
Leonardo Caputo ◽  
Gianfranco Panfili ◽  
Filippo De Curtis ◽  
...  
Keyword(s):  
Rhodotorula Glutinis ◽  
Biocontrol Agents ◽  
Yeast Cells ◽  
Penicillium Expansum ◽  
Cryptococcus Laurentii ◽  
Fungicide Residues ◽  
Tlc Plates ◽  
Layer Chromatography

Contamination of apples (Malus domestica) and derived juices with fungicide residues and patulin produced by Penicillium expansum are major issues of food safety. Biocontrol agents represent an alternative or supplement to chemicals for disease control. Our data show that these microbes could also contribute to actively decreasing patulin accumulation in apples. Three biocontrol agents, Rhodotorula glutinis LS11, Cryptococcus laurentii LS28, and Aureobasidium pullulans LS30, were examined for their in vitro growth in the presence of patulin and for their capability to decrease mycotoxin recovery from the medium. Strain LS11 yielded the highest growth rates and the greatest decrease of toxin recoveries. Further, it caused the appearance of two major spots on thin-layer chromatography (TLC) plates, suggesting possible metabolization of the mycotoxin. In vivo, i.e., in the low percentage of LS11-pretreated apples infected by P. expansum, patulin accumulation was significantly lower than in nontreated infected fruits. Yeast cells survived and increased in infected apples and, in a model system emulating decaying apple, resulted in accelerated breakdown of patulin and the production of the same TLC spots as those detected in vitro. These data suggest that biocontrol yeast cells surviving in decaying apples could metabolize patulin and/or negatively affect its accumulation or synthesis. To our knowledge, this is the first report describing the effect of a biocontrol agent on patulin accumulation in vivo.

Integration of Biocontrol Agents and Food-Grade Additives for Enhancing Protection of Stored Apples from Penicillium expansum

2005 ◽  
Vol 68 (10) ◽  
pp. 2100-2106 ◽  
Author(s):  
G. LIMA ◽  
A. M. SPINA ◽  
R. CASTORIA ◽  
F. DE CURTIS ◽  
V. DE CICCO
Keyword(s):  
Aureobasidium Pullulans ◽  
Synergistic Effects ◽  
Rhodotorula Glutinis ◽  
Antagonistic Activity ◽  
Biocontrol Agents ◽  
Penicillium Expansum ◽  
Cryptococcus Laurentii ◽  
Blue Mold ◽  
Food Grade ◽  
Yeast Concentration

Forty-nine compounds currently used as additives in foods were tested in combination with three biocontrol agents, the yeasts Rhodotorula glutinis, Cryptococcus laurentii, and the yeastlike fungus Aureobasidium pullulans, to increase their antagonistic activity against Penicillium expansum, the causal agent of blue mold on apples. Twelve additives dramatically improved the antagonistic activity of one or more of the tested biocontrol agents. In a two-way factorial experiment with these selected additives the percentage of P. expansum rots on apples was significantly influenced by the antagonist and the additive as well as by their interaction. The combination of the biocontrol agents and some additives resulted in a significantly higher activity with respect to the single treatments applied separately, producing additive or synergistic effects. Some of the selected additives combined with a low yeast concentration (106 cells per ml) had comparable or higher efficacy than the biocontrol agents applied alone at a 100-fold higher concentration (108 cells per ml). Some organic and inorganic calcium salts, natural gums, and some antioxidants displayed the best results. In general, the effect of each additive was specific to the biocontrol isolate used in the experiments. Possible mechanisms involved in the activity of these beneficial additives and their potential application in effective formulations of postharvest biofungicides are discussed.

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